Ttc19 (BC037111) Mouse Untagged Clone

CAT#: MC218455

Ttc19 (untagged) - Mouse tetratricopeptide repeat domain 19 (cDNA clone MGC:47198 IMAGE:5370464), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ttc19
• Synonyms: 2010204O13Rik; 2810460C24Rik; AI505442
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC037111
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGACGAGCCAGAAGCGGCCGAGCTGATTTTGCATGACGCTCTTCGCCTTGCGTATGAGAGTGACA
  ACAGGAAGGCCATCACGTACACTTATGACCTGATGGCCAACTTAGCATTTATACGAGGGCAGCTTGAAAA
  TGCAGAACAGCTTTTTAAAGCAACGATGAGTTATCTGCTTGGAGGAGGCATGAAGCAGGAAGACAATGCA
  ATAATTGAAATCTCTCTGAAACTGGCCAACATCTATGCTGCTCAGAATAAACAGGAATTTGCCCTTGCTG
  GCTATGAATTCTGCATTTCAACTCTAGAGGGGAAAATTGAAAGAGAAAAGGAATTAGCAGAAGATATTAT
  GTCAGAAGAAACAGCCAATACTTACCTCCTCCTTGGTATGTGCTTAGACTCTTGTGCACGCTATCTGCTA
  TTCTCCAAGCAGCTATCACAGGCACAAAGGATGTATGAAAAAGCCCTGCAGATTTGTCAAGAAATACAAG
  GAGAAAGACACCCACAGACCATTGTGCTGATGAGTGACTTGGCTACTACTCTGGATGCACAGGGCCACTT
  TGATGATGCCTACATTTATATGCAGAGGGCGTCAGATCTGGCAAGAGAGATAAATCACCCTGAGCTGCAC
  ATGGTACTCAGCAATCTGGCTGCCATTTTGATACACAGAGAGCGATACACACAAGCAAAGGAGATCTACC
  AGGAAGCGCTGAAGCGAGCAGAGCTGAAAAGAGATGAGGTTTCTGTTCAGCACATCAGGGAAGAATTGGC
  TGAACTGTCAAGAAAAAGTAGACGTTTGACTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC037111
• Insert Size: 804 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC037111, AAH37111
• RefSeq Size: 2548 bp
• RefSeq ORF: 803 bp
• Locus ID: 72795
• Cytogenetics: 11 B2
• Gene Summary: Required for the preservation of the structural and functional integrity of mitochondrial respiratory complex III by allowing the physiological turnover of the Rieske protein UQCRFS1 (PubMed:21278747, PubMed:28673544). Involved in the clearance of UQCRFS1 N-terminal fragments, which are produced upon incorporation into the complex III and whose presence is detrimental for its catalytic activity (PubMed:28673544).[UniProtKB/Swiss-Prot Function]