Fanci (BC027836) Mouse Untagged Clone

CAT#: MC218552

Fanci (untagged) - Mouse Fanconi anemia, complementation group I (cDNA clone IMAGE:5322726), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fanci
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC027836 edited
  CCACGCGTCCGAGAGCGGGAATTTTGGAAGGATCCCGAGCTGAGGTTGAGTTCTCTGATATGAGCAACCA
  TGGATCTGAAAATTTTATCTCTAGCAACAGATAAAACTACAGATAAACTGCAGGAGTTTCTTCAAACCCT
  GAAAGATGATGATTTGGCCAGTCTCCTTCAGAACCAAGCGGTGAAAGGAAGAGCTGTTGGAACGCTCCTG
  AGAGCCGTCCTCAAAGGCTCCCCCTGCTCTGAGGAAGATGGAGCCCTTAGGAGATACAAGATATATTCTT
  GCTGTATCCAGTTGGTTGAATCGGGAGATTTGCAGCAAGATGTCGCATCAGAGATCATAGGATTACTAAT
  GCTGGAGGTCCACCATTTTCCAGGCCCATTACTTGTTGACTTGGCTAGTGACTTTGTGGGTGCTGTCCGA
  GAGGACCGCCTAGTGAATGGAAAATCTTTGGAGTTATTACCCATCATCCTTACTGCCTTGGCCACCAAGA
  AGGAAGTCCTCGCTTGTGGGAAAGGTGATTTGAATGGGGAAGAATATAAGAGGCAGTTGATTGACACCCT
  GTGTTCTGTCAGATGGCCTCAACGGTACATGATCCAGCTCACTTCTGTGTTCAAGGATGTCTGTCTAACG
  CCAGAAGAGATGAATCTCGTGGTGGCCAAAGTGTTGACGATGTTCTCAAAGTTGAATCTTCAAGAAATTC
  CACCTTTGGTCTATCAGCTTCTGGTTCTCTCTTCCAAGGGAAGCAGAAGGAGTGTTCTGGACGGAATCAT
  AGCCTTCTTCAGGGAGCTGGATAAGCAGCACAGGGAGGAACAGAGCAGCGATGAGTTGTCAGAGCTTATT
  ACTGCCCCAGCAGACGAACTCTATCACGTGGAAGGCACCGTTATTCTGCACATCGTATTTGCCATCAAAC
  TGGACTGTGAATTAGGCAGAGAACTTCTGAAACACTTGAAGGCTGGACAGCAGGGGGATCCCAGTAAATG
  CCTATGTCCCTTCAGCATCGCTCTTCTCCTCTCTCTCACGAGAATACAGAGATTTGAGGAACAGGTGTTT
  GATCTTTTAAAGACTTCTGTTGTAAAAAGCTTCAAGGATCTTCAGCTCCTCCAAGGCTCCAAGTTTCTTC
  AGACTCTCGTCCCCCAGAGAACTTGTGTTTCAACCATGATCTTAGAAGTGGTGAGGAACAGACTTGTTTT
  CAGATATCATCATGTATGCACCTTTAATTCTTCAGAACTGTTCAAAAGTCACGGAAACTTTTGACTATTT
  GACCTTTCTGCCCCTCCAGACCGTGCAAGGGCTGCTCAAGGCGGTGCAGCCGCTTCTCAAGATCAGCATG
  TCGATGAGAGACTCCTTGATCCTCGTCTTGCGGAAAGCTATGTTTGCCAGCCAGCTGGATGCCCGGAAAT
  CTGCAGTTGCTGGGTTTTTGCTGCTCCTAAAGAACTTTAAAGTCCTAGGCAGCCTGCCGTCCTCACAGTG
  CACACAGTCTATTGGTGTCACGCAGGTGCGCGTGGACGTTCACAGCCGATACAGCGCTGTCGCCAACGAG
  ACCTTCTGCCTTGAGATCATAGACAGCTTGAAACGAAGCTTAGGGCAGCAGGCAGACATCCGGCTCATGC
  TTTACGACGGTTTCTACGATGTCCTTCGACGGAACTCTCAGCTGGCTAGTTCAATCATGCAGACTCTCTT
  CTCACAGTTAAAACAGTTCTATGAGCCAGAGCCTGATCTGCTACCTCCTCTGAAACTAGGAGCTTGTGTT
  CTGACCCAAGGAAGTCAGATCTTTCTACAAGAGCCGCTGGATCATCTGCTGTCTTGCATTCAGCATTGCT
  TGGCCTGGTATAAGAGCAGAGTCGTCCCCCTGCAACAAGGAGATGAAGGAGAGGAGGAGGAGGAGGAGTT
  GTACTCCGAGCTGGATGATATGTTGGAGTCCATTACTGTCAGAATGATCAAGAGTGAGCTGGAAGACTTT
  GAGCTGGATAAATCAGCAGATTTCTCTCAGAACACTAATGTCGGCATCAAGAATAATATCTGTGCGTGTC
  TCATCATGGGAGTGTGTGAAGTTCTAATGGAATATAATTTCTCCATAAGTAACTTCAGCAAGAGTAAGTT
  TGAGGAGATCCTGAGCCTCTTCACATGTTACAAGAAGTTCTCTGACATCCTCAGTGAGAAAGCTGGCAAA
  GGCAAGGCTAAGATGACCAGTAAAGTCAGCGACAGTCTCCTGTCCTTGAAGTTTGTGTCTGATCTCCTCA
  CTGCTCTCTTCAGAGACAGCATCCAGAGTCATGAAGAGAGTCTGTCTGTCCTGCGGTCCAGCGGCGAGTT
