Clybl (NM_029556) Mouse Untagged Clone

CAT#: MC210971

Clybl (untagged) - Mouse citrate lyase beta like (Clybl), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Clybl
• Synonyms: 0610033J05Rik; 2310014M14Rik; AI256068; Clb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210971 representing NM_029556
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTGTGCGTGCTGCGGAACACGGTTCGTGGAGCGGCGGCGCTGCCCAGGCTGAAGGCATCCCACG
  TGGTCAGTGTCTACAAGCCTAGATACAGCTCCTTGTCCAATCACAAGTATGTCCCCCGGAGAGCAGTGCT
  CTATGTCCCTGGGAATGACGAAAAGAAAATAAGGAAGATCCCGTCCTTGAAAGTAGATTGTGCCGTGCTG
  GACTGCGAGGATGGTGTTGCAGAAAACAAAAAGAATGAAGCTCGATTGAGAATAGCAAAAACTCTTGAAG
  ACTTTGACCTGGGCACAACGGAAAAGTGTGTGAGAATCAACTCGGTGTCTAGTGGTCTGGCTGAAGTCGA
  CCTGGAGACATTTCTACAGGCCAGAGTTCTTCCCTCTAGTCTGATGCTACCAAAGGTAGAAGGTCCTGAA
  GAAATCCGATGGTTTTCAGACAAATTTTCACTCCACTTAAAAGGCCGAAAACTTGAACAACCAATGAATT
  TAATCCCCTTTGTGGAGACTGCAATGGGTTTGCTCAATTTTAAGGCAGTGTGTGAAGAAACACTAAAGAC
  TGGACCTCAAGTGGGTCTTTGCCTAGATGCAGTCGTTTTTGGAGGAGAAGATTTTCGAGCCAGCATAGGT
  GCAACAAGCAACAAAGACACCCAAGACATCCTCTATGCCCGGCAGAAGGTTGTTGTCACGGCAAAAGCCT
  TTGGCCTACAAGCCATAGATCTTGTGTACATTGACTTCCGAGATGAAGACGGACTTCTCAGACAGTCAAG
  GGAAGCAGCCGCCATGGGCTTCACTGGTAAACAGGTGATCCACCCTAACCAGATCGCAGTGGTACAGGAA
  CAGTTTACTCCAACCCCAGAAAAAATTCAGTGGGCTGAAGAGCTGATTGCTGCCTTCAAAGAACACCAGC
  AGCTGGGAAAGGGAGCCTTTACTTTCCGAGGAAGTATGATCGACATGCCATTACTGAAGCAGGCCCAGAA
  CATTGTTACGCTTGCCACATCCATCAAGGAAAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029556
• Insert Size: 1017 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029556.3, NP_083832.2
• RefSeq Size: 1257 bp
• RefSeq ORF: 1017 bp
• Locus ID: 69634
• UniProt ID: Q8R4N0
• Cytogenetics: 14 E5
• Gene Summary: Mitochondrial citramalyl-CoA lyase indirectly involved in the vitamin B12 metabolism (PubMed:29056341). Converts citramalyl-CoA into acetyl-CoA and pyruvate in the C5-dicarboxylate catabolism pathway (By similarity). The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, a vitamin B12-poisoning metabolite (PubMed:29056341). Also acts as a malate synthase in vitro, converting glyoxylate and acetyl-CoA to malate (By similarity). Also displays malyl-CoA thioesterase activity. Also acts as a beta-methylmalate synthase in vitro, by mediating conversion of glyoxylate and propionyl-CoA to beta-methylmalate (By similarity). Also has very weak citramalate synthase activity in vitro (By similarity).[UniProtKB/Swiss-Prot Function]