Shb (NM_001033306) Mouse Untagged Clone

CAT#: MC217059

Shb (untagged) - Mouse src homology 2 domain-containing transforming protein B (Shb), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Shb
• Synonyms: BC028832
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217059 representing NM_001033306
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGTGGCTGAACAAGTACTTCAGCTTGGGCAACAGCAAGACTAAGAGTCCGCCGCAGCCGCCGA
  GGCCGGACTACCGGGAGCAGCGGCGCCGGGGCGAGCGACGAGAGCAGCCCCCGCAGGCCGTGCCGCAGGC
  CTGCTCGGCGTCCTCGGCGTCCTGTGGGTCGGCCGCCGCCTGCTTCTCGGCCTCGTCGGGCTCTCTGCCC
  GACGACAGCGGCAGCACGAGTGACCTCATCCGCGCCTACCGCGCGCAGAAGGAGCGCGACTTCGAGGACC
  CGTACAACGGGCCGGGCTCGTCGCTGCGCAAACTGCGAGCTATGTGCCGCCTGGACTACTGCGGCGGCGG
  CGGCGGCGGGGACCCCGGCGGGGGCCAGCGCGCCTTCACGGCGGCAGCGGGAGCTGCGGGCTGCTGCTGC
  GCGGCGGCGGGAGCGGGCGCCGCCGCGTCCTCGTCCTCATCCTCCGGCTCCCCGCACCTCTACCGCAGCA
  GCAGCGAGCGGCGGCCGACGACGCCGGCGGAGGTGCGCTACATCTCCCCTAAGCACCGACTCATCAAAGT
  GGAGAGCGCGAGCGCTGCGGGGGACCCTCCGGGAGGCGTGTGCTCTGGCGGCCGTACCTGGAGCCCGACG
  ACCTGCGGAGGCAAAAAACTGCTCAACAAGTGCTCCGCCGAGGAGACCGGAGCTGGTCAGAAAGATAAGG
  TGACCATAGCTGATGACTACTCCGATCCCTTTGATGCCAAGAGCGACCTCAAGAGCAAAGCAGGAAAGGG
  TGAGAGCGCTGGCTACATGGAGCCCTACGAAGCCCAGAGGATCATGACAGAATTCCAGAGGCAAGAGAGC
  GTCCGGTCACAGCACAAAGGCATCCAGTTGTACGACACCCCGTACGAGCCGGAAGGCCAGAGCGTGGACT
  CAGACTCAGAGAGCACTGTGAGCCTTCGGCTGCGGGAGAGCAAGCTACCCCAGGATGACGACAGGCCTGC
  TGATGAGTACGACCAGCCGTGGGAATGGAACCGGGTCACCATCCCCGCCCTGGCAGCCCAGTTTAACGGC
  AATGAAAAGCGGCAGTCCTCCCCATCACCTTCTCGGGACCGACGGCGCCAGCTCCGTGCCCCGGGAGGAG
  GCTTCAAGCCCATCAAGCACGGGAGCCCCGAGTTCTGTGGGATTCTGGGGGAGAGAGTGGATCCCACCAT
  CCCACTGGAGAAACAGATATGGTATCACGGGGCCATCAGCAGAAGTGATGCTGAGAACCTGCTGCGACTC
  TGTAAGGAGTGCAGCTACCTTGTCCGGAACAGCCAGACCAGCAAACATGACTACTCCCTCTCCCTGAAGA
  GTAACCAGGGCTTCATGCACATGAAACTGGCCAAGACCAAAGAGAAGTATGTTCTGGGTCAGAACAGCCC
  CCCTTTTGACAGCGTCCCGGAAGTCATCCACTACTACACCACCAGGAAGCTGCCCATCAAAGGGGCCGAG
  CACTTGTCCCTCCTCTACCCAGTGGCTGTGCGGACTCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033306
• Insert Size: 1512 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033306.1, NP_001028478.1
• RefSeq Size: 2762 bp
• RefSeq ORF: 1512 bp
• Locus ID: 230126
• UniProt ID: Q6PD21
• Cytogenetics: 4 B1
• Gene Summary: Adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. May play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. May also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic-FGF and NGF-induced signaling cascades. May also regulate IRS1 and IRS2 signaling in insulin-producing cells (By similarity).[UniProtKB/Swiss-Prot Function]