  TATGCACTATGCAGTGAACGTCGCTTTGCAGAAGATTCAGCAGCTAATACGAACAGGGCATGTGAGTGGC
  CCTGATGGTCAGAACCCAGACAAGATCTTTCAGAACCTCTGTGACATAACTCGGGTCCTGCTGTGGAGAT
  ACACATCAATTCCTACATCCGTGGAGGAGTCGGGGAAGAAGGAGAAGGGAAAGAGCATCTCGCTGTTGTG
  CTTGGAGGGCTTGCAGAAGACGTTCAGTGTGGTGCTGCAGTTCTACCAGCCCAAGGTCCAGCAGTTTCTC
  CAGGCTCTGGATGTCATGGGCACAGAAGAAGAAGAAGCAGGTGTCACTGTCACTCAGAGGGCCTCGTTCC
  AGATCCGGCAGTTTCAGAGGTCGTTGTTGAATCTGCTGAGCAGCGAAGAGGACGATTTTAACAGTAAAGA
  GGCCCTCCTGCTCATCGCGGTGCTGTCCACCTTGTCCAGGCTGCTGGAGCCCACCTCTCCTCAGTTTGTG
  CAGATGTTATCCTGGACATCTAAGATTTGCAAGGAATACAGCCAGGAGGATGCCTCATTTTGCAAGAGTT
  TGATGAACCTGTTCTTCAGCCTGCATGTCTTATATAAGAGTCCTGTCACCCTGCTTCGTGACTTGTCCCA
  GGATATCCATGGGCAGCTGGGAGACATAGATCAAGATGTAGAGATTGAGAAGACAGACCACTTTGCTGTG
  GTGAATTTGAGAACGGCTGCCCCCACTGTCTGTTTACTTGTTCTGAGTCAGGCTGAGAAAGTCCTAGAAG
  AAGTGGACTGGTTAATCGCCAAGATCAAGGGCTCAGCAAACCAAGAAACGCTATCAGATAAAGTGACTCC
  AGAAGATGCCTCTTCTCAAGCCGTTCCACCAACTCTTCTCATTGAGAAAGCTATCGTCATGCAACTGGGA
  ACTCTGGTAACGTTCTTCCATGAGCTCGTGCAGACAGCCCTGCCATCAGGCAGCTGTGTAGACACTCTAC
  TGAAGGGCCTGTCCAAGATCTACAGCACCCTAACAGCATTCGTCAAATATTATCTCCAGGTATGTCAGAG
  CTCCAGAGGAATTCCAAACACTGTGGAAAAACTGGTGAAGCTGTCGGGCTCTCATCTGACCCCCGTATGT
  TACTCTTTCATTTCTTATGTACAAAATAAGAGCAGTGACGCCCCAAAGTGCTCAGAAAAGGAGAAGGCTG
  CAGTGAGCACAACCATGGCCAAAGTTCTTCGTGAAACCAAGCCAATCCCTAACCTTGTCTTTGCCATTGA
  ACAGTATGAAAAATTTCTTATCCAACTGTCTAAAAAGTCCAAGGTAAAAATGTTCTTTATTTCCACTCTC
  ATTTACTGCTGGAAGGAGTCTAGCAAGCTCTGTGGCAGGCAGCACAGCTAGAGGTGGTCAAGGTGAATGT
  CATCTGGTAAAAGCAAAGGGTCCTCCAAGTACACACTGAAAGGACCTTCTGCTGTCCCAGGTAAACCTGA
  TGCAGCACATGAAGCTGAGTACTTCTCGGGACTTCAAGATCAAAGGCAGTGTCCTCGACATGGTTCTTCG
  AGAGGATGAGGAAGACGAAAACGAAGAGGGCACTGCATCAGCACATACACAGCAGGACAGAGAACCAGCC
  AAGAAGAGGAGGAAGAAATGCCTGAGTTAATGTGACCTTTGGGGCATCTGCTTCATTTGTGCCCAACAGT
  ACCCCTTGTCACACAGCCTGCACTGATGTTGGTGTCCTGGTTCTGAACGCACTGAATTGTGCCTTCCGTT
  AGGAGGACTTTCCTGGGCAGGTCCTGCTACAGAAAAATGGCTGCCTTTTGCAAAAAAGCACATCTGAAAG
  CCTGAGTTTAGGAACCTGCACCGCTCCAATAAAGCTCCACAGGAGCAAATACAGAACCTCCAGACAGAGC
  TAGGGTCCAGGCTGGCTTCGTTTTTCCAAGGAGCCTTTGGTGAGTTCAATTATCTGGTAAATATCCAGTG
  CTTCACCTGTAAGACGGTGGAAATTGGTTAGGATGCCTCCAGAATTGTGACTGAACTCAGAAATGAGAAT
  CTGAATGTTGGGGTCAAAGACAGTGGGTGTGAGGGAGTCTTTGAGAGTGGCAGCTTGTCTTATTATAACA
  CAAGCTTTCTAACCAACTCTGGCATTTTCAGCACAGATACATTTTTTCCACATAAGAATGCACTTTGGCT
  
• Restriction Sites: SgfI-MluI
• ACCN: BC027836
• Insert Size: 1728 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027836.1
• RefSeq Size: 4524 bp
• RefSeq ORF: 1184 bp
• Locus ID: 208836
• Cytogenetics: 7 D2
• Gene Summary: Plays an essential role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA: binds both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage.[UniProtKB/Swiss-Prot Function]