Phactr4 (NM_175306) Mouse Untagged Clone

CAT#: MC220524

Phactr4 (untagged) - Mouse phosphatase and actin regulator 4 (Phactr4), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Phactr4
• Synonyms: 3110001B12Rik; AI527228; AW495572; C330013F19Rik; mKIAA4120; N28169
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220524 representing NM_175306
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACCCATCGGAAGAAGCAGAGCAGCCCTCTGGAGACCCAGGCATGGGCATGGACAGTGTGGAAG
  CAGGGGACACAACACCTCCCACCAAAAGGAAAAGCAAGTTCTCAGCGTTAGGCAAGATCTTCAAGCCGTG
  GAAATGGAGGAAAAAAAAAAGCAGTGATAAGTTCAAAGAGACATCAGAAGTTTTAGAGCGAAAAATATCT
  ATGCGAAAACCAAGAGAAGAACTGGTTAAACGAGGGGTTCTGTTGGAGGACCCCGAGCAGGATGGTGAGG
  ATTCAGGAAAGCTGAGCCATGCTGCATTAAAGAACGGACACACCACCCCCATAGGCAGTGCCAGGTCCTC
  TAGCCCAGTCCTGGTAGAAGAAGAGCCAGAGAGAAGTCTCAGGAACCTCACTCCAGAAGAGGAGTCAAAG
  AAGAGACTTGGCTCAACTGGAAGCCAGCCTAACTCTGAAGCCGAGCCTGGTCCTGAGCATGCACCTAAGC
  AGCCTCTGCTGCCCCCCAAGAGACCCTTGTCCTCTTCTTGTGAAGCAAAGGAGGTTCCGGCTGGCAGTAC
  AGCAAGGTCCGTCTCCTCCACCTCTGGCTCTACCACCGTGACATCTGCTGCCACCACTGCTGCCACAGAC
  ATGACAAAGACTGTTAAGTCCTTTGTGGGCCCCACCCCCGCTCCTGCCCCCGCCCCCAGGACTCTGCCTG
  CTGCTCCTGCCAGTGCTAACACTGCTGCCACCACTACAGCCCCTGCCAAGCAACCCCCCATCCCACCCCC
  TAAACCAGCTCAGAGAAACAGCAACCCCATCATTGCTGAACTATCCCAAGCAATGAACAGTGGCACAGTG
  CTGTCGAAACCATCCCCACCCTTGCCACCCAAGAGAGGCATTCCATCAACTTCGATACCCAGCTTGGAGC
  CTGCTGCTTCGTTCACAACTAAGACAGCAAATGATCAAAGAGAAAAAACTGTCTCTTTGTGTTTGGAACC
  ACCACTGATAATCCCGCCTTCGTCCCCATCACCCCCACTGCCTACCCACATACCTCCCGAGCCTCCACGG
  TCCCCTCTAGTGCCTGCTAAGACTTTTCAAATTGTGCCAGAGGTTGAGTTTTCCTCTTCTTCAGATCTAT
  TCCAGGACATTTCCCAGCAGGAGGATCAGAAAACAGAAGTCCCCAAGAAGATACAGGATCAGAGCTTTGG
  GGAGTCCCATATACCCTCCAGGCTGCCCCCACTCCCACTGCATATCCGAATTCAGCAGGCCCTTACCAGC
  CCCCTTCCTGTGACCCCTCCCTTGGAAGGCACTCACAGAGCCCATTCACTGCTTTTTGAGAACAGTGACA
  GCTTCTCCGAGGACACTGGTACCCTGGGTCGTACCAGGTCACTTCCCATCACGATTGAGATGTTGAAAGT
  TCCAGATGATGAAGAGGAAGAGCAGACCTGCCCGTTTGTTGAAGACGTGACGTCTACCTCAGCCACTCCT
  AGCCTACCACTGTGTCTGCGAGAGGAAGAGAAGGAGAGTGACTCAGATTCAGAGGGTCCCATTAAGTACC
  GAGATGAAGAAGAAGATGATGATGATGATGAAAGCCATCAGAGTGCCCTGGCCAACAGAGTGAAGAGGAA
  GGACACGCTGGCAATGAAGCTGAGCAGCAGACCCAGTGAACCAGAGACCAACCTGAATTCTTGGCCTCGT
  AAAAGCAAGGAGGAGTGGAATGAAATACGGCACCAGATCGGGAACACGCTGATCCGGCGGCTGAGTCAGA
  GACCAACAGCAGAAGAATTGGAGCAGCGGAACATACTACAGCCTAAAAATGAAGCTGATCGGCAAGCAGA
  GAAACGGGAAATCAAGCGTCGCCTCACTAGAAAGCTCAGTCAAAGGCCAACTGTGGCTGAACTGCTTGCC
  AGGAAGATTCTGCGGTTTAATGAGTATGTGGAAGTAACTGATGCTCATGACTATGACCGGCGAGCAGACA
  AACCATGGACCAAACTGACTCCTGCTGACAAGGCTGCCATAAGGAAAGAATTAAATGAATTTAAGAGCTC
  GGAGATGGAGGTTCATGTAGACAGCAAACACTTTACACGCTACCATCGCCCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175306
• Insert Size: 2085 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175306.4, NP_780515.2
• RefSeq Size: 4585 bp
• RefSeq ORF: 2085 bp
• Locus ID: 100169
• UniProt ID: Q501J7
• Cytogenetics: 4 D2.3
• Gene Summary: Regulator of protein phosphatase 1 (PP1) required for neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. Acts as an activator of PP1 by interacting with PPP1CA and preventing phosphorylation of PPP1CA at 'Thr-320'. During neural tube closure, localizes to the ventral neural tube and activates PP1, leading to down-regulate cell proliferation within cranial neural tissue and the neural retina. Also acts as a regulator of migration of enteric neural crest cells (ENCCs) by activating PP1, leading to dephosphorylation and subsequent activation of cofilin (COF1 or COF2) and repression of the integrin signaling through the RHO/ROCK pathway.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).