Afg1l (NM_145743) Mouse Untagged Clone

CAT#: MC216712

Lace1 (untagged) - Mouse lactation elevated 1 (Lace1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Afg1l
• Synonyms: CG8520; Lace1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216712 representing NM_145743
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCTCCTGGTCGCCCTTGGTTACTCTGCGCTCCGCCGCGCGCAGCCGGCTGACTGGGAGAGGTG
  TTGGGTGCGGGGCGCGGGTCGTCGCAATCCCTCCTCCGGCCCCTGGCCCTGGGAAACCCCTGTGGAAAGC
  CTACACAGTCCAGACATCCGAGGGTGTACGCCCAACAGCCGCTTCTGAGGCCCGTTTGAAAGCCTTGGCT
  GTTTGCCACGGGCCTCTGGACCACTACGACTTTCTGATCAAATCTCAGGAGCTGAGGGAGGATGAACATC
  AAAGAAGAGTCGTTCAGTGTTTGCAGAAGTTACAGGAGGACCTTAAAGGATACAGCATAGAGGAAGGAGG
  GCTCTTTTCAAAGCTTTTTTCAAGGAACAAACCTCCAAAGGGCCTCTATGTTTATGGAGACGTTGGTACA
  GGAAAAACAATGGTGATGGACATGTTTTATGCATATGTGGAAACGAAGAGAAAGAAGCGGGTCCATTTTC
  ACGGCTTCATGCTGGACGTGCACAGAAGGATACACCACCTTAAACAGAGTTTGCCAAAAAGGAAGGCGGG
  ATTCATGGCTAAGTCATATGACCCGATAGCTCCCATAGCCGAGGAAATCAGTCAAGAAACTTCTCTCCTA
  TGTTTTGATGAATTTCAGGTCACTGACATCGCCGACGCCATGATTCTGAAACAGCTTTTTGAAAATCTGT
  TCAAAAACGGGGTCGTCGTTGTGGCAACATCCAACAGACCACCGGAAGATCTCTATAAAAATGGACTCCA
  GAGGGCTAACTTTGTGCCATTTATAGCTGTCCTGAAGGAATATTGTGATACGCTCCAGCTGGATTCTGGG
  GTAGATTACCGGAAAAGGGAACTCGCCCCTGCAGGAAAACTCTACTACCTCACAAGTGAGGCTGATGTGG
  AGGCCGTCGTGGATAAGTTGTTTGATGAGCTGGCTCAGAAACAAAATGATTTAACTAGCCCAAGGATTCT
  GAAAGTGCAAGGCAGAGAGCTGCGGCTGAATAAAGCCTGTGGAAGCGTTGCCGACTGCACATTTGAAGAG
  CTGTGTGAGAGACCACTTGGAGCAAGTGACTATTTGGAATTATCAAAGAATTTTGATACGGTAATCATAC
  GAAACATTCCGCAGTTTTCACTGGCAAAGAGGACGCAAGCGAGAAGATTCATCACTCTCATTGATAACTT
  CTATGACTTCAAGGTGCGCATTATCTGCTCCGCTTCAGCTCCGATCTCAAGCTTGTTTCTGCATCAGCAT
  CAGGACAGTGAGTCGGACCAAAGCAGAATCCTGATGGATGATTTGGGGCTGAGCCAGGACTCAGCCGGGC
  TCTCCATGTTTACGGGGGAGGAGGAAATCTTCGCCTTTCAGCGCACCATTTCCCGGCTCACAGAGATGCA
  GACGGAGCAGTACTGGATTGAAGGGGACAGAAGCAGGAAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145743
• Insert Size: 1443 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145743.2, NP_665686.2
• RefSeq Size: 2530 bp
• RefSeq ORF: 1443 bp
• Locus ID: 215951
• UniProt ID: Q3V384
• Cytogenetics: 10 22.88 cM
• Gene Summary: Putative mitochondrial ATPase. Plays a role in mitochondrial morphology and mitochondrial protein metabolism. Promotes degradation of excess nuclear-encoded complex IV subunits (COX4I1, COX5A and COX6A1) and normal activity of complexes III and IV of the respiratory chain. Mediates mitochondrial translocation of TP53 and its transcription-independent apoptosis in response to genotoxic stress.[UniProtKB/Swiss-Prot Function]