Cerk (NM_145475) Mouse Untagged Clone

CAT#: MC217643

Cerk (untagged) - Mouse ceramide kinase (Cerk), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cerk
• Synonyms: AI848610; D330016D08Rik; mKIAA1646
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217643 representing NM_145475
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGCAATGGGGGCGGCGGAGCCGCTGCACTCCGTGCTGTGGGTGAAACGGCGACGCTGTGCCGTCA
  GCCTGGAGCCCGCGCGGGCTCTGCTACGCTGGTGGCGGAGCCCGGAGCCCGGGCCCTCCGCGCCGGGTGC
  CGATGCCCGCTCGGTACTGGTGTCGGAGATCATCGCTGTTGAGGAAAAAGACGACTGCGAGAAACACGCC
  TCCAGTGGCCGATGGCATAAAATGGAAAATCCGTTCGCATTCACAGTCCACCGTGTGAAGCGAGTACGAC
  ATCACCGCTGGAAGTGGGCACGGGTGACCTTCTGGAGCGCCGACGAGCAGCTGTGTCACCTGTGGCTGCA
  GACCCTCCGTGGGCTGCTGGAGAGCCTGACTTCAAGACCGAAGCACTTGCTGGTATTCATCAACCCTTTC
  GGAGGGAAAGGTCAGGGCAAGCGCATCTATGAAAAAACAGTGGCGCCTCTGTTTACCTTGGCTTCCATCA
  CTACGGAGATCATCATTACAGAGCATGCCAACCAAGCCAAGGAGACTTTATACGAGATCAACACAGACAG
  CTATGATGGCATCGTGTGCGTAGGTGGGGACGGCATGTTCAGCGAGGTGCTGCATGGGGTGATTGGGAGG
  ACGCAGCAGAGCGCTGGTATCGACCCCAATCACCCCCGAGCCGTGCTGGTGCCCAGTACCCTCAGGATCG
  GCATCATACCCGCAGGGTCCACAGATTGTGTGTGTTACTCAACAGTGGGCACAAACGACGCAGAGACATC
  GGCTTTGCACATCATTATTGGGGACTCACTGGCAATAGACGTGTCCTCTGTGCACTACCATAACACGCTG
  CTGCGGTACTCGGTTTCTCTGCTGGGCTACGGTTTCTACGGGGACTTAATCAAGGACAGTGAAAAGAAAC
  GGTGGATGGGCCTCGTCCGGTATGATTTCTCAGGGTTGAAGACCTTTCTCTCTCATCAGTACTATGAAGG
  GACACTGTCCTTCCTCCCAGCACAGCACACGGTGGGATCTCCACGGGACAATAAACCCTGCCGGGCTGGG
  TGCTTCGTGTGCAGGCAGAGCAAGCAACAGCTGGAAGAAGAAGAGAAGAAAGCCCTGTATGGCCTGGAGA
  ACGCCGAGGAAATGGAAGAGTGGCAAGTGACATGTGGGAAGTTCCTGGCCATCAATGCCACCAACATGTC
  CTGTGCTTGTCCTCGGAGCCCTGGGGGCCTGTCCCCATTTGCCCATCTGGGAGATGGGTCTTCTGACCTC
  ATCCTTATCCGGAAGTGCTCCAGGTTCAACTTCCTGAGATTCCTCATCCGGCACACGAACCAGGAGGACC
  AGTTCGACTTCACTTTCGTTGAAGTTTATCGAGTCAAGAAATTCCACTTCACGTCGAAGCACGTGGAAGA
  CGAGGACAATGACTCGAAGGAACAAGAGAAGCAGAAGTTTGGGAAGATCTGCAAGGACAGACCCTCTTGC
  ACTTGCTCAGCCTCCAGAAGCTCCTGGAACTGCGATGGCGAAGTCATGCACAGCCCGGCCATTGAGGTCA
  GGGTCCACTGCCAGCTGGTGCGCCTCTTTGCTCGGGGAATCGAGGAAGAGTCATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145475
• Insert Size: 1596 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145475.4, NP_663450.3
• RefSeq Size: 4562 bp
• RefSeq ORF: 1596 bp
• Locus ID: 223753
• UniProt ID: Q8K4Q7
• Cytogenetics: 15 E2
• Gene Summary: Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate (PubMed:16269826). Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines (PubMed:16269826). Shows a greater preference for D-erythro isomer of ceramides (By similarity). Binds phosphoinositides (By similarity).[UniProtKB/Swiss-Prot Function]