Erfe (NM_173395) Mouse Untagged Clone

CAT#: MC212800

Fam132b (untagged) - Mouse family with sequence similarity 132, member B (Fam132b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Erfe
• Synonyms: 4832406C22; Fam132b; myonectin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212800 representing NM_173395
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGACCCGCCGCCCCGTCGGAGCTCGCACGCTGCTCGCCTGCGCCAGCCTACTCGCCGCCATGG
  GCCTCGGTGTCCCTGAGTCCGCGGAGCCCGTGGGGACTCATGCACGCCCGCAGCCGCCCGGGGCCGAGCT
  GCCCGCCCCGCCAGCCAACAGCCCGCCGGAACCCACCATTGCGCATGCACACAGTGTGGATCCCCGGGAT
  GCTTGGATGCTGTTCGTCAAGCAGAGTGACAAGGGGATCAACAGTAAGAGGAGGAGCAAAGCCAGGAGGC
  TGAAGCTTGGCCTGCCAGGACCCCCAGGGCCACCAGGTCCTCAGGGCCCCCCAGGCCCCTTTATCCCATC
  TGAGGTTCTGCTGAAGGAGTTCCAGCTGTTGCTGAAAGGCGCAGTACGGCAGCGAGAGAGCCATCTGGAG
  CACTGCACCAGGGATCTCACTACACCAGCCTCGGGTAGCCCTTCCCGTGTCCCAGCCGCCCAGGAGCTTG
  ATAGCCAGGACCCAGGGGCATTGTTAGCTCTGCTGGCTGCGACCTTGGCCCAGGGCCCGCGGGCACCACG
  TGTGGAGGCCGCATTCCACTGTCGCTTGCGCCGGGATGTGCAGGTGGATCGGCGTGCGTTGCACGAGCTT
  GGGATCTACTACCTGCCCGAAGTTGAGGGAGCCTTCCACCGGGGCCCAGGCTTGAATCTGACCAGCGGCC
  AGTACACCGCACCTGTGGCTGGCTTCTATGCGCTTGCTGCCACTCTGCACGTGGCACTCACCGAGCAGCC
  AAGAAAGGGACCAACACGACCCCGGGATCGTCTGCGCCTGCTGATCTGCATCCAGTCTCTCTGCCAGCAC
  AATGCCTCCCTGGAGACTGTGATGGGGCTGGAGAACAGCAGCGAGCTCTTCACCATCTCAGTAAATGGTG
  TCCTCTATCTACAGGCAGGACACTACACTTCTGTCTTCTTGGACAATGCCAGCGGCTCCTCCCTCACGGT
  ACGCAGTGGCTCTCACTTCAGTGCTATCCTCCTGGGCCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_173395
• Insert Size: 1023 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC056923, AAH56923
• RefSeq Size: 2835 bp
• RefSeq ORF: 1023 bp
• Locus ID: 227358
• UniProt ID: Q6PGN1
• Cytogenetics: 1 D
• Gene Summary: Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin (HAMP) expression in the liver, thereby promoting increased iron absorption and mobilization from stores (PubMed:24880340). Promotes lipid uptake into adipocytes and hepatocytes via transcriptional up-regulation of genes involved in fatty acid uptake (PubMed:22351773).[UniProtKB/Swiss-Prot Function]