Impdh1 (NM_011829) Mouse Untagged Clone

CAT#: MC217297

Impdh1 (untagged) - Mouse inosine 5'-phosphate dehydrogenase 1 (Impdh1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Impdh1
• Synonyms: B930086D20Rik; IMPDH-I
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217297 representing NM_011829
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACTACCTGATCAGCGGCGGCACCGGCTACGTTCCCGAGGATGGGCTCACCGCGCAGCAGCTCT
  TTGCCAACGCGGATGGCCTCACCTACAACGACTTCCTGATCCTCCCAGGATTCATAGACTTCATAGCTGA
  TGAAGTGGACCTGACATCAGCCCTGACCCGGAAGATCACACTGAAGACACCATTGATCTCGTCTCCCATG
  GATACAGTGACAGAGGCTGATATGGCCATTGCAATGGCTCTCATGGGAGGAATTGGTTTCATTCATCACA
  ACTGTACCCCAGAGTTCCAGGCCAATGAAGTACGGAAGGTCAAGAAGTTTGAGCAAGGCTTCATCACAGA
  CCCTGTGGTCCTGAGCCCTTCACATACTGTGGGTGATGTTCTGGAGGCCAAGATACAGCATGGCTTCTCT
  GGTATCCCCATCACCGCGACGGGCACCATGGGGAGCAAGCTGGTGGGCATCGTCACCTCCCGAGACATTG
  ACTTCCTTGCTGAGAAGGACCACACCACCCTCCTCAGTGAGGTGATGACTCCGAGGGTCGAGCTGGTGGT
  GGCTCCAGCAGGTGTGACATTGAAAGAAGCAAATGAGATCTTGCAGCGCAGCAAGAAAGGGAAGCTGCCC
  ATAGTCAACGATCAAGATGAGCTGGTAGCCATCATTGCGCGCACAGACCTGAAGAAGAACAGAGACTACC
  CTCTGGCCTCCAAGGACTCCCACAAACAGCTGTTGTGTGGGGCAGCTGTGGGCACCCGTGAGGATGACAA
  ATACCGCCTGGACCTGCTCACTCAGGCCGGTGCTGACGTCATAGTACTAGATTCATCCCAGGGGAACTCA
  GTGTATCAGATCGCCATGGTGCACTATATCAAGCAGAAGTACCCCCACCTCCAAGTGATTGGGGGAAATG
  TGGTGACAGCAGCCCAGGCCAAGAACTTGATTGATGCTGGTGTGGACGGGCTTCGTGTGGGCATGGGCTG
  TGGTTCCATCTGCATCACCCAGGAAGTGATGGCCTGTGGCCGACCCCAGGGGACTGCTGTCTACAAGGTG
  GCCGAGTACGCCCGACGTTTTGGGGTGCCGGTAATAGCGGATGGTGGCATCCAGACCGTGGGCCATGTGG
  TCAAAGCCCTGGCACTTGGAGCCTCTACAGTAATGATGGGCTCCCTGCTGGCTGCCACCACGGAGGCGCC
  TGGTGAATACTTCTTCTCAGATGGGGTGAGGCTGAAGAAGTACCGGGGCATGGGTTCTCTGGACGCCATG
  GAGAAGAGCAGCAGCAGCCAGAAAAGATACTTCAGTGAGGGGGATAAGGTGAAGATCGCACAAGGTGTCT
  CCGGTTCCATCCAGGATAAAGGCTCCATTCAGAAGTTTGTGCCCTACCTCATAGCAGGGATCCAGCATGG
  CTGCCAGGATATTGGGGCCCAAAGCCTATCTGTCCTGCGATCCATGATGTACTCAGGAGAGCTCAAGTTT
  GAGAAGCGGACCATGTCGGCCCAGATTGAGGGTGGCGTGCACGGCCTACACTCTTACGAGAAGCGGCTGT
  ACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011829
• Insert Size: 1545 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011829.3, NP_035959.2
• RefSeq Size: 2454 bp
• RefSeq ORF: 1545 bp
• Locus ID: 23917
• UniProt ID: P50096
• Cytogenetics: 6 A3.3
• Gene Summary: Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) has multiple differences, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon, compared to variant 1. The encoded protein (isoform 3) has a shorter N-terminus compared to isoform 1.