Sppl2a (NM_023220) Mouse Untagged Clone

CAT#: MC217462

Sppl2a (untagged) - Mouse RIKEN cDNA 2010106G01 gene (2010106G01Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sppl2a
• Synonyms: 2010106G01Rik; C130089K23Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217462 representing NM_023220
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTGCTGCACTCGCTGCACGCTCCCGCGGCTGCCCTGCTCTGGAGCTGCCTGCTCGGACTGGCAG
  CTGCTCAGGAAGCCATTCTGCATGCGTCTACAAATGGCGTGTCTTCACTTTCCAAGGACTACTGCATGTA
  TTATAACAACAACTGGACACGACTTCCAAGTTCCCTAGAAAATGCAACTTCTCTGAGCTTGATGAATCTG
  ACGGGCACAGCACTGTGCCACCTTTCTGATATTCCTCCTGATGGCATAAGGAACAAGGCAGTGGTGGTGC
  ACTGGGGACCCTGCCATTTTCTTGAAAAAGCCAGAATTGCACAGGAAGGTGGTGCTGCAGCATTGCTGAT
  TGCCAACAACAGTGTCCTAATTCCTTCCTCAAGGAACAAATCTACATTTCAAAATGTGACTGTACTAATT
  GCAGTTATAACCCAAAAAGACTTCAAAGACATGAAAGAGACTCTTGGAGATGACATTACCGTGAAAATGT
  ATTCCCCATCCTGGCCTAACTTTGACTATACTCTGGTGGTTATTTTTGTAATTGCTGTGTTCACTGTGGC
  CTTAGGAGGATACTGGAGTGGACTTATTGAATTGGAAAACATGAAGTCAGTGGAAGACGCAGAAGACAGA
  GAGACAAGAAAAAAGAAGGACGATTACTTAACATTCAGTCCTCTCACGGTTGTTGTGTTCGTGGTCATCT
  GCTGTATAATGATTGTCTTACTGTATTTCTTCTACAGGTGGCTTGTGTATGTTATGATAGCGATTTTCTG
  CATAGCATCGTCAATGAGCCTGTACAACTGTCTTTCTGCATTGATTCATAGGATGCCATGTGGACAGTGC
  ACGATTTTGTGTTGTGGCAAAAACATTAAAGTGAGCCTGATTTTCCTTTCCGGGCTGTGCATCTCAGTTG
  CTGTTGTGTGGGCTGTATTTCGAAATGAGGATAGGTGGGCTTGGATTTTACAGGATATCTTAGGAATTGC
  TTTCTGTCTCAACTTAATTAAAACAATGAAGTTACCCAATTTCATGTCATGCGTGATACTGCTAGGCCTT
  CTTCTCATCTACGATGTGTTCTTTGTCTTCATCACACCTTTCATCACAAAGAATGGTGAGAGCATCATGG
  TGGAACTTGCTGCTGGGCCTTTCGAGAATGCTGAAAAGTTGCCAGTAGTTATCAGGGTGCCAAAACTGAT
  GGGTTACTCAGTCATGAGTGTATGCTCCGTGCCTGTTTCAGTATTGGGTTTCGGAGATATCATTGTACCA
  GGCCTGTTGATTGCATATTGTAGAAGATTCGATGTTCAGACTGGTTCTTCTATATACTATATTTCATCCA
  CAATTGCCTATGCTGTTGGCATGATCATTACCTTTGTTGTCCTGATGGTGATGAAAACAGGGCAGCCTGC
  TCTCCTCTACTTGGTACCTTGTACACTTATTACTGTCTCAGTCGTTGCTTGGAGTCGTAAGGAAATGAAA
  AAGTTCTGGAAAGGCAGCAGCTATCAGGTGATGGACCACCTGGACTATTCAACAAATGAAGAAAATCCAG
  TGACGACTGATGAGCAGATTGTACAACAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023220
• Insert Size: 1572 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023220.2, NP_075709.2
• RefSeq Size: 5629 bp
• RefSeq ORF: 1572 bp
• Locus ID: 66552
• UniProt ID: Q9JJF9
• Cytogenetics: 2 F1
• Gene Summary: Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in FASLG, ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. Also responsible for the intramembrane cleavage of Fas antigen ligand FASLG, which promotes the release of the intracellular FasL domain (FasL ICD). May play a role in the regulation of innate and adaptive immunity.[UniProtKB/Swiss-Prot Function]