Golph3 (NM_025673) Mouse Untagged Clone

CAT#: MC210408

Golph3 (untagged) - Mouse golgi phosphoprotein 3 (Golph3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Golph3
• Synonyms: 4733401N08Rik; 5730410D03Rik; AW413496
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210408 representing NM_025673
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTCGCTGACCCAGCGGAGCTCGGGCCTGGTGCAGCGGCGCACCGAGGCCTCCCGGAACGCTGCCG
  ACAAGGAGCGGGCGGCGGGAGGCGGCGGCGGCAGCGGCGAGGACGAGGCGCAGAGCCGCCGCGACGAGCA
  GGACGACGACGACAAGGGCGACTCCAAGGAAACGCGGCTGACCCTGATGGAGGAGGTGCTCCTGCTGGGC
  CTCAAGGACCGAGAGGGTTACACATCATTTTGGAATGACTGTATATCATCTGGATTACGTGGCTGTATGT
  TAATTGAATTAGCTTTGAGAGGAAGGTTACAGTTAGAGGCTTGTGGAATGAGAAGAAAAAGTCTTTTAAC
  CAGAAAGGTGATCTGTAAATCGGATGCTCCAACAGGGGATGTTCTTCTTGATGAAGCTCTAAAGCATGTT
  AAGGAGACTCAGCCTCCAGAGACAGTCCAGAACTGGATTGAGTTACTTAGTGGTGAGACGTGGAATCCAT
  TAAAATTGCATTATCAATTAAGAAACGTACGGGAACGATTAGCTAAAAACCTGGTAGAAAAGGGTGTACT
  GACGACAGAGAAACAGAACTTCCTCCTGTTTGACATGACGACACATCCTCTCACCAATAACAACATTAAG
  CAGCGTCTCATCAAGAAGGTACAAGAAGCTGTTCTTGACAAATGGGTAAATGACCCTCACCGAATGGACA
  AGCGCTTGCTGGCCCTCATTTACCTAGCCCATGCCTCGGATGTTTTGGAAAATGCGTTTGCTCCTCTTCT
  GGACGAGCAGTATGATCTAGCCACCAAGAGAGTACGGCAGCTCCTTGACTTAGACCCAGAAGTGGAGTGT
  CTGAAGGCCAACACCAACGAAGTTCTCTGGGCTGTGGTAGCTGCGTTTACCAAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_025673
• Insert Size: 897 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025673.2, NP_079949.1
• RefSeq Size: 2657 bp
• RefSeq ORF: 897 bp
• Locus ID: 66629
• UniProt ID: Q9CRA5
• Cytogenetics: 15 A1
• Gene Summary: Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis (By similarity).[UniProtKB/Swiss-Prot Function]