Dguok (NM_013764) Mouse Untagged Clone

CAT#: MC209782

Dguok (untagged) - Mouse deoxyguanosine kinase (Dguok), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dguok
• Synonyms: dGK; dGK1; dGK2; dGK3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209782 representing NM_013764
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCAGGTCGGTTCCTTCTGCGTCGTCTCCGAGCGTCTTTCCGTTCCCCGCTGCGGAACGCGCTCG
  TGGACGCGCCACACGCCAGGGCCATGCACGACGGGGGCGGCCCCCGAAGGCTCTGCATTGAAGGCAACAT
  CGCTGTGGGCAAATCCACCTTTGTGAAGTTACTCATGAAAACTCACCCGGAGTGGCAGGTGGCGACAGAA
  CCTATAGCAGAGTGGCAGAATATTCAGGCTGCTGGAGCCCAAAAAGATGGCACTTCCAAACGTCTTGGAA
  ACTTGCTAGAAATGATGTACCAGGAGCCAGCACGATGGTCCTACACATTCCAGACCCTTTCTTTCATGAG
  CCGTCTGAAGGTGCAGCTGGAGCCCATCCCAGGGAGACTCCTGCAGGCAGAGAAGTCTGTGCGGGTCTTT
  GAGAGGTCTGTGTACAGTGACAGGTATATCTTTGCAAAGAATCTATTTGAAAATGGCTCCCTCAGTGACA
  TCGAGTGGCACATCTATCAGGACTGGCACTCCTTTCTCCTGCAGGAGTTCGCAAACCGGCTTTTGTTACA
  CGGCTTCATCTATCTCCAGGCTTCACCCCAGGTTTGTATGGAGAGACTCTACCAGAGGGATAGAGAAGAA
  GAAAAGGGCATTGAGCTGGCCTATCTTCAACAGCTACATAGTCAGCACGAAGACTGGTTTATTAACAAGA
  CTACCAAGCTCCACTTCGAAGCTCTGCAGCACGTGCCAGTGCTGGTGCTGGATGTCACTGAAGACTTCTC
  TGAAAATGCCGCCAGGCAGGAAGAGCTCATGGGACAGGTGAACACCTTTATGAGGAACTTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013764
• Insert Size: 834 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013764.2, NP_038792.2
• RefSeq Size: 1110 bp
• RefSeq ORF: 834 bp
• Locus ID: 27369
• UniProt ID: Q9QX60
• Cytogenetics: 6 35.94 cM
• Gene Summary: Phosphorylates deoxyguanosine and deoxyadenosine in the mitochondrial matrix, with the highest efficiency for deoxyguanosine. In non-replicating cells, where cytosolic dNTP synthesis is down-regulated, mtDNA synthesis depends solely on DGUOK and TK2. Phosphorylates certain nucleoside analogs. Widely used as target of antiviral and chemotherapeutic agents.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1), known as dGK-1, represents the longer transcript and encodes the longer isoform (1).