Mrs2 (NM_001013389) Mouse Untagged Clone

CAT#: MC216752

Mrs2 (untagged) - Mouse MRS2 magnesium homeostasis factor homolog (S. cerevisiae) (Mrs2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mrs2
• Synonyms: Gm902; HPT; Mrs2l; RPT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216752 representing NM_001013389
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATGCCTGCGCTGCCTGCCCGGGCTGCTGCCCCGCGCCGCGCAGCCCCGGAGGGCCCTGTGGACGG
  CGGTCGCGCGCCTGAGCCTCGCTGCCTGCGGCGGCCGCGCGACCCCTTTGCGCTCCCGGAGCCCGAAAGC
  GTCGAGCACAGCCCGCGCTGCAGGTGATGTGCTCCGGTTTAGAACCTCGGATGCCTCTCAAGCCACTTTA
  GCCAGCGTGGCCCAGGTGTTTGCTGTGACAAAGTTTGACAAAGAAGGAAACGTTACCTCTTTTGAGAGGA
  AGAAAACTGAACTATACCATGAGTTAGCACTCCAGGCCAGAGACTTGAGGTTTCAGCACGTGATGAGCAT
  CACTACTAGAAACAACAGGATCATCATGAGGATGGAGTATTTGAAGGCTGTGATAACTCCAGAGTGTCTT
  CTGATACTAGATTATCGGAATTTAAATTTGGAGCACTGGCTGTTTCGGGAACTGCCCTCACAGCTGGCTG
  GAGAAGGTCAGCTGGTCACCTACCCATTACCATTTGAGTTCAGAGCTATTGAAGCACTCCTGCAGTACTG
  GATCAGCACCCTTCGGGGGAGACTAAGCGTCCTGCAGCCACTGATCCTCGAGACCCTGGATGCTCTAGTG
  GACCCCAAGCACTCGTCTGTGGACAGAAGCAAGCTGCATGTCCTACTCCAAAACGGCAAAAGTCTCTCTG
  AGTTAGAAACAGACATTAAAATTTTCAAAGAGTCGATTTTGGAGCTCTTGGATGAGGAGGAGATGCTGGA
  AGAACTCTGTCTGACCAAGTGGAGCGACCCACATGTCTTTGAGAAGAGCAGCACTGGGATCGACCACGCA
  GAGGAGATGGAGCTGCTGCTGGAGAACTACTACCGCTTAGCAGAGGACCTCTCCAACGAGGCGCGCGAGC
  TTCGGGTCCTGATTGACGATTCGCAGAGCATTATCTTCATCAACCTAGACAGCCATCGCAATGTGATGAT
  GAGGTTAAATCTCCAGCTGACCATGGGAACCTTCTCCCTTTCACTCTTTGGTCTGATGGGAGTTGCTTTT
  GGCATGAATTTGGAATCTTCCCTTGAAGAGGACCATCGCGTGTTTTGGCTGGTTACGGGGATTATGTTTA
  TGGGAAGTGGTCTCATCTGGAGGAGGTTGCTCTCATTCCTTGGAAGACAGCTGGAAGCTCCTGTGCCCCC
  TGTGATGACCTCCTTACCTAAAAAGACCCTTCTGGCCAATAGAAGGATGGACGTGAAAAACAGCCTCAGG
  CCGGAAGGACTTGGAGCAAGCAGGACTATCCTGGCAAGCCGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001013389
• Insert Size: 1305 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013389.2, NP_001013407.2
• RefSeq Size: 2161 bp
• RefSeq ORF: 1305 bp
• Locus ID: 380836
• UniProt ID: Q5NCE8
• Cytogenetics: 13 A3.1
• Gene Summary: Magnesium transporter that mediates the influx of magnesium into the mitochondrial matrix. Required for normal expression of the mitochondrial respiratory complex I subunits.[UniProtKB/Swiss-Prot Function]