Slc9b1 (NM_028946) Mouse Untagged Clone

CAT#: MC219100

Slc9b1 (untagged) - Mouse Na+/H+ exchanger domain containing 1 (Nhedc1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc9b1
• Synonyms: 1700094G20Rik; 4933424B12Rik; 4933425K02Rik; mtsNHE; Nhedc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219100 representing NM_028946
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAGCACGACGTAGAATCAAACAAGAAGGATGATGGTTTCCAATCATCTGTAACTGTTGAGATGT
  CCAAAGATCCTGATAGTTTTCATGAAGAAACTGTGGAACCAAAACCAGAACTGAAGGAACCGGAACCAAA
  GGAACCAGAACCGAAGGAACCAGAAAGGAAGGAACCAGAAAGGAAGGAACCAGAAAGGAAGGAACCAGAA
  AGGAAGGAACCAGAAAGGAAGGTACCAGGAAGAAGGGAAACACAAACGAAGGAAACACAAACAACCGAAA
  TAGAAAGGAAGGAAACAAAAAAGAAGAGAGGGACAAATAGTTACTGTCCTCCACAAGGAACAATAAATAA
  AACAATTACAGATGGAGCTGCACTCATTGCACTATGGACTCTACTCTGGGCCCTTATAGGTCAAGAAGTT
  CTCCCTGGTGGAAATTTGTTTGGACTTGTAGTTATTTTTTACAGTGCTTTCCTTGGGGGTAAAATTTTAG
  AATTCATTAGAATACCTGTAGTGCCTCCCCTCCCACCTCTCATTGGGATGTTACTGGCTGGTTTCACCAT
  CAGGAATGTTCCGATTATTTATGAATTTGTCCATATTCCTACCACATGGTCTTCAGCTTTAAGAAACACT
  GCCCTTACTATTATCCTAGTACGAGCTGGGCTTGGACTGGATCCACAGGCCTTGAAGCATCTGAAGGGAG
  TCTGTCTGCGCTTGTCATTTGGTCCATGCTTCCTAGAAGCCTGCTCAGCCGCTCTCTTCTCCCACTTCAT
  TATGAACTTTCCTTGGCAATGGGGGTTCCTACTAGGTTTTGTCCTAGGCGCTGTCTCTCCTGCTGTTGTC
  GTCCCCAACATGCTAATGTTGCAGGAGAATGGGTATGGTGTTGAGAAAGGCATCCCGACCTTGCTGGTGG
  CAGCTAGCAGCATGGACGACATTGTGGCCATCACTGGATTCAACACATTCTTGAGCATAGTCTTTTCCTC
  GGGTAGTGTGATTAGCAACATCCTATCGTCCTTGCGGGACGTTCTTATTGGGGTGCTGGTAGGAATTGTT
  ATGGGAGTTTTTGTTCAGTATTTTCCAAGTGGAGATCAGGAGAGACTTACGCAGAGGCGAGCCTTCCTAG
  TTCTGAGTATGTGTATTTCTGCTGTCTTAGGCTGCCAGCACATTGGCTTACATGGATCTGGAGGACTGGT
  CACACTAGTATTGTCGTTCATGGCAGCAAAAAGATGGGCTGAAGAGAAGGTTGGAATCCAAAAAATTGTT
  GCAAACACATGGAATGTTTTTCAGCCGCTTCTTTTTGGCTTAGTTGGAACAGAAGTATCTGTTGAATCTC
  TTGAATCAAAAACCATTGGCATGTGCCTTGCTACCTTGGGTTTGGCACTATCTGTTCGAATTTTATCCAC
  TTTTGTACTGATGTCTTTTGCTAACTTCCGTTTTAAGGAGAAAGTATTTATTGCTTTATCATGGATCCCT
  AAAGCTACAGTCCAGGCTGTCTTAGGACCTCTGGCTCTAGAAACAGCAAGGGTCATGGCACCCCACTTGG
  AAGGATACGCGAAGGCTGTGATGACAGTGGCCTTCCTAGCCATCCTGATTACAGCTCCAAATGGAGCCCT
  CCTCATCGGCATACTGGGACCCAAAATCCTTGAACAGAGTGAGGTGACATTCCCTTTGAAAGTTGAGCTC
  TCGAACTTCCATCATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028946
• Insert Size: 1698 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028946.3, NP_083222.2
• RefSeq Size: 2245 bp
• RefSeq ORF: 1698 bp
• Locus ID: 74446
• Cytogenetics: 3 G3
• Gene Summary: Sperm-specific Na(+)/H(+) exchanger involved in intracellular pH regulation of spermatozoa (PubMed:20036903). Involved in sperm motility and fertility (PubMed:20036903, PubMed:27010853, PubMed:19409551).[UniProtKB/Swiss-Prot Function]