Fastkd3 (NM_027123) Mouse Untagged Clone

CAT#: MC220181

Fastkd3 (untagged) - Mouse FAST kinase domains 3 (Fastkd3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fastkd3
• Synonyms: 2310010B21Rik; AW048587
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220181 representing NM_027123
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTCATCACCCTGCGGAGGGCCTTTTGTCATAAGTCCATTCTTTGGATACCTGGAGCTGTGGTTG
  CTCTGAAAATTCACCCTGCCAGTCATGCCCCAAAGGCAGTCACAGATCGCCTGAGTGTGTGCTTCTGCTC
  CCTACAACCTGAGCTGTTCAGGGTCAGATTCCATCACGCATACTGTAAAAACTTCCACTCAGAAAAGGGA
  AATGACTTTCATCCCGTGGGCGAGCCATGGTCCTCCCAGGCGCAGGAATGGAACCAGCCAGGACAGAGTC
  TTCAAAACGAGGATGAAGAGATGCTTTTCAGGAGACTAAGCTACTTCACTTCCTTTGAGGAAGTGCTGAG
  TTTCATAAGTGCCCTGGACACCCTGCCTGTCCCTCTGGCAATGGCAGCTCTACTCAGGATCTGTGAGATA
  GGGAGGAGGGATGGTGAGCAAAGGCTGCCAGAAGGAGTGCTGGAGAACCGTGCCTTCCAGGCTCTTTGCC
  TCCGGTGTGAGCGAGACCCCTCACACCTGACCAATGCTGGTTTGGTGACAGCTTTGCAATCTCTGCTTAC
  GTTGTTGCCTGCAGATCCTCAAAGTAGCCTGATGCTGAGCCTGGTGGCAGAATGCCAACGTCGTCTCCAA
  AGAGGCAACCTCGAAGTCCACCATCTTTGTGTCCTTGGGGAAAGTCTGGCCATGCTTCAAGGTGCGAGTT
  GTGAGACCCTGAAGCTGGTTGTCCGTCAACTTCAAAGTAAAAGTGTGGAAACATTTGCTCCAGAGGAGAT
  CACGTCCGTTTATAGGATTCTGCAGGTGTGTCCTGAAGAAGTAGACAAACACCAGATGTTCTTAAACACA
  TTAAACAACTTCTCCATTTCAGTAGTTCCCTACCTGAGCCCTAAATCCATTAGCCATGTCCTCACCGCCT
  TAGTGGCCCTGGACCAGACTCACGCACTCCCACTGCTTATAAAGCTGGGCAAGTACGTTGTGAGATACAT
  CCCCCGTTTTACCAACGAAGAGCTCAGGAAGGTCTTGGAGGCGTTTGTCTATTTCGGACACAGTGACAGG
  TTCTTCACAGAGGCCTTAGAGCAACATGTGTCCGCCCTGTGCTTCTCCCTGGACCCCGCTGTGGCCAGCT
  CCGTGATGGGGTACTGCAGCAGGAAACGGATTCTCTCCAAGCCCATCTTCGACGTAGTCTCAGAGATCGT
  TGTTTGCCAGTGGGACAGACTTTCACCTAGTCAGATTGCCGAGCTAATTGAGCCATTTGGAAAGCTCAAT
  TACGTGCCACCAAACGCCCCTGCCCTGTTTAGGAAGGTAGAAAATGTGCTCTGTGCCCGTTTACATCACT
  TCCCACCAAAGATGCTACTGAGGCTCCTTCATTCATGTGCGCTGATTGAGCGACATCCCGTCAACTTCAT
  GTCAAAACTATTCAGCCCCTTTTTCCTTCAGCGGCTGCAAGGTAAAGAGTCATATTTGGACAGATTGAGC
  CTGGCACAGCTGACCCAACTTTTCCTAACCTCCGTCCTAGAGTGTCCGTTCTATAAGGGCCCCAAACTTC
  TTCCTAAATATCAAGTGAAGTCATTTCTCACTCCATGCTGCTCCTTAGAGACGCCCCTGGATCTCCATCT
  GTATAAGTCTGTGGTGATTGGACTGATTGATCTTTTAGGATCAAGACTGTATTTTGCTTCAAAGGTGTTG
  ACGCCCTATTACTACACTATAGATGTTGAAGTTAAATTAGATGAAGATGGATTTGTATTGCCGTGTACAG
  TGGATGAGGATATCCACAAAAGGGTGGCGCTGTGTATTGATGGGCCACAGCGATTTTGCTTGGACAGTAA
  ACACTTACTTGGAAAAGAGGCTACTAAGCAGAGACACCTGCGGTTACTTGGTTACCAGGTTGTTCAGCTT
  CCCTATCATGAGCTGGAGCTGCTTACATCAAGACTTGAGTTGGTGGACTATTTACAAAGGAAGCTGTTTT
  CTCAAAGCTCCGCTGTCCACTGGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027123
• Insert Size: 1986 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027123.4, NP_081399.3
• RefSeq Size: 2362 bp
• RefSeq ORF: 1986 bp
• Locus ID: 69577
• UniProt ID: Q8BSN9
• Cytogenetics: 13 B3
• Gene Summary: Required for normal mitochondrial respiration. Increases steady-state levels and half-lives of a subset of mature mitochondrial mRNAs MT-ND2, MT-ND3, MT-CYTB, MT-CO2, and MT-ATP8/6. Promotes MT-CO1 mRNA translation and increases mitochondrial complex IV assembly and activity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.