Stam (NM_011484) Mouse Untagged Clone

CAT#: MC218776

Stam (untagged) - Mouse signal transducing adaptor molecule (SH3 domain and ITAM motif) 1 (Stam), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Stam
• Synonyms: STAM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218776 representing NM_011484
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCTCTTTGCCACCAACCCCTTCGACCAGGATGTTGAGAAAGCAACTAGTGAGTTGAATACTGCTG
  AGGACTGGGGCCTCATCTTGGATATTTGTGATAAGGTTGGCCAATCTCGCACAGGACCTAAAGATTGTCT
  TCGTTCCATTATGAGAAGAGTGAACCATAAAGACCCCCATGTTGCTATGCAAGCCTTAACTCTTCTGGGA
  GCATGTGTATCAAACTGTGGCAAAATTTTTCACTTAGAAGTATGTTCAAGAGACTTTGCTAGTGAAGTAA
  GCAATGTATTAAATAAGGGTCATCCTAAAGTATGTGAAAAATTAAAGGCTCTTATGGTTGAATGGACAGA
  TGAATTTAAGAATGATCCACAGCTTAGTCTGATATCGGCAATGATCAAGAACCTTAAGGAACAAGGTGTA
  ACATTCCCAGCTATTGGCTCTCAGGCTGCAGAACAAGCAAAAGCAAGCCCAGCTCTCGTAGCCAAGGACC
  CTGGGACTGTGGCTACCAAAAAGGAAGAAGAAGATTTAGCCAAAGCTATTGAGTTGTCCCTGAAGGAGCA
  AAGGCAGCAGTCAGCCCCCGTGTCCACGCTGTACCCCAGCACATCTAACCTCCTCACTAACCACCAGCAC
  GAAGGCAGGAAAGTCCGCGCCGTGTACGATTTTGAAGCCGCTGAGGATAATGAGCTTACCTTTAAAGCTG
  GAGAAATCATTACAGTCCTTGATGACAGTGATCCCAACTGGTGGAAAGGTGAAACCCATCAAGGCGTGGG
  GCTTTTTCCTTCTAATTTTGTAACTGCAGATCTTACTGCTGAACCAGAAATGATTAAAACAGAGAAGAAG
  ACGGTACAGTTCAATGATGATGTTCAGATAGAGACAATAGAACCAGAACCAGAACCAGCCTTTATTGATG
  AGGATAAAATGGACCAGTTGTTACAGATGTTGCAAAGTACAGATCCTAGTGATAATCAGCCAGACTTGCC
  AGAGCTGCTTCATCTTGAAGCAATGTGTCACCAGATGGGACCTCTCATTGATGAAAAGCTGGAAGATATT
  GATAGAAAACATTCAGAACTGTCAGAACTAAACGTGAAGGTGATGGAAGCTCTCTCACTGTATACGAAGT
  TAATGAATGAAGACCCAATGTACTCTATGTATGCAAAATTACAGAGTCAGCAGTACTATCTGCAGTCGTC
  GGCTGTCTCTGCTTCCCAGGTATATCCAGGGCCTGCGCAGAGCGGTACCTACCTGGTTGCAGGGAGTGCA
  CAGATGACCCATCTGCAGAGCTACAGCCTTCCCCCAGAGCAGCTGTCTTCTATCAGCCAAGGAGCAGTTC
  CATCCTCTGCAAATCAAGCTCTTCCTAGTCAGCAGACCCAGGCTTCTTACCCGAATGCAATGGTTAGTTC
  TGTTCAAGGAAATTCGTATCCTAGCCAGGCTTCAATATATAGTCCTCCTGCTGCTGCTGCTGCTGCCGCT
  GCTGCTGCTGTAGTACCTGTTCCTGTACCTGCTGATGTCACCATATACCAGAATGCAGGACCTACTATGT
  CCCAGGTGCCAAACTATACTTTAACATCATCAACCCTGCCTCAAACAGGAGGCAGCCAACAGCCACCTCA
  GCCACAGCAAGCATATTCTCAGAAGGCTCTGCTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011484
• Insert Size: 1647 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011484.2, NP_035614.1
• RefSeq Size: 3720 bp
• RefSeq ORF: 1647 bp
• Locus ID: 20844
• UniProt ID: P70297
• Cytogenetics: 2 A1-A2
• Gene Summary: Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).