Themis3 (NM_028998) Mouse Untagged Clone

CAT#: MC219102

Themis3 (untagged) - Mouse RIKEN cDNA 9130404H23 gene (9130404H23Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Themis3
• Synonyms: 9130404H23Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219102 representing NM_028998
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAAACTTGGGACTCTTACATCAGTTCCTTAAATCAGAACTCTTTGCCCAGACAAGTGGAGGTGA
  CCCAGGGAAGATATTCAGGGTCACTTGAAAACCTGTCATTCTCCAAAGGGGATATCATCACGGTGGTGGA
  CCTGGAGCCAGTTTTCGTCAGAGCAGAACTGAAGGACGGTGATCAAGTTCTGGACGTGGTCACTATACCT
  CTTAGATATGAAGGAAACTTCCAACTGATGGCAGACCCGGTTTCTTTTGAAACTGTGGCTGATTTAACCC
  GTTCTGTCCGACTTCCCCAGAGTCCTGTGGCCCCTAGAAGTCCTCCCCGCTTCCAGAACTCAATCCCCAT
  CTCCGCAGACAATTTGCCCATGAAGCTCAGGAAAGGAGAAACACTGTCCTTAATTGGCTTTCAAGAGAGT
  CGGGGGAGGAGGCTGCTGCAATGCAAGGTGCTGAGGAAGAAGCCACCGCTGACAGTGCTCCTCCCCATGG
  ACTGCAGGGGCCACTTCCTGGAGTGCCAGGACGACAGGTTCTACTCCATTGACACCATCGTGAGGTGGAA
  GATGCTTGCAGGGAGGAAACGCACGGTTAGAGTGCAAGCAAGACATCACCCGAAGCTCCTTGGCCCCCTG
  GTTCCAGAGCACTTTCGTGGACATTTAGTTTTGTATCCCTGTTTCTCAGTGACGGCAAATCTGCTAGGTG
  AGACCCGGGTTAGCATCCCCTCCGATCTGGATATCAGTGTTACTGAGATTGCCCGCCTCGATCGTAAGCC
  CAGGACAACGATGAGACAAATATACAGCATGGAAGAAAGCAAGTTCCCAGTGAGAGTTAAAATAATGAAT
  GTGGTCCAGTCGGAGAGCAAGGAGTACCCAAAGCCTCTGAAACGTGGGCAGCTCTTAACCATCCTCAAGA
  CTGAAGAGGTGAAAAAATTCGTTGCCTCTGAGATTTCCCAAGGAAAGAAAGGGAAGTGTTTTCTCGTCCC
  ATACACCTATCAGGGCCTGGTGCTTAGGAGAGGTCGTTATTTTTATGCTGTCTCTGATGTTGCTGCTGCC
  ATGAAGCATGGAGAGCTCTGCTTCCAGGCCTCCCAGGACTACACCTCCTACCTGGGATCGTTTGCATCTT
  TCAGAGCCAACGAGTGCTTCCTGGCCCTGAAGAAGAGTGTGGTTTCCGCCGAGATTCACGGTGAGCTGCA
  CAGGGTGGAAGTGCTCAAGTGCCTGAACATTGCCACTAAAGCCCACGTCAAACTGCCCTTGTTTGCCGTA
  GGTAAGTTCCTGGAGCTGTTTGACGGCGCTAGGCCAGGGACCCTGCAGGAGCTATGCCAAGTCACAAGAC
  TGCCGTGCCACGTCAGAGTGACCAGCCCAGATCCCTCCATGACTGTAGACCCTCTATATGGAACTAAGGA
  ACTGAGAATTGAAAATGTCATCATTGAACAATGCCTCATAGCCAAAGATGAACCAACACTGGAGGACATA
  ATCTCCTCAGCAGACATGTACAGGGAGTGGCCAGAAACCACATTTGAAATCCCGATTGAGAAGATAAGCT
  GTGAGGTGTTAGTGGTAGAAGAGCGTTCGTGGATAGCTGATGTCAGGAAAGAGAGATGCAGGCCTCTCCA
  GAGCATCCAGGAGGTCACTAAGGAGTCTCTGGCCTTTAGTAATTGCTTGGTTATTCGCCGTCCACCACCA
  CCTGTTCCAAAGCCCAGAAGTTTGTTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028998
• Insert Size: 1710 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028998.1, NP_083274.1
• RefSeq Size: 1798 bp
• RefSeq ORF: 1710 bp
• Locus ID: 74556
• UniProt ID: Q9CU24
• Cytogenetics: 17 E1.1