Cenpn (NM_028131) Mouse Untagged Clone

CAT#: MC211297

Cenpn (untagged) - Mouse centromere protein N (Cenpn), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cenpn
• Synonyms: 2610510J17Rik; AI426416; AW545024
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211297 representing NM_028131
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAGAATGTTGCCGAGTTTCTCCGGAGGACCATCCTGAAAATCCCTCTGAGTGAGATGAAGAGCA
  TCCTAGAAGCCTGGGACTTTTTGTCTGAAGACCAACTACAAACCATAAACCTGAAGCAGAGAAAGGACTA
  TCTAGCCCAGGAAGTGATTCTGCTATGTGAGGACAAGCGTGCAAGTCTTGATGACGTGGTCCTTTTAGAC
  ATCGTGTATACTCAATTTCATCGGCACCAGAAGCTTTGGAATGTTTTTCAGATGAGTAAGGAACCAGGTG
  AAGATGTCGACCTTTTTGATATGGAACAGTTCCAAAGTTCCTTTAAGAGAATTCTTCAGAGAGCATTAAA
  AAACGTTACAGTCAGCTTCAGAGTCTATGAGAAGGATTCGGTGTGGATCCGAGTTGCCTGGGGAACACAG
  TATTCACAGCCAAACCAGTACAAGCCCACGTTCGTGGTGTATTATCCCCAGACTCCGTATGCCTTCATTT
  CTTCCTGCCATCTGAAGAACACTGTGCCCCTTCTCCATCAGGCACTGAAAGTCGCCAGCAAACACCATCA
  GATTGTGCATTTGGATCTGAGGAGCAGGCACCTGGACTCACTCAAGGCAATTGTTTTTAGAGAGTATAAT
  CAGACCTGTGAAAATTACAGCTCCACAACGTCTCTGCAGGAAGCGAGCCTTTCTATGTGTTTGGATTCAA
  AGATCACTCACGAAAACACAGAAGAAAAAGTGAGGGTCCACCGAGTCACTCAAGAGACCTTTGGGACCTA
  CCCTCAGCCACAGCTAGAGTTCGCACAGTATAAGCTGGAAACCAAGTTCAAGAGTAACATAGGTGGGGGC
  CTCTTGGCCGACAGGAAAGAGCCCTTCCGGTGCCTTGTAAAGTTCTCCAGTCCACATCTTTTGGAAGCCT
  TGAAATCCTTAGCTCCAGCCGGAATTGCAGATGCACCACTTTCTCCCCTGCTCACCTGCATACCGAGCAA
  GAAAATGAATTATTTTAAAATTAGAGATAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028131
• Insert Size: 1014 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028131.3, NP_082407.1
• RefSeq Size: 1768 bp
• RefSeq ORF: 1014 bp
• Locus ID: 72155
• UniProt ID: Q9CZW2
• Cytogenetics: 8 E1
• Gene Summary: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.[UniProtKB/Swiss-Prot Function]