Gucy2e (BC057954) Mouse Untagged Clone

CAT#: MC220725

Gucy2e (untagged) - Mouse guanylate cyclase 2e (cDNA clone MGC:68216 IMAGE:5360206), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gucy2e
• Synonyms: GC-E; GC1; ROS-GC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC057954
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCTTGGCTCCTGCCAGCCGGAGGGCTTCCCGGCGCCGGGTTCTGTGTCCCTGCGCGGCAGTCTC
  CGTCCAGTTTCTCGCGGGTCCTGCGCTGGCCAAGGCCTGGGCTACCGGGACTCCTGCTACTGCTACTGCT
  CCCATCTCCTTCTGCCCTCTCTGCTGTGTTCAAAGTGGGGGTGCTGGGCCCCTGGGCTTGCGACCCCATC
  TTTGCACGGGCCCGACCAGACCTGGCTGCGCGTCTGGCCGCCAACCGCCTGAATCGTGACTTTGCTTTAG
  ACGGCGGCCCCCGGTTCGAGGTTGCGCTGCTCCCAGAGCCCTGCCTGACTCCGGGCTCACTAGGGGCTGT
  GTCCTCTGCGCTGTCTCGAGTCTCTGGCCTGGTGGGTCCGGTGAACCCCGCAGCCTGTCGGCCAGCCGAA
  CTGTTGGCTCAAGAAGCTGGAGTAGCGCTGGTGCCCTGGGGCTGCCCTGGCACGCGGGCGGCGGGTACTA
  CAGCCCCGGCGGTGACCCCCGCTGCAGATGCTCTCTACGTCCTCCTTAGAGCATTCCGCTGGGCGCGCGT
  GGCCCTGATCACTGCACCCCAAGACCTGTGGGTGGAGGCGGGACGCGCTCTGTCCACAGCACTCAGGGCC
  CGGGGTTTGCCAGTTGCCCTAGTGACTTCCATGGAGACTTCAGACCGGTCTGGAGCCCGGGAGGCCCTCG
  GGAGGATCCGAGATGGGCCTAGAGTTAGAGTAGTGATCATGGTGATGCACTCGGTGCTGCTGGGCGGCGA
  GGAGCAGCGCTACCTACTGGAAGCTGCAGAAGAACTGGCTCTGACTGATGGCTCCCTGGTTTTCCTGCCC
  TTCGACACGCTTCACTACGCTTTGTCTCCAGGCCCGGAGGCTCTGGCTGCATTTGTCAACAGCTCCCAGC
  TCCGCAGGGCTCACGATGCGGTGCTCACACTCACGCGCCGCTGTCCTCCTGGAGGCAGCGTGCAAGACAG
  CCTGCGCAGGGCTCAAGAACACCAGGAACTGCCCCTTGACCTCAACCTGAAGCAGGTCTCTCCGCTGTTT
  GGCACCATCTATGATGCTGTCTTCCTGTTGGCTGGGGGCGTGAAGAGAGCAAGAACAGCGGTGGGTGGTG
  GCTGGGTGTCAGGTGCATCTGTAGCCCGCCAAGTACGGGAAGCACAAGTCTCTGGCTTTTGTGGGGTCCT
  GGGAAGAACCGAGGAGCCCTCCTTTGTGCTGCTGGACACAGATGCATCCGGAGAACAGTTGTTCGCAACA
  CACCTGCTAGATCCTGTCTTAGGCTCCCTGCGTTCTGCAGGGACCCCCATGCACTTCCCTAGAGGTGGAC
  CTGCCCCGGGACCAGACCCTTCCTGCTGGTTCGATCCAGATGTGATCTGCAACGGAGGGGTGGAGCCAGG
  CCTGGTCTTTGTTGGCTTCCTCCTGGTGATAGGGATGGGACTGACTGGAGCCTTCTTGGCTCATTACTTG
  AGGCACAGGCTGCTACACATGCAGATGGCTTCCGGCCCCAACAAGATCATCTTGACGTTGGAAGATGTTA
  CTTTCCTCCACCCACCGGGAGGCAGCTCTCGAAAGGTGGTCCAGGGAAGTAGATCCAGTCTGGCTACCCG
  GAGCGCATCAGACATTCGCAGTGTCCCCAGCCAGCCCCAAGAGAGCACCAACGTTGGCCTCTATGAGGGG
  GACTGGGTTTGGCTGAAGAAGTTCCCAGGGGAACATCATATGGCTATCAGGCCAGCAACAAAGACAGCCT
  TCTCCAAGCTTCGAGAGCTCCGGCATGAGAATGTGGCTCTCTACTTGGGACTCTTCCTGGCGGGTACAGC
  AGACAGCCCTGCCACCCCTGGGGAGGGCATCTTGGCTGTGGTCTCAGAGCACTGTGCTCGGGGTTCCCTC
  CATGACCTCCTGGCCCAGAGAGAAATAAAGCTGGACTGGATGTTCAAGTCTTCCCTCCTGCTGGACCTCA
  TCAAGGGAATGAGATATCTGCACCATCGCGGTGTGGCCCACGGGAGGCTCAAGTCACGGAATTGCGTGGT
  GGACGGGAGGTTCGTGCTCAAGGTGACAGATCATGGCCATGGGCGACTGCTGGAAGCGCAAAGGGTGTTA
  CCGGAACCTCCCAGTGCAGAGGATCAGCTATGGACAGCCCCAGAGCTTCTTCGGGACCCCTCCCTGGAGC
  GCCGGGGAACTCTAGCTGGTGATGTCTTTAGTCTGGCCATCATCATGCAGGAGGTCGTGTGCCGCAGCAC
  CCCTTATGCCATGCTGGAACTAACGCCCGAGGGTAAGGTGGTTTGGGGCCAGATAGGCACCCTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC057954
• Insert Size: 1974 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057954
• RefSeq Size: 3213 bp
• RefSeq ORF: 2306 bp
• Locus ID: 14919
• Cytogenetics: 11 42.51 cM
• Gene Summary: Catalyzes the synthesis of cyclic GMP (cGMP) in rods and cones of photoreceptors. Plays an essential role in phototransduction, by mediating cGMP replenishment (PubMed:21598940). May also participate in the trafficking of membrane-asociated proteins to the photoreceptor outer segment membrane (PubMed:17255100).[UniProtKB/Swiss-Prot Function]