Rgs6 (NM_015812) Mouse Untagged Clone

CAT#: MC216490

Rgs6 (untagged) - Mouse regulator of G-protein signaling 6 (Rgs6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rgs6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216490 representing NM_015812
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGGGGTCCGGGGACCAGCGAGCAGTGGGGATCGCTGATCCAGAAGAGAGTTCTCCCAACATGA
  TTGTCTACTGCAAAATTGAGGACATCATTACAAAGATGCAAGATGACAAGACAGGGGGTGTGCCCATCAG
  AACAGTTAAGAGCTTTCTCTCCAAAATCCCCAGTGTCGTCACAGGTACTGACATTGTACAGTGGCTTATG
  AAGAACCTTTCCATTGAGGACCCAGTTGAAGCAATACACCTGGGAAGCCTTATTGCCGCCCAGGGCTACA
  TCTTCCCAATCTCAGACCATGTTCTCACCATGAAGGACGATGGCACCTTTTACCGTTTCCAGGCTCCTTA
  CTTCTGGCCTTCAAACTGCTGGGAACCTGAAAACACGGACTATGCCATCTATCTCTGTAAGAGGACGATG
  CAGAACAAAGCAAGGCTGGAACTGGCCGACTACGAAGCAGAAAACTTAGCAAGACTCCAGAGGGCCTTTG
  CAAGGAAGTGGGAATTCATCTTTATGCAAGCAGAAGCACAAGTGAAGATTGACCGGAAAAAGGATAAGAC
  AGAAAGAAAAATTCTGGATAGCCAAGAACGGGCCTTCTGGGATGTCCACAGGCCAGTGCCAGGCTGTGTG
  AACACAACAGAAATGGATATCAGAAAATGTCGGCGTTTGAAGAATCCACAAAAGGTTAAAAAGTCAGTAT
  ATGGTGTGACAGACGAGACCCAGTCACAGAGTCCAGTGCACATACCAAGCCAGCCAATCAGGAAAACTAC
  AAAAGATGACATCCGAAAACAGATAACGTTTTTGAATGCACAGATTGACAGACATTGTTTGAAAATGTCC
  AAAGTGGCTGAAAGTTTAATCGCTTACACGGAGCAGTATGTGGAGTACGACCCATTCATAACACCAGCAG
  AGCCATCTAATCCTTGGATCAGCGATGACATCACCTTATGGGACATAGAGATGAGCAAAGAGCCCAGCCA
  GCAGCGAGTGAAGCGTTGGGGCTTCTCTTTTGATGAGATACTGAAGGACCAGGTGGGGCGGGACCAGTTC
  CTCAGATTCCTGGAGTCAGAATTCAGCTCAGAAAATCTCAGGTTCTGGCTGTCTGTCCAAGATCTCAAGA
  AGCAACCTCTACAGGACGTGGCCAAGAGGGTGGAGGAAATCTGGCAAGAGTTTCTAGCTCCCGGAGCCCC
  AAGTGCAATCAACTTGGATTCTCACAGCTATGAGATAACCAGTCAGAATGTCAAAGATGGAGGGAGATAC
  ACATTTGAAGATGCCCAGGAGCACATCTACAAGCTGATGAAGAGTGACAGCTATGCCCGCTTCCTACGGT
  CCAACGCTTACCAGGATCTGTTGCTGGCCAAGAAGAAGGGAAAGTCGCTGGCGGGCAAGCGCCTTACGGG
  CCTGATGCAGTCCTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_015812
• Insert Size: 1419 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015812.4, NP_056627.1
• RefSeq Size: 5983 bp
• RefSeq ORF: 1419 bp
• Locus ID: 50779
• UniProt ID: Q9Z2H2
• Cytogenetics: 12 38.14 cM
• Gene Summary: This gene encodes a member of the RGS (regulator of G protein signaling) family of proteins, which are defined by the presence of a RGS domain that confers the GTPase-activating activity of these proteins toward certain G alpha subunits. This protein also belongs to a subfamily of RGS proteins characterized by the presence of DEP (Dishevelled, Egl-10, and Pleckstrin) and GGL (G-protein gamma like)domains, the latter a G beta 5-interacting domain. The RGS proteins negatively regulate G protein signaling, and may modulate neuronal, cardiovascular, lymphocytic activities, and cancer risk. Mice lacking this gene exhibit decreased heart rate. Alternative splicing results in multiple transcript variants, however, the full-length nature of some of these variants is not known. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (1) lacks an alternate in-frame exon in the 3' coding region, compared to variant 3. Variants 1 and 2 encode the same isoform (2), which is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.