Rshl2a (BC110284) Mouse Untagged Clone

CAT#: MC218366

Rshl2a (untagged) - Mouse radial spokehead-like 2A (cDNA clone MGC:117841 IMAGE:5150198), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rshl2a
• Synonyms: Rshl2b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC110284
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCACCAACATTTGGGCGGCCCTGCCTGCCAAGAAGCGGCCACTGCACCAGCGCGCCCGACGAC
  CAGCAGGTGGGCGGGGCCGCGAGCCGGAAGTGCCCTTTACTACAGACCCTTCGGGAAACCCAGCTGGCCG
  GAACTGCCTGGAGTTCCTGCCTCCAGGCGGAACCTCGGGTTGCTCGGCAACCGATGGGGGCGCAACTGAC
  TCCCTCCACGTTCCCGAGAGAAACCTGAGGGTACCAGCAACCTGCTGCTGGGGCCCCCTCGCGTCCCGCC
  CGCTTCCCCGAGAGCGGCGTCCTCTCGGGTCGCAGCATCCCTGCCCTTGGCACTACTTACAAGTCTCCGA
  CTACGACGACTCCTTAGCCCCAACCTGCTTCAGAGCCCACCTTCACCGGAGGGGAAGCAGTTCGACGCTC
  AACAAGGCTTCGGCGATGACAGACCGTAACCCTAGGACAGCCGAGGCCTCAGGCCTCTACACCTATTCCA
  GCAGGCCCCGGGCCGTGGCTTGCCAGCGCCGCCGCTACGGAGACAGCATTTTGCAGCCAGTTGAAGAACC
  TATGAGTTACGGGAACATCATGTATGACAGAAGGGTGATCCGAGGCAACACCTATGCACTGCCCACAGGA
  CAGGTGCCTGGACAGCCTGATCCTCTAGAGCTTCAGCGACAGCAGCAGGCCAGGAGAAGGGCTCTTGCCA
  GAAAACGAGCCCAGGAGCAGCTCAAACCACGAACGCCTGAGCCCGTGGAGGGCAGAAAGCATGTGGACAT
  ACAGACAGAATTGTACCTTGAAGAAATTGCTGATCGCATAGTTGAAGTCGACATGGAATGCCAGACAGAT
  GCATTTCTGGACAGACCACCCACACCCCTCTTCATTCCTGCCAAAACTGGCAAAGATGTGGCCACTCAGA
  TCCTGGGAGGAGAGCTTTTTGACTTTGACCTTGAAGTCAAGCCCATGTTAGAGGTTTTGGTGGGGAAGAC
  GATTGAACAGTCCCTTCTAGAGGTGATGGAAGAGGAGGAGCTGGCCAACCTGCGGGCCAGGCAGTATGCA
  TATGAAGAGATAAGGAACGTGGAGCTGGCCGAAGTGCAGCGACTGGAAGAGCAGGAGAGAAGGCACCGAG
  AAGAAAAGGAGCGCCGTAAAAAACAGCAGTGGGAGATTGTCCATAAGCAGAATGAGACGTCACAGAAGAT
  CTCAGCTCGGGCATTCACTCAGCAGTACCTGGCTGACCTCCTCCCATCTGTCTTTGACAGTCTCAGGAAT
  AGTGGCTACTTTTATGATCCAATTGAAAGAGATATTGAGGTAGGATTCCTCCCATGGCTAATGAATGAAG
  TTGAAAAATCCATGGAACACAGCATGGTGGGAAGAACTGTGCTTGACATGTTGATTCGTGAAGTGGTTGA
  AAGAAGGATCAACGATTACGAGCATAAGGAAGCCATGCTGCCTGCTCAGAAGACAAATGATGTGATTAAT
  GGTCCTAACACAGTGACAGATCCCTCGGTGAGTTTAGAATTACAGGAGTCAACCACATTGCACATGCAGA
  AGCCAGTTTTAGACAGAGTCTCCTCACAGCCAGCACCATCCCAAGAAAGGAAGCCTGTGGAAGAGGAGGA
  CACCTTATGGCAGAGTGACCAATCAGAACTGAGGGAGTCTGTAGGGGAGGAACTGTCACAACCAGAAGGC
  TCACTGGAGCCAGAGAGCAGCTCAACATCTGGTCAGTTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC110284
• Insert Size: 1722 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC110284, AAI10285
• RefSeq Size: 1972 bp
• RefSeq ORF: 1721 bp
• Locus ID: 66832
• Cytogenetics: 17 A1
• Gene Summary: Functions as a protein kinase A-anchoring protein that scaffolds the cAMP-dependent protein kinase holoenzyme. May serve as a point of convergence for MAPK and PKA signaling in cilia (By similarity).[UniProtKB/Swiss-Prot Function]