Ccdc78 (NM_001165929) Mouse Untagged Clone

CAT#: MC215892

Ccdc78 (untagged) - Mouse coiled-coil domain containing 78 (Ccdc78), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ccdc78
• Synonyms: Gm938; Gm950
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215892 representing NM_001165929
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCAGAGGCCAGAACTTCTTTCCTCTATGGAGTATGTGGCATCTCCAGACCCCAAGCCAGGGGTCC
  CACTTCGAGTTGCAGAGAACGTTGCTCCAGGAGCTGAGGACTGGCTGCCAAGTGCTTCTGGGCACCTAGC
  CTGGGCCACTAGCCTGGAGACAGAGCATCAGACACATCTGGAGCTGAGTGAAGAACAGCGGTTGCAGATT
  TCTAAGGAGCTAGTTGATCTGCAGATTGCAACCCACCACCTGCGCGAACAGCATGAAGCTGAAGTCTTTG
  AGCTGAGGAGGGAAATTCTTCGACTGGAAAGTCGGGTGCTAGAGCTAGAACTGCATGGAAATGGTGCCTG
  CCAGGGACATAAAGTCCAACCAATGGCCAATCTGGGGCAACACCAGGTGCCACCATTAGAGCCTCCAGGA
  GGCCAGCAGAAGCTGCAGGAGGAACTGAAATGGCTTCTGGAGCATCACAGAGCCAGGCAGCAGGCACTGG
  AGACACAAGTGGGAGTCCTAAGCCAGCAGTTGCAGGGAGCCCGAGAGGAAGCCAGGACAACTGGTCAGCA
  ACTGGCTTCCCAAGCCATGGTGCTGGCCTCCTGTAAGGGTCAGCTCCGGCAGGCTGAGGCTGAAAACACT
  CAGCTGCAGCTCCAGCTGAAGAAGATGAACGAGGAGTATGCTGTCCGGCTGCAGCACTATGCCAGAGAGA
  CGGTGGAGAACGCCAGTAGCACAAATCAGGCGGCCCTTCAAGCTTTCCTGGAGTCCACTCTGCAGGACAT
  TAGGGCTGCACACCGAACCCGAGAGCAACAGCTGGCCCAAGCTGCCCGGACCTACCGAAAGCGCCTGGCA
  GATCTGAACCAGAGGCAGGAGTTGCTGCTAACCACCTGCAGGGCTACCTTTGCAACAGCCATCAACCTGG
  AGCCATTACCCATGCACTGGGCCACTGAACTCAGCCACCCAAGAGAAAACGAGTATGGCCGACATAGGAC
  ACTGCTACTGTACCCAGAGAAGGGATCTGGTGAAACTTCCAAGGAGAACAAGTCACAGCCACTGGCCCTA
  GACACTGCATCCTGGGCCCAAATCCAGCAGAGGCTGCAGGACTTCTCCCAGGACACTCAGGCAGAGCTAG
  AACGGGAACGGGCACAGCTGATGGTCCGGGCCACCATGGCAGAGCAGCAGCTTTCTGAGCTACAGGAGTA
  TGTGGACCAGCACTTGGGCAGGTACAAGCAGGAAATCTTGAAACTGAGGAAGTTGGTGAATATTGGAGAT
  CCCCAAGGAGTAGAAGCTGTATCTTCGCCGGGTAGTGGTGGCGCACGACTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001165929
• Insert Size: 1314 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001165929.1, NP_001159401.1
• RefSeq Size: 1651 bp
• RefSeq ORF: 1314 bp
• Locus ID: 381077
• UniProt ID: D3Z5T1
• Cytogenetics: 17 A3.3
• Gene Summary: Component of the deuterosome, a structure that promotes de novo centriole amplification in multiciliated cells that can generate more than 100 centrioles. Deuterosome-mediated centriole amplification occurs in terminally differentiated multiciliated cells (G1/0) and not in S phase. Essential for centriole amplification and is required for CEP152 localization to the deuterosome (By similarity).[UniProtKB/Swiss-Prot Function]