Rrn3 (NM_001039521) Mouse Untagged Clone

CAT#: MC220191

Rrn3 (untagged) - Mouse RRN3 RNA polymerase I transcription factor homolog (yeast) (Rrn3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rrn3
• Synonyms: AL023001; E130302O19Rik; R75565; TIF-1A; Tif1a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220191 representing NM_001039521
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCCGCTGCTTCACACGCGCTTGTCGGGCGATGTGACGGCAGCGGCCTCTGCCACGCTGAGTG
  CGTCGCGGACTGGGCTTTCCGATATGCTCGCGTTAGAGAGCGATTTCTTCAATTCTCCTCCGAAAAAAAC
  TGTTCGGTTTGGCGGAACTGTGACAGAAGTCTTACTGAAATACAAAAAGGGTGAAACAAATGACTTAGAG
  TTGTTGAAGAACCAGCTGTCTGATCCTGATATAAAGGATGACCAGATCATTAACTGGCTACTAGAATTTC
  GTTCCTCTGTCATGTACTTGACAAAAGACTTTGAGCAACTTATAAACATCATATTGAGATTGCCCTGGTT
  GAATAGAAGTCAGAGAGTGGTGGAGGAGTATTTGGCTTTTCTTGGTAATCTTGTGTCTGCACAGACTGTC
  TTCCTTAGACCATGTCTCAGCATGATTGCGTCTCATTTTGTACCTCCCCGAGTAATTGTCAAGGAAGGTG
  GCATAGATGTTTCAGATTCTGATGACGAAGATGACAATCTTCCTGCAATTTTTGACACATGTCACAGAGC
  CTTGCAAATAATAACAAGATATGTCCCATCGACACCATGGTTTCTAATGCCAATACTGGTGGAGAAATTC
  CCGTTTGTGAGGAAGTCCGAGAGAACATTGGAATGTTATGTTCATAACTTATTAAGGATAAGTTTATATT
  TCCCAACTTTGAGGCGTGAAATTCTGGAGCTTGTTATTGAAAAGCTACTCAAGTTAGATGTGAGTGTATC
  GCGGCAGGATATTGAAGATGCTGAAGAGAAAGCAGCTCAGACTTGTGGTGGGACAGATACCACAGAAGGA
  CTGTTTAATATGGATGAAGATGAGGACACTGACCCTGAGAAGAAAGCTGACCAGGAGCAGCCTAACCAGA
  TGGCTCATCCCACCGCAGAGCGCCTGGACGTCCTGCTGTGCTTGCTGCTGTCCTACATTGAGGATGTCTG
  CCGTGTGCACGGTAAAATTGATAACAATAAAACAAAGGATTTATACCGTGATCTGATATCCATCTTTGAC
  AAACTTCTGTTGCCCACACATGCCTCCTGCCATGTACAGTTCTTCATGTTTTTCCTCTGCAGCTTCAAGT
  TGGGATTTGCAGAAGCATTTTTGGAACATCTCTGGAAAAAGTTGCAGGATCCAAATAACCCCGCCATCAT
  CAGGCAGGCTGCTGCAAATTATATTGGGAGCTTTTTGGCCAGAGCTAAATTTATTCCTCTTATCACTGTG
  AAGACATGCCTGGATCTCTTGGTTAACTGGCTGCACATGTACCTTACTAACCAGGATTCGGGAACAAAGG
  CTTTTTGTGACGTTGCACTCCATGGACCATTTTATTCAGCCTGCCAAGCTGTGTTCTACACTGTTGTTTT
  TAGACACAAGCAGCTTTTGAGTGGAAACTTGAAGCAAGGTCTACAGTATCTTCAGAGTCTAAATTTTGAG
  CGCATTGTGTTGAGCCAGCTGAACCCACTGAAGATCTGCCTGCCGCAAGTGGTTAATTTCTTTGCTGCTA
  TCACAAATAAATACCAGCTGGTGTTCTGCTACACCATCATGGAGAGGAACAGTCGCCAGATGCTCCCCGT
  TATCCGCAGCACCGCTGGTGGAGACTCCGTGCAGACCTGCACCAACCCACTGGACACTTTCTTCCCCTTT
  GACCCTTGTGTGCTTAAGAGGTCAAAGAAGTTCATTGATCCTATTTATCAGATTTGGGAAGATGGGAGTG
  CTGAAGAGCTTCAGGAGTTTAAGAAATCTACTAAAAAGGAGGTAGTGGAGGATGAAGATGATGACTTTTT
  GAAAGGCGAGGTGCCCCAGAGTGACACAGTGACTGGCCTTACTCCGAGCTCCTTTGATACCCACTTCCAA
  AGTCCTTCCAGTAGTGTGGGCTCCCCTCCTGTGCTGTATATACCAGGCCAGTCTCCACTCCTCACAAGGA
  TCTATGATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001039521
• Insert Size: 1971 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039521.1, NP_001034610.1
• RefSeq Size: 3607 bp
• RefSeq ORF: 1971 bp
• Locus ID: 106298
• UniProt ID: B2RS91
• Cytogenetics: 16 A1
• Gene Summary: Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction.[UniProtKB/Swiss-Prot Function]