Rapgef5 (BC046627) Mouse Untagged Clone

CAT#: MC218582

Rapgef5 (untagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 5 (cDNA clone MGC:54925 IMAGE:6494175), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rapgef5
• Synonyms: mmr-gef, mKIAA0277, 4932413M22, GFR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC046627
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGCTCCCGCTTGCGGGTCTTTGACCCCCCTCTAGAGAGGAAAGATTCCGCGGCGCTCTCAGAGC
  GCCAGCTGCCCTTGCCTACCTTCGATGTGCCTTATTTCAAATACATCGACGAAGAAGATGAGGACGATGA
  ATGGAGCAGCCGCTCACAGTCTTCCACGGAGGATGACTCGGTGGACTCTCTGCTCTCTGACAGATATGTG
  GTGGTGTCTGGTACCCCGGAGAAGATCTTGGAGCACCTTTTGAATGACTTGCACCTGGCAGAGGTTCAGC
  ACAAAGAAACAGAAACACTCCTTGATGACTTCCTCCTCACGTACACCGTATTCATGACAACTGATGACTT
  GTGCCAGGCGCTGCTAAGACACTATTCTGCCAAGAAGTATCAAGGGGAAGAAGAAAACTCAGATGTCCCT
  TGTCGGAAGCGCAAGGTCTTACATCTGGTTTCCCAGTGGATTTCTCTGTACAAGGACTGGCTACATGAAG
  ATGAGCATTCAAAAATGTTTCTAAAGACCATATACAGGAACGTGCTGGATGATGTCTATGAGTACCCGAT
  TCTTGAGAAAGAGCTGAAAGAGTTTCAGAAGATACTTGGCGTGTACCGCCGTCACACTGTTGATGAATAC
  TCACCACAGAAGAAGAATAAAGCCCTTTTCCACCAATTCAGTCTTAAGGAGAACTGGCTCCAGCACAGAG
  GAACTGTGGCAGAAACAGAGGAAATTTTTTGCCACGTGTATATAACGGAGCACTCCTATATCAGTGTGAA
  GGCAAAAGTGTCGAGTACAGCCCAGGAGATCCTGAAGGTCGTGGCCGAGAAGCTGCAGCGTGCCAAAGAG
  GATCTGGCTCTGGTAGCCATTACATTCTCTGGAGAAAAGCATGAATTTCAGCCAAATGACTTGGCCATTT
  CCAAATCCCTGGAGGCATCTGGTCGGATATATGTCTACCGAAAAGATCTGGCTGACACTTTGAATCCTTT
  GGCAGAAAATGAGGAATCCCAGCAAAGGTCAATGAGGATTTTGGGGATGAACACGTGGGACCTTGCTCTG
  GAATTAATGAGTTTTGATTGGAGTCTGTTCAATTCAATCCACGAGCAAGAGCTGATCTACTTCACATTCA
  GCAGACAGGGGAACGGAGAGAATACTGTGAACCTCAGCCTTCTGCTCCAGAGATGTAACGAGGTCCAGCT
  CTGGGTAGCCACAGAGATTCTGCTCTGCAGCCAGCTAGGCAAGCGCGTGCAGCTGGTGAAAAAATTCATC
  AAGATTGCAGCTCACTGCAAAGCCCAACAGAACCTAAACTCTTTCTTTGCCATCGTGATGGGTCTCAACA
  CCGCCTCTGTCAGCCGGCTATCACAGACCTGGGAGAAAATCCCTGGGAAGTTTAAGAAGCTTTTCTCTGA
  ACTTGAGAGTTTAACGGATCCTTCCCTAAACCACAAAGCCTACAGGGATGCGTTCAAAAAGATGAAGCCA
  CCAAAAATCCCTTTCATGCCCTTACTGCTTAAAGATGTAACGTTTATTCATGAAGGAAATAAAACATTCT
  TGGATAATCTCGTCAATTTTGAAAAGCTGCACATGATTGCAGACACTGTCCGAACCCTGAGACACTGCAG
  GACTAACCAGTTTGGAAGTGACGTGTCTCCAAAGGAGCAGCAAGAGCTGAAGTCCTATGTCAACCACCTG
  TATGTCATCGACAGCCAGCAAGCCCTGTTCGAGCTCTCTCACAGGCTGGAGCCTCGAGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC046627
• Insert Size: 1743 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC046627, AAH46627
• RefSeq Size: 2954 bp
• RefSeq ORF: 1742 bp
• Locus ID: 217944
• Cytogenetics: 12 F2
• Gene Summary: Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation (By similarity).[UniProtKB/Swiss-Prot Function]