Dus3l (NM_144858) Mouse Untagged Clone

CAT#: MC220035

Dus3l (untagged) - Mouse dihydrouridine synthase 3-like (S. cerevisiae) (Dus3l), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dus3l
• Synonyms: AI662135; AW557805
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220035 representing NM_144858
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGACGGCAGCGGAGAGCGGTGGTGGCGGTGACTCCGGGGTTGGCGCGTGCGAACGGGGGGTGG
  CGCCCATTAAAGCTCAGTACCGCACAACCAAAGAACGGTTCCACGAATATCTGGATGGAGACAAGCAGGA
  GGGTGCATGCCAGGAAGTGCCCACAGGAGACCCAGCTGAGCCAGGGGCCAAAAGAATTCGCCTGGAAGAT
  GGGCAGGAAAATGGGAAGACAGAGGTGGCAATTGAATCCCGTGAAAGGCAGGTGCCCAAGAGGGCCCGTG
  GCCAAAACAAAAGCCGGCCCCATGTGAAGCCAGCACACTACGACAAGGACAGGCTCTGCCCGTCCTTCCT
  CCAGGAGCCGGCCACCCCATGTGCATTTGGAGACCGTTGCCGCTTCCTGCACGATGTGGGGCGCTACCTG
  GAGACCAAACCGGCAGACCTGGGCCCCCGCTGCGTGCTCTTCGAAACCTTTGGCCGGTGCCCTTTCAGCA
  TGACCTGTCGCTTTGCTGGAGCACACCTGGGGCCTGAGGGGCAGAACCTGGTGCAGGAAGAGGTGGTGGC
  CCGCTGTGCACAGCTCCCTTCAGTGCGGAATGGCTTAGACAGGGCCCTGCAGCAGCAGCTGCGGAAGCGC
  CAAGTGTGCTTTGAGCGAGCTGAGCAGGCCCTGAACCGCCTCACCCAGAGCCCCATGCCCACTGTTGTCC
  CTGAGACCACTGTGGCCATGGCGACCCCAAAGCAGAATAGCTGCCATGCCCAGCTAGATACCGTAGGAGG
  GGCTGGCACCCCGCAGAGCAGCCCTGTGCCAACCTGTGGCCCCTTGACAGACGAGGACGTGATCCGGCTG
  CGGCCCTGTGAGAAGAAGCGGCTAGACATCAGCGGAAAGCTGTATCTGGCTCCTTTAACCACGTGTGGAA
  ACCTACCCTTTCGCCGCATTTGCAAGCGCTTTGGCGCGGATGTGACCTGCGGTGAGATGGCCGTGTGTAC
  GAACCTGCTCCAGGGACAGATGTCTGAGTGGGCTTTGCTCAAGCGCCACCCCTGTGAGGACATCTTTGGG
  GTGCAGCTGGAGGGCGCGTTCCCTGACACCATGACCAAATGTGCCGAGCTCCTCAACCGCACCATTGACG
  TGGACTTCGTGGACATCAACGTGGGCTGCCCCATCGACCTCGTCTACAAGAAGGGTGGTGGCTGTGCACT
  CATGAATCGCTCCGCCAAGTTCCAACAGATTGTGCGGGGCGTGAATGAGGTGTTGGACGTCCCACTGACT
  GTGAAGATGCGCACAGGTGTCCAGGAGCGTGTGAGCCTGGCACACCGACTGCTGCCCGAACTGCGGGACT
  GGGGTGTCGCACTGGTCACGCTCCATGGCCGCTCCCGGGAACAGCGCTACACCAGGCTGGCTGACTGGCC
  CTACATAGAACAGTGTGCCAAGGTGGCCAGCCCCATGCCCCTGTTTGGAAATGGGGACATCCTTTCGTTT
  GAGGACGCCAACTGTGCCATGCAGACAGGCGTTGCGGGGATCATGGTTGCCCGTGGTGCCTTGCTCAAGC
  CCTGGCTGTTCACGGAGATCAAAGAACAGAGGCACTGGGACATCTCGTCCTCCGAGCGCCTGGACATCCT
  GAGGGACTTCACTCACTACGGTTTAGAACACTGGGGCTCCGACACACAGGGCGTGGAGCGGACCCGGCGC
  TTCCTGCTTGAGTGGCTCTCTTTTCTTTGCAGGTATGTGCCCGTGGGCCTGCTCGAGCGACCCCCACAGA
  GGATCAATGAGAGGCCACCCTATTACTTAGGCCGTGACTACCTGGAGACACTGATGGCCAGCCAGCAGGC
  TGCAGACTGGATTCGCATCAGTGAGATGCTGCTTGGACCAGTACCTCCTGGCTTCGTCTTCCTGCCCAAG
  CACAAGGCCAATGCCTACAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_144858
• Insert Size: 1914 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144858.2, NP_659107.2
• RefSeq Size: 2321 bp
• RefSeq ORF: 1914 bp
• Locus ID: 224907
• UniProt ID: Q91XI1
• Cytogenetics: 17 D
• Gene Summary: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).