Ubp1 (NM_013699) Mouse Untagged Clone

CAT#: MC217151

Ubp1 (untagged) - Mouse upstream binding protein 1 (Ubp1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ubp1
• Synonyms: Cp2b; LBP-1a; LBP-1b; NF2d9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217151 representing NM_013699
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGGGTGCTCAGTATGGACGAGGTGATCGAGTCCGGGCTGGTGCACGACTTCGACTCCAGCCTGT
  CCGGCATCGGGCAGGAGCTGGGCGCCGGCGCCTACAGCATGAGTGATGTGTTGGCATTGCCCATTTTCAA
  GCAAGAAGATTCCAGTCTTTCCTTGGAAGATGAGGCCAAGCACCCCCCCTTCCAATATGTAATGTGTGCT
  GCAACTTCGCCAGCCGTTAAGCTGCACGACGAGACACTTACTTACTTGAACCAAGGCCAGTCTTATGAAA
  TTCGGATGCTGGATAATCGCAAAATGGGAGACATGCCTGAGCTCAGTGGGAAGTTGGTAAAGAGCATCAT
  AAGGGTGGTGTTCCATGACAGGCGGCTACAGTACACAGAACATCAGCAGCTTGAAGGCTGGAAGTGGAAT
  CGTCCAGGAGACAGGCTTCTCGATTTAGATATTCCAATGTCTGTGGGAATAATTGACACAAGGACAAATC
  CAAGCCAGTTAAATGCAGTTGAATTTCTGTGGGACCCTGCAAAGCGCACATCTGCCTTCATTCAGGTACA
  TTGTATCAGCACAGAATTCACCCCGCGTAAGCATGGAGGTGAAAAAGGAGTGCCTTTCAGGATCCAGGTT
  GACACCTTTAAGCAGAATGAAAACGGGGAATACACAGATCATCTTCACTCAGCCAGCTGCCAAATCAAAG
  TCTTTAAGCCTAAAGGTGCAGACAGAAAACAAAAAAATGACCGAGAAAAGATGGAGAAGAGAACAGCTCA
  TGAAAAAGAAAAGTACCAGCCATCTTACGACACCACCATTCTCACAGAGTGTTCTCCATGGCCCGACACC
  CCTACAGCCTATGTGAACAACAGCCCTTCCCCAGCGCCCACTTTCACCTCCTCACAACCGAGCACGTGCA
  GTGTCCCAGACAGCAATTCTTCATCCCCAAATCACCAGGGAGATGGAGCTGCACAAGCGTCTGGGGAACA
  AATTCAGCCTTCAGCTACAACCCAGGAAACACAGCAGTGGCTGCTCAAAAACAGGTTCTCTTCCTACACA
  AGACTGTTCTCTAATTTTTCAGGTGCCGACTTATTAAAGCTGACAAAGGAGGATTTAGTTCAGATTTGTG
  GTGCAGCCGATGGAATTCGGCTCTATAATTCCCTGAAGTCAAGGTCGGTTAGGCCCCGCTTAACCATCTA
  TGTCTGCCAGGAGCAGCCGAGTAGCACCGCGCTGCAAGGGCAGCCACAAGCTGCAGGCAGTGGAGGTGAA
  AGTGGCGGTGGGACGCCCTCCGTTTATCATGCAATCTACTTGGAAGAAATGGTTGCCTCAGAAGTTGCTC
  GAAAACTTGCCTCCGTGTTTAATATTCCTTTCCACCAAATTAACCAGGTTTACAGACAGGGCCCCACTGG
  TATCCACATTCTTGTTAGTGACCAGATGGTTCAGAACTTTCAAGATGAAACTTGTTTTTTATTCTCCACA
  GTAAAAGCTGAAAATAATGATGGTATTCACATAATTTTGAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013699
• Insert Size: 1515 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013699.2, NP_038727.1
• RefSeq Size: 3749 bp
• RefSeq ORF: 1515 bp
• Locus ID: 22221
• UniProt ID: Q811S7
• Cytogenetics: 9 F3
• Gene Summary: Functions as a transcriptional activator in a promoter context-dependent manner. Involved in regulation of the alpha-globin gene in erythroid cells. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with TFCP2. Functions as a trans-acting factor that regulates the domestic strain CYP2D9 gene through specific association with the regulatory element SDI-A1. Binding to SDI-A1 depends on the type of nucleotide at position 299; binding is abolished by a nucleotide substitution at this position. Modulates the placental expression of CYP11A1 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an exon in the coding region compared to variant 1. The resulting protein (isoform b) is shorter but has the same N- and C-termini compared to isoform a.