Porcn (NM_023638) Mouse Untagged Clone
CAT#: MC216145
Porcn (untagged) - Mouse porcupine homolog (Drosophila) (Porcn), transcript variant Mporc-d, (10ug)
"NM_023638" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Symbol | Porcn |
Synonyms | 2410004O13Rik; AW045557; DXHXS7465e; Mg61; mMg61; Mporc; porc; Ppn |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC216145 representing NM_023638
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCTTCAGCCGCCAGGAATTTTTCCAGCAGCTACTGCAGGGCTGTCTCCTGCCTACTGTCCAAC AGGGCCTTGACCAGATCTGGCTGCTTCTTACCATCTGCTTCGCCTGCCGCCTCCTTTGGAGGCTGGGGTT ACCGTCTTACCTGAAGCATGCAAGCACCGTGGCAGGTGGCTTCTTCAGCCTCTACCACTTCTTCCAGCTG CACATGGTTTGGGTCGTGCTGCTGAGCCTCCTGTGCTACCTCGTGCTGTTCCTCTGCCGACACTCCTCCC ACCGAGGCGTCTTCCTCTCCGTCACCATCCTCATCTACCTGCTCATGGGTGAGATGCACATGGTGGACAC CGTGACATGGCACAAGATGCGAGGGGCCCAGATGATCGTGGCCATGAAGGCGGTGTCTCTGGGCTTCGAC CTGGACCGGGGCGAGGTGGGTGCAGTGCCCTCACCTGTGGAGTTCATGGGCTACCTCTACTTTGTGGGCA CCATCGTCTTTGGGCCCTGGATATCCTTCCACAGCTACCTACAGGCTGTCCAAGGCCGCCCACTGAGCCG CCGATGGCTGAAGAAGGTGGCCCGGAGCCTGGCGCTGGCCCTGCTGTGCCTTGTACTGTCCACTTGTGTG GGCCCCTACCTCTTCCCCTACTTCATCCCCCTCGACGGTGACCGACTCCTTCGCAACAAGAAACGCAAAG CCAGGGGCACCATGGTAAGGTGGCTACGAGCCTACGAGAGTGCTGTCTCCTTCCACTTCAGCAACTATTT TGTGGGCTTTCTGTCTGAGGCCACAGCCACATTGGCCGGGGCTGGCTTCACGGAGGAGAAGGACCACCTG GAATGGGACCTGACAGTCTCTAGACCGTTGAATGTGGAGCTGCCCCGGTCCATGGTGGAAGTTGTCACAA GCTGGAACCTGCCCATGTCTTATTGGTTAAATAATTATGTTTTCAAAAATGCCCTTCGCCTGGGGACCTT CTCTGCTGTGCTGGTCACCTATGCAGCCAGTGCCCTCCTGCATGGCTTCAGTTTCCACCTGGCTGCTGTG CTGCTGTCCCTGGCATTCATCACTTATGTGGAACATGTCCTCAGAAAGCGCCTGGCTCAGATCCTCAGTG CCTGCATCTTGTCAAAGCGTTGTCTGCCAGACTGTTCACATCGGCATCGCTTGGGCCTGGGGGTACGAGC CTTAAACTTGCTCTTTGGGGCCCTGGCTATCTTCCACCTGTCCTACCTGGGCTCCCTGTTTGATGTCGAT GTGGACGACACCACAGAGGAGCAGGGCTACGGCATGGCATACACTGTCCACAAGTGGTCAGAGCTCAGCT GGGCCAGTCACTGGGTCACTTTTGGATGCTGGATCTTCTACCGTCTCATAGGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI |
ACCN | NM_023638 |
Insert Size | 1386 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_023638.3, NP_076127.1 |
RefSeq Size | 1896 bp |
RefSeq ORF | 1386 bp |
Locus ID | 53627 |
UniProt ID | Q9JJJ7 |
Cytogenetics | X 3.7 cM |
Gene Summary | Protein-serine O-palmitoleoyltransferase that acts as a key regulator of the Wnt signaling pathway by mediating the attachment of palmitoleate, a 16-carbon monounsaturated fatty acid (C16:1), to Wnt proteins. Serine palmitoleylation of WNT proteins is required for efficient binding to frizzled receptors.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (d) contains two alternate in-frame exons compared to variant a. The encoded isoform (D) is longer than isoform A. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG226862 | Porcn (tGFP-tagged) - Mouse porcupine homolog (Drosophila) (Porcn) transcript variant Mporc-d, (10ug) |
USD 932.00 |
|
MR226862 | Porcn (Myc-DDK-tagged) - Mouse porcupine homolog (Drosophila) (Porcn), transcript variant Mporc-d |
USD 732.00 |
|
MR226862L3 | Lenti ORF clone of Porcn (Myc-DDK-tagged) - Mouse porcupine homolog (Drosophila) (Porcn), transcript variant Mporc-d |
USD 1,032.00 |
|
MR226862L4 | Lenti ORF clone of Porcn (mGFP-tagged) - Mouse porcupine homolog (Drosophila) (Porcn), transcript variant Mporc-d |
USD 1,032.00 |
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