Ripor2 (NM_178658) Mouse Untagged Clone

CAT#: MC219937

Fam65b (untagged) - Mouse family with sequence similarity 65, member B (Fam65b), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ripor2
• Synonyms: 1700108N18Rik; 6330500D04Rik; AI225904; E430013J17Rik; Fam65b; RP23-461O24.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219937 representing NM_178658
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAATTCCTTGACCCTGAGGATCTTCTGGATGAAGAGGATGACATTTTTGGCGAAGGACTGCCAACCA
  GACTCCCAGAAATCATGCTGGTGGGATCCCAGTCCTTCTCTCCTGGCGGGCCCAATGGCATCATCCGGAG
  CCAGTCCTTCGCAGGTTTCAGTGGGCTGCAGGAAAGGCGGTCCAGGTGTAACTCCTTCATTGAAAATGCC
  TCGGCTCTCAAGAAGCCTCAGGCCAAGCTGAAGAAAATGCATAATCTTGGACACAAAAATAACAACACTC
  CCAAGGAGCCTCAGCCGAAGAGAGTGGAGGAGGTCTACCGGGCCTTGAAGAACGGGCTTGATGAGTACCT
  GGAGTTTCACCAGACGGAGCTGGACAAATTGACTGCTCAATTAAAAGATATGAAGAGAAACTCTCGCCTG
  GGTGTGCTGTATGACCTAGACAAGCAAATTAAAACGATTGAGAGATACATGCGACGCCTGGAGTTTCACA
  TTAGTAAGGTAGACGAGCTCTATGAAGCCTATTGTATCCAACGGCGTCTCCAAGATGGTGCCAGCAAAAT
  GAAGCAAGCCTTCGCGACGTCCCCTGCCAGCAAGGCTGCCCGGGAGAGTCTGTCTGAGATCAACCGCAGC
  TACAAGGAATACACGGAGAACATGTGTGCCATTGAAGCGGAGTTGGAGAGTCTGCTGGGAGAATTCTCCA
  TCAAGATGAAAGGTCTGGCTGGCTTTGCCCGCCTCTGTCCTGGAGATCAATATGAAATTTTCATGAAGTA
  TGGTCGGCAGAGGTGGAAACTGAAGGGCAAAATAGAAGTGAATGGTAAACAGAGCTGGGATGGAGCAGAA
  ACCGTCTTCCTGCCCCTCATAGTTGGGTTCATTTCAATCAAGGTCACAGAACTCAAAGGACTGGCGACTC
  ACATCCTGGTAGGCAGTGTGACCTGCGAGACCAAAGAGCTGTTTGCAGCCCGACCCCAGGTGGTGGCTGT
  TGACATCAATGACCTTGGTACCATCAAACTGAACCTGGAAATCACCTGGTATCCCTTTGACGTGGAGGAC
  ACAACCCCATCGTCGGGCCCTGGGAACAAGACAGCTGCTCTCCAGCGAAGGATGTCCATGTACAGCCAGG
  GCACCCCAGAAACACCTACCTTCAAAGACCAGTCCTTCTTTTCAAATCTGCCAGATGACATCTTTGAGAG
  TGGAAAGGCTGCAGAGGAGAAAAGGCCACTGTCCCTCAGCTTCAGTGACCTGCAGGATGGAGACTGTGTC
  TTCACCTCCAGCTCAGCCACCTCCCCCTCCAGTTCACACTCAGCCCATCCAGAGATTACCATCACCCCCG
  CAGAGCTTACCCACAGCAGCCTGTCCTCACAAAATGAGGGCACCGAGGACAGCAGCTCAGCGTCTTCCAG
  GAATTCCTTGGGTGAGGACCATGAGCCCAAGTCCCACCCAAAGAGTGATACGGTAGAGCCCGGGAAGCCG
  GGTGTGGCTACAAGGTCTGGTACCGAGAGCCTGTTTCTGGAGAGTAGTGTGGCCGAGGCTCTCCTGCAGG
  AATCGGATGAGGCCTCTGAGCTCAAGCCCGTGGAACTGGACACTTTTGAAGGAAACATCACAAAGCAACT
  GGTGAAGAGGCTCACCTCGGCTGAGGGGCCCGTCACTACTGACAAGCTTTTCTTTGAAGGCTCTGTTGGT
  AGCGAGTCTGAGGCTGGCCGGTCCTTTCTGGATGGCAGCCTGGAAGATGCCTTCAATGGACTCTTCCTTG
  CATTAGACCCACACAAGAAGCAGTACAAAGAGTTCCAGGATCTGAACCAAGAAGTCACTCACTTGGATGA
  TGTTCTCAAAGATGCTAAACATCTTGAGGATCAGAGACTCAATGATGCTGCTTCCCGGATGGAGATCACA
  GAGGGTGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178658
• Insert Size: 1902 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178658.5, NP_848773.3
• RefSeq Size: 3161 bp
• RefSeq ORF: 1902 bp
• Locus ID: 193385
• UniProt ID: Q80U16
• Cytogenetics: 13 A3.1
• Gene Summary: Acts as an inhibitor of the small GTPase RHOA and plays several roles in the regulation of myoblast and hair cell differentiation, lymphocyte T proliferation and neutrophil polarization (PubMed:25588844, PubMed:27269051). Plays a role in fetal mononuclear myoblast differentiation by promoting filopodia and myotube formation (PubMed:17150207). Maintains naive T lymphocytes in a quiescent state and prevents chemokine-induced T lymphocyte responses, such as cell adhesion, polarization and migration (By similarity). Involved also in the regulation of neutrophil polarization, chemotaxis and adhesion (PubMed:25588844). Required for normal development of inner and outer hair cell stereocilia within the cochlea of the inner ear (PubMed:27269051). Plays a role for maintaining the structural organization of the basal domain of stereocilia (PubMed:27269051). Involved in mechanosensory hair cell function (PubMed:27269051). Required for normal hearing (PubMed:27269051).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks several exons, and uses alternate 5'- and 3'-terminal exons, compared to variant 1. The encoded isoform (3) is shorter and has distinct N- and C-termini, compared to isoform 1.