Stk24 (NM_145465) Mouse Untagged Clone

CAT#: MC212706

Stk24 (untagged) - Mouse serine/threonine kinase 24 (STE20 homolog, yeast) (Stk24), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Stk24
• Synonyms: 1810013H02Rik; C76483; MST-3; Mst3; STE20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212706 representing NM_145465
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCACTCCCCGGTGCAGTCGGGCCTGCCGGGCATGCAGAACCTGAAAGCAGACCCAGAAGAGCTTT
  TTACCAAGCTAGAGAAGATTGGAAAGGGCTCTTTTGGTGAAGTGTTCAAAGGCATTGACAATCGGACTCA
  GAAAGTGGTGGCCATAAAAATCATTGATCTGGAAGAAGCCGAGGACGAGATAGAGGACATCCAACAAGAG
  ATCACAGTGCTGAGCCAGTGTGACAGTCCCTACGTCACCAAGTACTATGGATCCTATCTCAAGGATACTA
  AGTTGTGGATAATCATGGAGTATCTTGGTGGAGGCTCTGCCCTGGATCTGTTAGAGCCTGGCCCTTTAGA
  TGAAATTCAGATTGCAACCATATTACGAGAAATTCTGAAAGGACTTGATTATCTACACTCGGAGAAGAAA
  ATTCACAGAGATATTAAAGCGGCCAATGTTCTGCTCTCTGAACATGGAGAGGTGAAGCTGGCAGACTTTG
  GAGTGGCCGGCCAGCTGACGGATACCCAGATCAAAAGGAACACCTTCGTGGGTACCCCCTTCTGGATGGC
  GCCGGAGGTCATCAAGCAGTCAGCCTACGACTCAAAGGCAGACATCTGGTCCCTTGGCATCACCGCAATA
  GAACTGGCCAAAGGAGAGCCACCACATTCTGAGCTGCACCCCATGAAGGTGTTATTCCTCATCCCAAAGA
  ACAACCCTCCCACACTGGAAGGGAACTACAGCAAACCCCTCAAGGAGTTCGTGGAGGCCTGCCTGAACAA
  GGAGCCCAGCTTTAGGCCCACTGCTAAGGAATTATTGAAGCACAAATTCATAATCCGCAATGCAAAGAAA
  ACGTCCTACTTGACCGAGCTTATCGACAGGTACAAGAGGTGGAAGGCGGAGCAGAGCCACGAGGACTCCA
  GCTCGGAGGACTCTGACGTGGAGACAGATGGCCAGGCGTCTGGAGGCAGCGACTCTGGGGACTGGATCTT
  CACTATCCGGGAGAAAGATCCCAAGAATCTGGAGAACGGAACTCTTCAGCTCTCGGACTTGGAAAGAAAT
  AAGATGAAAGATATCCCAAAGAGGCCTTTCTCTCAGTGTTTATCCACAATCATTTCTCCTCTGTTTGCGG
  AGCTGAAAGAGAAGAGCCAGGCATGCGGAGGGAACTTGGGGTCAATAGAAGAGCTGCGGGGAGCCATCTA
  CTTGGCGGAAGAGGCCTGCCCTGGGATCTCAGACACTATGGTGGCACAGCTTGTGCAGCGGCTGCAGAGA
  TATTCTCTGAGTGGCGGAGGAGCCTCAGCGCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145465
• Insert Size: 1296 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145465.2, NP_663440.1
• RefSeq Size: 2575 bp
• RefSeq ORF: 1296 bp
• Locus ID: 223255
• UniProt ID: Q99KH8
• Cytogenetics: 14 64.95 cM
• Gene Summary: Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. In association with STK26 negatively regulates Golgi reorientation in polarized cell migration upon RHO activation. Regulates also cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. Acts as a key regulator of axon regeneration in the optic nerve and radial nerve (By similarity).[UniProtKB/Swiss-Prot Function]