Bmp8a (BC052168) Mouse Untagged Clone

CAT#: MC218141

Bmp8a (untagged) - Mouse bone morphogenetic protein 8a (cDNA clone MGC:59383 IMAGE:6334507), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bmp8a
• Synonyms: OP2, OP-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052168
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTATGCGTCCCGGGCCACTCTGGCTATTGGGCCTTGCTCTGTGCGCGCTGGGAGGCGGCCACGGTC
  CGCGTCCCCCGCACACCTGTCCCCAGCGTCGCCTGGGAGCGCGCGAGCGCCGCGACATGCAGCGTGAAAT
  CCTGGCGGTGCTCGGGCTACCGGGACGGCCCCGACCCCGTGCACAACCCGCCGCTGCCCGGCAGCCAGCG
  TCCGCGCCCCTCTTCATGTTGGACCTATACCACGCCATGACCGATGACGACGACGGCGGGCCACCACAGG
  CTCACTTAGGCCGTGCCGACCTGGTCATGAGCTTCGTCAACATGGTGGAACGCGACCGTACCCTGGGCTA
  CCAGGAGCCACACTGGAAGGAATTCCACTTTGACCTAACCCAGATCCCTGCTGGGGAGGCTGTCACAGCT
  GCTGAGTTCCGGATCTACAAAGAACCCAGCACCCACCCGCTCAACACAACCCTCCACATCAGCATGTTCG
  AAGTGGTCCAAGAGCACTCCAACAGGGAGTCTGACTTGTTCTTTTTGGATCTTCAGACGCTCCGATCTGG
  GGACGAGGGCTGGCTGGTGCTGGACATCACAGCAGCCAGTGACCGATGGCTGCTGAACCATCACAAGGAC
  CTGGGACTCCGCCTCTATGTGGAAACCGCGGATGGGCACAGCATGGATCCTGGCCTGGCTGGTCTGCTTG
  GACGACAAGCACCACGCTCCAGACAGCCTTTCATGGTAACCTTCTTCAGGGCCAGCCAGAGTCCTGTGCG
  GGCCCCTCGGGCAGCGAGACCACTGAAGAGGAGGCAGCCAAAGAAAACGAACGAGCTTCCGCACCCCAAC
  AAACTCCCAGGGATCTTTGATGATGGCCACGGTTCCCGCGGCAGAGAGGTTTGCCGCAGGCATGAGCTCT
  ACGTCAGCTTCCGTGACCTTGGCTGGCTGGACTGGGTCATCGCCCCCCAGGGCTACTCTGCCTATTACTG
  TGAGGGGGAGTGTGCTTTCCCACTGGACTCCTGTATGAACGCCACCAACCATGCCATCTTGCAGTCTCTG
  GTCAGTACCACAGTAGCCTGCTGCGACAGGTGGAGCGGGGTGCACCTGATGAAGCCAGATGTTGTCCCCA
  AGGCATGCTGTGCACCCACCAAACTGAGTGCCACCTCTGTGCTGTACTATGACAGCAGCAACAATGTCAT
  CCTGCGTAAACACCGTAACATGGTGGTCAAGGCCTGTGGCTGCCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC052168
• Insert Size: 1239 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052168, AAH52168
• RefSeq Size: 2412 bp
• RefSeq ORF: 1238 bp
• Locus ID: 12163
• Cytogenetics: 4 57.42 cM
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein may play a role in development of the reproductive system. Mice lacking a functional copy of this gene exhibit degeneration of germ cells and the epididymal epithelium. This gene may have arose from a gene duplication event and its gene duplicate is also present on chromosome 4. [provided by RefSeq, Jul 2016]