Foxn1 (NM_008238) Mouse Untagged Clone

CAT#: MC220062

Foxn1 (untagged) - Mouse forkhead box N1 (Foxn1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Foxn1
• Synonyms: D11Bhm185e; Fkh19; HFH-11; Hfh11; nu; nude; wh; Whn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220062 representing NM_008238
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTCGCTACTCCCTCCGCAGTCTGACGTCACACTTCCAGGCTCCACCCGACTGGAGGGCGAACCCC
  AAGGGGACCTCATGCAGGCTCCGGGCCTCCCAGACTCCCCTGCCCCACAGAACAAGCATGCTAACTTCAG
  CTGCTCGTCGTTTGTGCCTGACGGCCCTCCAGAGAGGACACCCTCACTGCCCCCACACAGCCCCAGCATC
  GCATCTCCAGACCCAGAGCAGATCCAGGGCCACTGCACAGCCGGACCCGGCCCGGGCTCCTTCCGCCTTT
  CTCCTTCAGAAAAGTATCCTGGCTTTGGCTTTGAGGAGGGCCCAGCAGGCAGCCCAGGGCGCTTCCTCAA
  GGGCAACCACATGCCTTTCCACCCCTACAAGAGGCACTTCCATGAGGACATCTTCTCTGAGGCCCAGACG
  GCCATGGCACTTGATGGACACTCCTTTAAGACTCAGGGGGCACTGGAAGCCTTTGAGGAGATCCCTGTGG
  ACATGGGCGATGCTGAGGCCTTCCTGCCTAGCTTCCCAGCAGAGGCTTGGTGCAATAAACTCCCTTACCC
  CAGCCAGGAACACAACCAAATTCTGCAGGGGTCAGAGGTCAAGGTCAAGCCCCAAGCTCTGGACAGTGGT
  CCTGGGATGTACTGCTACCAGCCTCCCTTGCAACATATGTACTGTTCTTCTCAGCCTGCCTTCCATCAGT
  ACTCCCCGGGTGGAGGCAGCTACCCTGTGCCCTACCTGGGCTCACCTCACTATCCCTATCAGAGGATTGC
  ACCCCAGGCCAACGCCGAAGGTCACCAGCCACTCTTCCCAAAGCCCATCTACTCTTACAGCATCCTCATC
  TTCATGGCCCTTAAGAACAGTAAGACCGGAAGCCTTCCAGTCAGTGAAATCTACAATTTCATGACGGAGC
  ACTTCCCTTACTTCAAGACTGCTCCTGATGGCTGGAAGAATTCTGTTCGCCATAACCTGTCCCTCAACAA
  GTGCTTTGAGAAGGTGGAGAATAAATCCGGAAGTTCCTCTCGAAAGGGCTGTCTGTGGGCCCTCAATCCT
  TCCAAAATCGACAAGATGCAGGAAGAACTGCAGAAGTGGAAGAGGAAAGACCCCATTGCTGTGCGCAAAA
  GCATGGCCAAACCAGAAGAGCTGGACAGCCTCATTGGAGACAAAAGGGAAAAACTGGGCTCTCCGCTGCT
  GGGCTGTCCACCCCCTGGGCTGGCAGGCCCAGGTCCCATCCGGCCCATGGCACCATCAGCTGGTCTTTCC
  CAGCCTCTGCACCCAATGCATCCAGCTCCAGGCCCCATGCCTGGCAAGAACCCCCTGCAGGACCTACTGG
  GTGGCCATGCTCCCTCCTGCTATGGGCAGACCTACCCACACCTTTCCCCCAGCCTGGCCCCTTCTGGACA
  CCAGCAGCCATTGTTCCCACAGCCAGATGGGCATCTTGAGCTGCAGGCCCAGCCAGGCACCCCCCAGGAC
  TCACCTCTACCTGCCCACACACCACCCAGCCACGGTGCCAAGCTGATGGCTGAGCCTTCCTCAGCCAGGA
  CCATGCACGATACTCTACTGCCAGATGGAGACCTTGGGACTGACCTGGATGCTATCAACCCTTCTCTCAC
  TGACTTCGACTTCCAGGGAAATCTGTGGGAGCAGCTGAAGGATGACAGCTTGGCCCTGGACCCCCTCGTA
  TTGGTGACCTCGTCGCCGACGTCATCCTCCATGTTGCCACCCCCACCAGCAGCCCATTGCTTCCCCCCAG
  GGCCTTGTCTGGCAGAAACAGGCAATGAGGCAGGTGAACTGGCACCTCCAGGCAGCGGCGGCTCCGGTGC
  TCTGGGAGACATGCACCTCAGCACTCTCTACTCCGCCTTTGTGGAACTGGAGTCCACGCCCTCCTCAGCA
  GCTGCCGGCCCTGCCGTGTACCTCAGTCCCGGCTCAAAGCCATTGGCTCTGGCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008238
• Insert Size: 1947 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008238.2, NP_032264.1
• RefSeq Size: 3273 bp
• RefSeq ORF: 1947 bp
• Locus ID: 15218
• UniProt ID: Q61575
• Cytogenetics: 11 46.74 cM
• Gene Summary: The protein encoded by this gene is part of the forkhead family or "winged-helix" transcription factors that are important in developmental processes, immune system regulation, metabolism, cancer and aging. This gene family has over 100 members, subdivided into classes (A-Q) based on phylogeny. The encoded protein is proposed to regulate development of the thymus and differentiation of keratinocytes. Mutations in this gene cause severe primary T-cell immunodeficiency and congenital alopecia. In mouse mutations of this gene underlie the phenotype of the nude mouse, which has been widely used as a model system in oncology, immunology, dermatology, and transplantation studies. In humans mutations in this gene have been correlated with T-cell immunodeficiency, the skin disorder congenital alopecia, and nail dystrophy. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Apr 2013]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.