Fgd2 (NM_013710) Mouse Untagged Clone

CAT#: MC220172

Fgd2 (untagged) - Mouse FYVE, RhoGEF and PH domain containing 2 (Fgd2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fgd2
• Synonyms: Tcd-2; Tcd2; tcs-2; tcs2; ZFYVE4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220172 representing NM_013710
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGAGCCTGTGAGAAGCAGGACTCAGTGTGCAACCTGGTGGCTGTGTTTGAGAACAACAGGACTC
  CGGGAGAAGCACCTGGATCCCATAGCCTTGAGGACCAGCCCCATAGCCCTGAACACCAGCTGTCCCTGTC
  CCCAGAGCCTTGGGAGGCACCCCCTGTCAAGGAGGCCTTGAAGTCTGAATTCCGGCCAGTGAGCAGGACA
  TATCTGAGTTCCCTCAAGAACAAGTTATCGAGTGGGGCTTGGAGGAGATCCTGTCAGCCTGGGGTCAGCC
  CGGGGCCAGAGACACAGGAACCTGAGGAGAAGAGGGTCGTGCGAGAGCTTCTGGAGACGGAGCAGGCCTA
  TGTGGCTCGCCTGCACCTGCTTGACCAGGTGTTCTTCCAGGAGCTGCTGAGGGAGGCAGGCCGCAGCAAG
  GCCTTCCCTGAGGACGTGGTGAAGCTCATTTTCTCCAACATCTCCTCCATCTATCGTTTCCACGCCCAGT
  TCTTCCTTCCCGAGCTGCAGCGGCGCGTGGATGACTGGGCAGCCACGCCCCGCATTGGGGATGTGATCCA
  GAAGCTGGCCCCGTTTCTGAAAATGTACAGCGAGTACGTGAAGAACTTTGAGCGGGCCGCGGAACTGCTG
  GCCACGTGGATGGACAAGTCTCAGCCCTTCCAGGAGGTGGTCACCCGCATCCAGTGCAGCGAGGCCTCCA
  GCAGCCTGACCCTGCAGCACCACATGTTGGAGCCTGTGCAAAGAATCCCGCGGTACGAACTGCTGCTCAA
  GGAATATGTGCAGAAGCTGCCAGCCCAGGCCCCAGACCTCGAAGATGCCCAGAGAGCACTGGACATGATC
  TTCTCAGCTGCACAGCACTCCAATGCAGCCATTGCAGAGATGGAGCGGCTGCAGGGCCTGTGGGATGTGT
  ACCAGCGCCTGGGCTTGGAGGATGACATCGTGGACCCCTCCAACACCCTGCTCCGAGAGGGCCCTGTTCT
  CAAGATCTCTTTCCGCCGCAGCGACCCAATGGAACGCTACCTGGTTTTGTTCAACAACATGCTTCTGTAT
  TGTGTACCCCGAGTCCTCCAAGTGGGTGCCCAGTTCCAGGTGCGGACTCGCATCGATGTGGCCGGCATGA
  AGGTGCGGGAGCTGACCGATGCTGAGTTCCCACACTCCTTCCTGGTGTCCGGAAAGCAGCGCACACTGGA
  GCTGCAGGCCCGGTCCCGAGATGAAATGGTTTCCTGGATGCAGGCCTGCCAGGCAGCCATTGACCAGGTT
  GAGAAGCGGAGTGAGACCTTCAAGGCTGCTGTCCAGGGACCTCAGGGGGACACGCAGGAGCCCAAGCCAC
  AGGTAGAGGAGCTGGGTCTCCGAGCGCCTCAGTGGGTCCGGGACAAGATGGTGACCATGTGCATGCGCTG
  CCAGGAGCCCTTCAATGCCCTGACTCGTCGGCGCCACCACTGCCGGGCTTGCGGCTATGTAGTGTGTGCC
  AAGTGCTCTGACTACCGTGCGGAGCTGAAATATGACAGCAACAGGCCCAACCGAGTCTGCCTGACCTGCT
  ACACATTTCTCACTGGAAACGTACTCCCTCAAGGCAAGGAGGACAAGAGGCGGGGCATCTTGGAGAAAGA
  GGCCTCAGCAGCACCCGAGCAGAGTCTGGTGTGCAGCTTCCTGCAGCTCATAGGGGACAAGTGCAGCAGG
  AGCCTTCCCCGGAGCTGGTGTGTGATCCCCCGGGATGACCCCCTTGTCCTGTATGTCTATGCAGCCCCCC
  AGGACACAAAGGCTCACACCTCCATCCCCCTACTGGGCTATCAGGTGATTTCAGGGCCCCAGGGGGACCC
  TCGGGTTTTCCAGCTGCAACAGTCAGGCCAGCAGTACACCTTCAAGGCCGAGTCTGTGGAGCTGCAGGGC
  CGCTGGGTGACAGCTATCAAGCGTGCGGCCAGTGGCCGGACCCCTGAGGGACCTGACGAAGAAGATGTGT
  CTGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013710
• Insert Size: 1968 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013710.4, NP_038738.2
• RefSeq Size: 2631 bp
• RefSeq ORF: 1968 bp
• Locus ID: 26382
• UniProt ID: Q8BY35
• Cytogenetics: 17 A3.3
• Gene Summary: Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Activates JNK1 via CDC42 but not RAC1. Binds to phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 5-monophosphate, phosphatidylinositol 4-monophosphate and phosphatidylinositol 3-monophosphate.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of a coding exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1.