Fbxl17 (NM_015794) Mouse Untagged Clone

CAT#: MC220581

Fbxl17 (untagged) - Mouse F-box and leucine-rich repeat protein 17 (Fbxl17), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fbxl17
• Synonyms: 6330576B01Rik; AI452053; BB073797; C130023C01Rik; Fbx13; Fbxo13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220581 representing NM_015794
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCACCTTCTCTCGAAGGAGCCGCGTAACCGCCCGAGCCAGAAGAGGCCTCGCTGCTGCAGCTGGT
  GTCGCCGCCGGCGCCCTCTCCTCAGACTGCCCCGCCGGGCTCTGGCCAAGGCGTCCCCGCAGCCCGCGGC
  GCCCCGGAGCCGGGACTGCTTCTTCCGCGGGCCCTGCATGCTCTGCTTCATCGTGCACAGCCCCGGCGCG
  CCCGCCTCCGCCGGCCTAGAGGAGGAGCCGCCGCTCTCGCCACCGCCGCCGCCGCCGCGGGACGGGGCCT
  ACGCCGCCGTCTCCTCGCAGCACTTGGCGCGGCGCTACGCGGCCCTGGCCGCCGAGGACTGCGCCGCCGC
  CGCACGCCGCTTCCTGCTGTCCTCGGCCGCCGCCGCCGCCGCCGCTGCCTCGTCGCCCGCGTCCTGCTGC
  AAGGAGCTGGGGCTGGCCGCCGCCGCCGCCTGGGAGCAGCAGGGCCGGAGCCTCTTCCTGGCCGGCGTGG
  GGCCCGTGCGCTTCCTGGGACCGCTGGCCGCCGTGCAGCTCTTCCGGGCACCGCCGGCGCCGCCGCCGCA
  GGCCGAGCCCGCCACGGCCCTCGAGATGGTTTGCAAGCGGAAAGGGGCCGGGGTCCCCGCCTGCACCCCC
  TGCAAGCAGCCCCGTTGCGGCTGCGGGGGCTGCGGCGGGGGCGGCGGCGGCGGGGGAGGGCCCGCGGGAG
  GAGGTGCCTCGCCGCCGCGGCCGCCCGATGCCGGCTGCTGCCAGGCCCCGGAGCAGCCCCCGCCGCCGCT
  CTGCCCCGCGCCCGCCTCTCCCGCCTCAGAGTGTGCCCCCATCGTGGCCGCCGCCGGGGACACAGTCCGA
  GCCGGGGGCACCGCCCCCTCGTCTGCCCAGCAGCAGCCCGAGAGCGGCGACGCGGACTGTCAGGAGCCCC
  CCGAAAACCCCTGCGACTGTCACAGGGAGCCGCCCCCCGAGATCCCAGACATCAACCAACTGCCGCCGTC
  CATCCTGCTTAAGATATTTTCCAATTTGTCCCTGAACGAACGCTGCCTTTCTGCATCGTTGGTTTGCAAG
  TACTGGCGTGACCTTTGCTTAGATTTCCAGTTTTGGAAGCAGCTGGACCTTAGTAGTCGTCAGCAGGTTA
  CTGATGAATTATTGGAAAAAATTGCCTCCAGAAGTCAGAATATAATTGAGATTAACATTTCTGATTGTCG
  AAGTCTGTCTGACAGTGGCGTGTGTGTCCTAGCATTTAAGTGTCCTGGACTTCTTAGGTATACAGCCTAC
  AGGTGTAAGCAGCTTTCTGACACCTCAATCATTGCGGTCGCGTCTCACTGCCCTTTACTGCAGAAAGTCC
  ACGTAGGCAACCAGGACAAGCTCACCGATGAAGGACTTAAGCAGCTGGGCTCAAGATGCAGAGAACTCAA
  AGACATCCATTTTGGCCAGTGTTACAAGATCTCAGATGAAGGCATGATTGTCATAGCTAAGAGCTGCCTG
  AAGTTACAGAGGATATACATGCAGGAAAACAAATTAGTGACAGATCAGTCAGTGAAGGCATTTGCTGAGC
  ACTGTCCTGAACTCCAGTATGTTGGCTTCATGGGTTGTTCAGTTACTTCCAAAGGAGTCATTCACTTAAC
  CAAGCTAAGGAACCTGTCCAGCTTAGACCTCCGTCACATCACTGAACTGGATAATGAAACCGTGATGGAA
  ATCGTCAAGAGGTGCAAAAATCTCAGCTCTCTGAATCTCTGTCTGAACTGGATCATAAACGACAGGTGTG
  TGGAGGTCATTGCAAAGGAAGGACAGAACCTGAAAGAGCTGTACCTGGTGTCCTGTAAGATCACTGATTA
  TGCACTGATAGCCATTGGGCGATACAGCGTGACCATAGAGACTGTGGACGTCGGATGGTGTAAAGAAATC
  ACAGATCAAGGAGCCACCCTGATTGCACAGAGCAGCAAGTCCCTGAGATACTTGGGCCTGATGAGATGTG
  ACAAAGTCAATGAATTGACAGTGGAGCAGCTGGTGCAGCAGTACCCGCACATCACCTTCAGCACCGTTCT
  GCAGGACTGCAAGAGGACCTTGGAGAGAGCCTACCAGATGGGCTGGACCCCCAATATGTCCGCTGCCACC
  TCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_015794
• Insert Size: 2106 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015794.1, NP_056609.1
• RefSeq Size: 17001 bp
• RefSeq ORF: 2106 bp
• Locus ID: 50758
• UniProt ID: Q9QZN1
• Cytogenetics: 17 E1.1
• Gene Summary: Substrate-recognition component of the SCF(FBXL17) E3 ubiquitin ligase complex, a key component of a quality control pathway required to ensure functional dimerization of BTB domain-containing proteins (dimerization quality control, DQC). FBXL17 specifically recognizes and binds a conserved degron of non-consecutive residues present at the interface of BTB dimers of aberrant composition: aberrant BTB dimer are then ubiquitinated by the SCF(FBXL17) complex and degraded by the proteaseome (By similarity). The ability of the SCF(FBXL17) complex to eliminate compromised BTB dimers is required for the differentiation and survival of neural crest and neuronal cells (By similarity). The SCF(FBXL17) complex mediates ubiquitination and degradation of BACH1 (By similarity). The SCF(FBXL17) complex is also involved in the regulation of the hedgehog/smoothened (Hh) signaling pathway by mediating the ubiquitination and degradation of SUFU, allowing the release of GLI1 from SUFU for proper Hh signal transduction (PubMed:27234298). The SCF(FBXL17) complex mediates ubiquitination and degradation of PRMT1 (PubMed:28883095).[UniProtKB/Swiss-Prot Function]