Nova1 (NM_021361) Mouse Untagged Clone

CAT#: MC217242

Nova1 (untagged) - Mouse neuro-oncological ventral antigen 1 (Nova1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nova1
• Synonyms: 9430099M15Rik; G630039L02; Nova-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217242 representing NM_021361
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCGGCAGCTCCCATTCAGCAGAACGGGACCCATACGGGGGTTCCCATAGATCTGGACCCGCCGG
  ACTCGCGGAAAAGGCCGCTTGAAGCGCCCCCTGAAGCCGGCAGCACCAAGAGGACCAACACGGGCGAAGA
  CGGCCAGTACTTTCTAAAGGTTCTCATACCTAGTTATGCTGCTGGATCTATAATTGGGAAGGGAGGACAG
  ACAATTGTTCAGTTGCAAAAAGAAACTGGAGCCACTATCAAGCTGTCTAAATCCAAAGATTTTTATCCAG
  GTACTACTGAGAGGGTTTGCTTGATCCAGGGAACAATTGAAGCGCTGAACGCAGTTCATGGATTCATTGC
  AGAAAAAATCCGAGAAATGCCCCAAAATGTTGCCAAGACAGAACCAGTCAGTATCCTACAACCTCAGACC
  ACCGTTAATCCTGATCGCATCAAACAAACATTGCCATCTTCCCCAACTACCACCAAGTCCTCTCCATCTG
  ACCCCATGACCACCTCCAGAGCCAATCAGGTAAAGATTATAGTTCCCAACAGCACAGCAGGTCTGATAAT
  AGGGAAGGGAGGTGCTACTGTGAAGGCTATAATGGAGCAGTCAGGGGCTTGGGTGCAGCTTTCCCAGAAA
  CCCGATGGGATCAACTTGCAAGAGAGGGTTGTCACTGTGAGTGGAGAACCTGAACAAAACCGAAAAGCTG
  TTGAACTTATCATCCAGAAGATACAAGAGGATCCACAGAGTGGCAGCTGTCTCAATATCAGTTATGCCAA
  TGTGACAGGTCCAGTGGCAAATTCCAATCCAACCGGATCTCCTTATGCAAACACTGCTGAAGTGTTACCA
  ACTGCCGCAGCAGCCGCAGGGCTATTAGGACATGCTAACCTTGCTGGCGTTGCGGCCTTTCCAGCAGTTT
  TATCTGGCTTCACAGGCAATGACCTGGTGGCCATCACCTCTGCACTTAATACATTAGCCAGCTATGGATA
  TAATCTCAACACATTAGGTTTAGGTCTCAGCCAAGCAGCAGCAACGGGGGCTTTGGCTGCCGCAGCTGCC
  AGTGCCAACCCAGCAGCAGCAGCAGCCAATTTGTTGGCCACCTATGCCAGTGAAGCCTCAGCCAGCGGCA
  GCACGGCTGGTGGAACGGCGGGGACATTTGCATTAGGTAGCCTGGCTGCTGCTACTGCTGCAACCAATGG
  ATACTTTGGAGCTGCCTCGCCCCTAGCTGCCAGTGCCATTCTAGGGACAGAGAAATCCACAGATGGATCA
  AAGGATGTAGTTGAAATAGCAGTTCCAGAAAACTTAGTTGGTGCAATACTTGGCAAAGGAGGGAAAACCT
  TAGTGGAATACCAGGAGTTGACTGGTGCAAGGATACAGATCTCCAAAAAGGGAGAATTCGTACCTGGCAC
  AAGGAATCGGAAGGTAACCATTACTGGAACACCAGCTGCAACACAGGCTGCTCAGTATTTAATTACACAG
  AGGATCACATATGAGCAAGGAGTTCGGGCTGCCAATCCTCAGAAAGTGGGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021361
• Insert Size: 1524 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021361.1, NP_067336.1
• RefSeq Size: 7000 bp
• RefSeq ORF: 1524 bp
• Locus ID: 664883
• UniProt ID: Q9JKN6
• Cytogenetics: 12 21.46 cM
• Gene Summary: Functions to regulate alternative splicing in neurons by binding pre-mRNA in a sequence-specific manner to activate exon inclusion. It binds specifically to the sequence UCAUY. Most likely acts to activate the inclusion of exon E3A in the glycine receptor alpha-2 chain and of exon E9 in gamma-aminobutyric-acid receptor gamma-2 subunit via a distal downstream UCAU-rich intronic splicing enhancer.[UniProtKB/Swiss-Prot Function]