5730409G15Rik (BC051078) Mouse Untagged Clone

CAT#: MC217870

5730409G15Rik (untagged) - Mouse RIKEN cDNA 5730409G15 gene (cDNA clone MGC:59002 IMAGE:6311572), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 5730409G15Rik
• Synonyms: 5730409G15Rik; eEF2-KMT; Fam86; Fam86a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC051078
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCAAGAGTCCACCCAGTGCCACCGGAGCTATTTGCTGCCTTCAGGAAACTCAGTTACACTCTCTG
  AGAGCACTGCCATCGTGTCCCATGGCACCACAGGCTTGGTTACATGGGACGCTGCTCTCTACCTGGCAGA
  ATGGGCCATTGAGAACCCAGCAGCCTTCACTGACAGGACTATCCTGGAGCTAGGCAGTGGAGCTGGCCTT
  ACAGGCCTGGCAATTTGCAAGGCATGCTGCCCCAGAGCATACATCTTCAGCGACTGTCATGCCCAAGTGC
  TTGAGCAGCTCCGTGGAAACGTTCTCCTCAACGGCTTCTCCTTAGAGCCGCACACCCCCATTGACGCAGG
  CAGCTCGAAGGTGACAGTGGCTCAGCTGGACTGGGACGAGGTGACAGCCTCTCAGCTCTCTGCCTTCCAG
  GCAGATGTTGTCATTGCAGCAGATGTACTGTACTGCTGGGAGATGACACTGTCACTGGTCAGGGTCCTGA
  AGATGCTCGAGGACTGCCAGAGGAAGAGCGCTCCTGATGTCTATGTAGCCTATACTATCCGCAGCCAGGA
  CACAGGCAAGCTGTTCATCGAAGAGCTGGACCGTGCTGGAATCTACTGGGAAGAGGTGCCCCCTCACACT
  GGCAAACTGTTCCCTTACGAAGAGCATTCAGCCATCGTAATCCTGAAGCTGGTGCTGACCAGTCGCCATG
  GTGTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC051078
• Insert Size: 708 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC051078, AAH51078
• RefSeq Size: 1560 bp
• RefSeq ORF: 707 bp
• Locus ID: 70511
• Cytogenetics: 16 2.51 cM
• Gene Summary: Catalyzes the trimethylation of eukaryotic elongation factor 2 (EEF2) on 'Lys-525'.[UniProtKB/Swiss-Prot Function]