Zic5 (NM_022987) Mouse Untagged Clone

CAT#: MC219831

Zic5 (untagged) - Mouse zinc finger protein of the cerebellum 5 (Zic5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zic5
• Synonyms: 1700049L20Rik; Opr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219831 representing NM_022987
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAGCCCCCTTTGAGCAAGAGGAACCCGCCAGCGCTGAGATTAGCGGATTTGGCAACGGCTCAGG
  CCCAACAGCTTCAGAATATGACAGGCTTCCCGGTGCTGGTCGGCCCGCCCGCCCACTCCCAACGCCGCGC
  GGTCGCCATGCACCTCCACCCGCGGGACCTAGGCACTGACCCCGGCGTAGCCAGCACTGCGCTCGGACCC
  GAGCACATGGCACAGGCCAGCGGGCAGGGCCCCTGTCCTCCTTCCCAGGGGCTCCCCGGGCTGTCCCAGG
  TTCCCGCACCAGCCGCCCGCTCCGTGGCCTCAGGGACTCACCCAGGCGCCCGCACCCACCCCGACGGCGG
  GGGCAGCAGTGGCGCGCAGGCCTCGGCGCCCCCGCCTCCAGCCCCTCCTCTTCCTCCCTCCCAGTCCTCC
  TCTCCCCCTCCCCCGCCTCCTCCTCCTCCTGCCCTCTCGGGCTACACTGCCACCAACAGTGGCGGCGGCA
  GCAGCAGCGGCAAAGGCCACAGCAGGGACTTCGTCCTCCGGAGGGACCTTTCCGCCACGGCCCCCGCGGC
  GGCCATGCACGGGGCCCCGCTCGGAGGGGAGCAGCGGTCCGGCAGCAGCTCCCCCCAGCACCCGACCCCA
  CCTCCCCACCCGGCCGGGATGTTCATCTCGGCCAGCGGCACCTACGCGGGCCGGGACGGCGGCGGCTCCG
  CGCTCTTTCCCGCGCTGCACGACTCTCCCGGGGCTCCCGGTGGCCACCCGCTAAACGGCCAGATGCGCCT
  TGGGCTGGCGGCCGCCGCGGCGGCTGCGGCCGAGCTTTACGGCCGCGCCGAACCACCCTTCGCTCCGCGC
  TCAGGGGACGCGCACTATGGGGCGGTGGCGGCCGCCGCGGCCGCCGCCCTGCACGGCTACGGAGCCGTGA
  ACTTAAACCTGAACCTGGCTGCGGCAGCGGCGGCCGCAGCGGCCGCGGGGCCCGGGCCCCACCTGCAACA
  CCACGCGCCGCCCCCGGCGCCACCGCCGGCGCCCGCGCCGCACCCGCACCACCCCCACCTCCCGGGGGCG
  GCCGGGGCCTTCCTGCGCTACATGCGGCAGCCAATCAAGCGGGAGCTCATCTGCAAGTGGCTGGACCCAG
  AGGAGCTGGCCGGTCCGCCGGCGTCCGCGGACAGCGGCGTCAAGCCCTGCTCCAAAACTTTCGGCACCAT
  GCACGAGCTGGTGAACCACGTCACAGTGGAGCACGTGGGCGGCCCGGAACAGAGCAGCCACGTCTGCTTT
  TGGGAGGACTGTCCGCGCGAGGGCAAGCCCTTCAAGGCCAAGTACAAGCTCATCAACCACATCCGCGTGC
  ACACCGGCGAGAAGCCCTTCCCCTGCCCGTTCCCGGGCTGCGGCAAGGTCTTCGCGCGCTCCGAGAACCT
  CAAGATCCACAAGCGCACTCATACAGGGGAAAAGCCTTTCAAATGTGAATTTGACGGTTGTGATAGGAAG
  TTTGCCAATAGCAGCGATCGAAAGAAACACTCCCATGTCCACACCAGCGACAAGCCCTACTACTGTAAGA
  TTCGAGGCTGTGATAAATCGTACACCCACCCAAGCTCTCTGAGGAAGCACATGAAGATTCACTGCAAGTC
  CCCCCCACCTTCGCCTGGAGCTCTTGGCTACTCGTCGGTGGGGACTCCCGTGGGTGACCCTTTGTCCCCT
  GTGCTGGACCCAACCAGGAGTCGATCCAGCACTCTGTCCCCTCAGGTGACCAACCTCAATGAGTGGTATG
  TTTGCCAGGCCAGTGGGGCCCCCAGCCATCTCCACACACCTTCCAGCAATGGAACCACCTCTGAGTCTGA
  AGATGAGGAAATGTATGGGAACCCTGAAGTCATGCGGACGATACATTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_022987
• Insert Size: 1869 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022987.3, NP_075363.1
• RefSeq Size: 2383 bp
• RefSeq ORF: 1869 bp
• Locus ID: 65100
• UniProt ID: Q7TQ40
• Cytogenetics: 14 E5
• Gene Summary: Essential for neural crest development, converting cells from an epidermal fate to a neural crest cell fate. Binds to DNA.[UniProtKB/Swiss-Prot Function]