Emc9 (NM_033146) Mouse Untagged Clone

CAT#: MC211977

Emc9 (untagged) - Mouse family with sequence similarity 158, member A (Fam158a), (10ug)


  "NM_033146" in other vectors (4)

Reconstitution Protocol

USD 330.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Emc9
Synonyms 1500005A01Rik; Cgi112; Fam158a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC211977 representing NM_033146
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGAGGTGGAGATCTCCGCCCGAGCCTACGGGAAGATGTGTCTGCACGCCTCTCGGTACCCACACG
CTGCTGTCAACGGGTTGTTGCTGGCTCCCGCGACGGGGTCCGGAGAATGCCTCTGCCTCACCGACTGTGT
GCCCCTCTTCCACAGCCATCTGGCCTTGTCCGTCATGCTGGAGGTCGCGCTCAACCAGGTGGATGTGTGG
GGCGCGCAGGCCGGTCTGGTGGTAGCTGGGTACTACCATGCCAATGCGGTTTTGGATGACCAGAGCCCCG
GGCCTCTGGCCTTGAAAATCGCTGGGCGAATTGCAGAATTCTTCCCCAGGGCAGTGCTTATTATGCTGGA
TAATAAGAAATTGGTGACTCGGCCTCGTGTACCTCCAGTCATTGTCCTGGAGAACCAGGGTCTTCAGTGG
GTGCCCAAGGACAAGAACTTAGTGATGTGGAGAGATTGGGAGGAGTCACGACAGATGGTGGGTGCGCTGT
TGGAGGGCCGGGCTCACCAGCATCTTGTGGACTTTGACTGCCATCTTGATGACATTCGGCAGGACTGGAC
CAACCAGCGGCTTAACACTCAAATCACTCAATGGAGTGGTTCCACAGATGGAAATGCCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_033146
Insert Size 621 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_033146.1, NP_149158.1
RefSeq Size 805 bp
RefSeq ORF 621 bp
Locus ID 85308
UniProt ID Q9DB76
Cytogenetics 14 C3
Gene Summary Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes.[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.