Magee2 (NM_053206) Mouse Untagged Clone

CAT#: MC217407

Magee2 (untagged) - Mouse melanoma antigen, family E, 2 (Magee2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Magee2
• Synonyms: 9630059J11Rik; BB131182; Mage-e2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217407 representing NM_053206
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTGGTAAGCCAGAATGCGCGCCAAAGCAGCGCAGAGACTACCGCCGATTACAGCAACAGCCAGG
  GTGAAATGCAGGCTACTAATGCCTCCGGGCCTGCCACCTCCATGCTAGCCCTCGAAGCTCCCCAGGGCCC
  TGAGATTCCAAACGATCCACAGGGAGCCAGCGCTTCCCAGGCTGTGCGGGACCCGAGTGACATCGAGGTT
  CTCATCGACGAGCAGTCCCGACGTTTGGGGGCGCTCAGGGTCCACGACCCTCTTGAAGAGAGGTCGGTTG
  CTTTAGTGAATTTCATGCGCATGAAAAGCCAAAATGAGGGGTCTATCCGACAGTCAGAAATGCTGGAGTT
  TCTCAAAGAATACTCAGATCAGTTCCCTGAGATACTCAGACGAGCCTCAGCTCATCTGGATCGGGTCTTT
  GGGTTGAATCTGAGAGTCCTTGATCCTCAGATGGACACTTACAACCTAATCAGCAAACCAGGTCCCCAGA
  CCACTGAACGGCTAGCAGAGACCCTGGACATACCAAAAGCGGGTCTCCTGGCCTTGGTCCTAGGCCATAT
  TCTCTTGAATGGCAACCGGGCAAGAGAGGCCTCCATTTGGGACCTGCTGTTAAAGGTGGATGTGATAGAT
  GGGTCTCAGAGGATCAACAACCAGTTTGGGAACACAAGAAACCTCCTTACTATTGACTTTGTCCATATGC
  GATTTTTGGAGTACTGGCCAGTATATGGCACTAATCCCCTTGAATTTGAGTTCCTGTGGGGCTCTAGAGC
  TCACAGTGAAATCACAAAGATGGAAGCCCTGAAGTTTGTAGCAGAGGCCCATGATGAAGAACCCTGGAGC
  TGGCCAGAAGAATATAACAAAGCTCTGGAAGCTGACAAAGCCAAAGAGAGAAGCCAGGCTGCTGGCTTAG
  AGTTCTGGTCAGAGGACACTATGAATGATAAAGCAAATGAATTGGTCCAGTTGGCCATTGGTGTCACTGA
  GGAGTTGCTGCCTATCCATCAGGATGAGCTATTTGCTAATACTGGCAAAGAATTCCAGGATGTGTTCCCA
  AATATCCTCAGCAGAGCCACTCTAATCCTTGATCTGTTTTATGGGTTCTCTCTGATTGAGGTTGATACCA
  GTGAGCACATCTACCTCCTTGTCCCCCAACCAGAATCAGAAGAAGAACAAGTAATGCTAGAGAGCCTGGG
  CAGACCCACCCAAGAATACTTGATGCCAATCCTGGGTTTGATCTTCCTGATGGGCAACCGTGTCAAAGAG
  GCCAAGATCTGGAACTTACTCCAGAGATTCAGTGTTGATGTAGGGAGGAAACATGCCATCACCTGTAAGC
  TAATGAGGCAACGCTACCTAGAATGCAGGCCGCTCTCCTACTCGAATCCAGTTGAATACGAGCTGCTCTG
  GGGTCCTCGGGCTCACCATGAAATCACCAAAATGAAAGCCTTGGAATATATGGCTAGGTTCTATGGAAAA
  CAGCCACAGGACTGGCCAGAGCAATACAGGGAGGCTGTGGAAGATGAGGAGGCCAGGGCCAGGTCTGAGG
  CAACAGCCATGTTCTTCTTCGGTTCCATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053206
• Insert Size: 1572 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053206.2, NP_444436.1
• RefSeq Size: 2338 bp
• RefSeq ORF: 1572 bp
• Locus ID: 272790
• Cytogenetics: X D