Ogfod1 (NM_177767) Mouse Untagged Clone

CAT#: MC218900

Ogfod1 (untagged) - Mouse 2-oxoglutarate and iron-dependent oxygenase domain containing 1 (Ogfod1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ogfod1
• Synonyms: 4930415J21Rik; AA387199; AA939912; AW061076; mKIAA1612
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218900 representing NM_177767
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGGGAAGCGGCCGGCGGATCCTGGCCCAGCGCGGCCAATGAAGAAGGGAAAGAAGCAGGTATCGG
  CAGAGTTTTCGGACGCTGTCACTGAAGAAATCCTGAGGAAGCAGGTGGCTGAGGCCTGGAGCTGCCGGAC
  ACCGTTCAGCCATGAAGCTATTGCCTTGGACATGGATCCTTTCCTTCACTGTGTGATCCCAAACTTCATC
  CAAAGCCAAGACTTCTTGGAAGGACTTCACAAAGAGCTTTTGAGCCTGGACTTCCATGAAAAGTACAATG
  ATTTATATAAGTTCCAACAGTCTGATGATTTGAAGAACCGAAAAGAGCCCCATATCTCAGCTTTAAGGAA
  ACTTATGTTTGAAGATTTTCGGGCCTGGCTTTCTAAGGTTTCCGGCATTGACCTAGAACCAACCATTGAC
  ATGTCGTGTGCGAAATACGAGTTCACTGATGCTCTGCTCTGCCATGATGATGAACTGGAGGGGCGCCGGA
  TCGCTTTCATTCTGTACCTGGTTCCTTCTTGGGACAGGGACTTGGGGGGCACCCTGGACCTTTATGACAC
  TGATGAACACTTGCAGCCAAAGCAGATTGTCAAGTCTCTTATCCCTTCCTGGAACAAACTGGTTTTCTTT
  GAAGTGTCTCCAGTATCCTTTCACCAGGTGTCTGAAGTCCTGTCTGAAGAAACATCACGTTTGTCTATAA
  GTGGCTGGTTTTATGGTCCTTCATTAACCAGGCCTCCTACCTACTTTGAGCCCCCAATCCCTCGAAACCC
  TCACATCCCACAAGATCATGAAATTTTATATGAGTGGATCAACCCTGCTTACCTGGAAATGGATTATCAA
  ATGCAAATTCAAGAAGAATTTGAAGAAAGGTCTGAAATTCTCCTGAAGGAGTTTCTTAAGCCTGAGAAAT
  TTGCAGAGGTCTGTGAAGCCTTGGAGAAAGGAGATGTGGAATGGAAAAGCCATGGTCCCCCAAACAAAAG
  GTTTTATGAGAAAGCAGAAGAAAATAACCTCCCTGACGTGCTGAAGGAATGCATGGGCCTGTTCCGCTCT
  GAGGCGCTCTTCCTGCTGCTCTCCAACCTCACTGGCCTGAAGCTTCACTTCTTGGCCCCTTCAGAAGATG
  ATGAGACTGAGGAGAAAGGAGAGGGGGAGACAGCCAGTGCTGCTGCTGGCACTGAAGAAGGGACTAGCCG
  ACGCCCCTCGGGGCCAGAGAATAATCAGGTGGCCGCTGGCAGTCACAGTCAAGAGAACGGTGAACAGGCA
  GACCCAGAAGCACAGGAAGAGGAAGCAAAGAAAGAATCGAGCGTTCCCATGTGCCAGGGGGAGCTGAGGC
  GTTGGAAGACTGGCCATTACACATTAGTCCACGACAACACCAAGACTGAATTTGCCCTGGACCTGTTTCT
  GTACTGTGGCTGTGAAGGCTGGGAGCCCGAATATGGCGGCTTTACCTCCTACATTGCTAAAGGTGAAGAT
  GAAGAGCTGCTCATAGTGAATCCAGAAAACAACTCTTTGGCGTTGGTCTACAGAGACAGAGAAACGCTGA
  GGTTTGTGAAACACATCAACCACCGCAGCCTAGAGCAGAGCAAAGCCTTCCCAAGCAGAAGCGGCTTCTG
  GGACTTCGCATTCATCTACTATGAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177767
• Insert Size: 1638 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177767.4, NP_808435.3
• RefSeq Size: 5329 bp
• RefSeq ORF: 1638 bp
• Locus ID: 270086
• UniProt ID: Q3U0K8
• Cytogenetics: 8 C5
• Gene Summary: Prolyl 3-hydroxylase that catalyzes 3-hydroxylation of 'Pro-62' of small ribosomal subunit uS12 (RPS23), thereby regulating protein translation termination efficiency. Involved in stress granule formation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.