Mapt (NM_001038609) Mouse Untagged Clone

CAT#: MC208988

Mapt (untagged) - Mouse microtubule-associated protein tau (Mapt), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mapt
• Synonyms: AI413597; AW045860; Mtapt; Tau
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208988 representing NM_001038609
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGACCCTCGCCAGGAGTTTGACACAATGGAAGACCATGCTGGAGATTACACTCTGCTCCAAGACC
  AAGAAGGAGACATGGACCATGGCTTAAAAGAGTCTCCCCCACAGCCCCCCGCCGATGATGGAGCGGAGGA
  ACCAGGGTCGGAGACCTCCGATGCTAAGAGCACTCCAACTGCTGAAGACGTGACTGCGCCCCTAGTGGAT
  GAGAGAGCTCCCGACAAGCAGGCCGCTGCCCAGCCCCACACGGAGATCCCAGAAGGAATTACAGCCGAAG
  AAGCAGGCATCGGAGACACCCCGAACCAGGAGGACCAAGCCGCTGGGCATGTGACTCAAGCTCGTGTGGC
  CAGCAAAGACAGGACAGGAAATGACGAGAAGAAAGCCAAGGGCGCTGATGGCAAAACCGGGGCGAAGATC
  GCCACACCTCGGGGAGCAGCCTCTCCGGCCCAGAAGGGCACGTCCAACGCCACCAGGATCCCGGCCAAGA
  CCACGCCCAGCCCTAAGACTCCTCCAGGGTCAGGTGAACCACCAAAATCCGGAGAACGAAGCGGCTACAG
  CAGCCCCGGCTCTCCCGGAACGCCTGGCAGTCGCTCGCGCACCCCATCCCTACCAACACCGCCCACCCGG
  GAGCCCAAGAAGGTGGCAGTGGTCCGCACTCCCCCTAAGTCACCATCAGCTAGTAAGAGCCGCCTGCAGA
  CTGCCCCTGTGCCCATGCCAGACCTAAAGAATGTCAGGTCGAAGATTGGCTCTACTGAGAACCTGAAGCA
  CCAGCCAGGAGGTGGCAAGGTGCAGATAATTAATAAGAAGCTGGATCTTAGCAACGTCCAGTCCAAGTGT
  GGCTCGAAGGATAATATCAAACACGTCCCGGGTGGAGGCAGTGTGCAAATAGTCTACAAGCCGGTGGACC
  TGAGCAAAGTGACCTCCAAGTGTGGCTCGTTAGGGAACATCCATCACAAGCCAGGAGGTGGCCAGGTGGA
  AGTAAAATCAGAGAAGCTGGACTTCAAGGACAGAGTCCAGTCGAAGATTGGCTCCTTGGATAATATCACC
  CACGTCCCTGGAGGAGGGAATAAGAAGATTGAAACCCACAAGCTGACCTTCAGGGAGAATGCCAAAGCCA
  AGACAGACCATGGAGCAGAAATTGTGTATAAGTCACCCGTGGTGTCTGGGGACACATCTCCACGGCACCT
  CAGCAATGTGTCTTCCACGGGCAGCATCGACATGGTGGACTCACCACAGCTTGCCACACTAGCCGATGAA
  GTGTCTGCTTCCTTGGCCAAGCAGGGTTTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001038609
• Insert Size: 1293 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001038609.2, NP_001033698.1
• RefSeq Size: 5396 bp
• RefSeq ORF: 1293 bp
• Locus ID: 17762
• UniProt ID: P10637
• Cytogenetics: 11 E1
• Gene Summary: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (a).