Noct (NM_009834) Mouse Untagged Clone

CAT#: MC208232

Ccrn4l (untagged) - Mouse CCR4 carbon catabolite repression 4-like (S. cerevisiae) (Ccrn4l), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Noct
• Synonyms: AU043840; Ccr4; Ccrn4l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208232 representing NM_009834
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATCAGAGCCCGCGGCGGCTCTGCTCGGCCCTGCTGCTCAGGGACGCCCCCGGCCTGCGCCGCACAC
  TCGTGCCCGGCCCGCGCCGAACGCTCGCCCCGCCGGTGCTCGGATCTCGGCCCAAGTCCCCGCAACTGCA
  GGCGGCGGCGGCCTCGGGCGCCGCGAGGTCGCGTCCCCGAACAGTGAGTTCCATGGGAAACGGCACCAGT
  CGACTCTACAGTGCTCTCGCCAAGACCGTCAACAGCAGTGCAGCTGCTCAGCACCCGGAGTACTTGGTGT
  CAACTGACCCCGAACATCTGGAGCCCATCGATCCTAAGGAACTTCTGGAGGAATGCAGGGCTGTTCTGCA
  CACTCGGCCACCCCGCTACCAGCGGGATTTTGTGGACCTGAGGACAGATTGCTCCAGCAGCCACTCTCCC
  ATTCGCGTCATGCAGTGGAACATCCTCGCCCAAGCTCTCGGAGAAGGCAAAGACAACTTTGTGCAGTGCC
  CCGTGGAAGCGCTCAAATGGGAAGAGAGGAAGTGCCTGATCCTGGAGGAGATCCTGGCTTACCAGCCAGA
  CATACTGTGCCTCCAGGAAGTGGACCACTACTTTGACACCTTCCAGCCACTCCTCAGTAGACTGGGCTAC
  CAAGGCACGTTTTTCCCCAAGCCCTGGTCACCATGTCTAGATGTGGAACACAACAACGGTCCAGATGGCT
  GTGCCTTATTTTTTCTCCAAAACCGATTCAAGCTTATCAGCAGCACCAATATTAGGCTGACAGCCATGAC
  CCTAAAAACCAATCAGGTGGCCATCGCACAGACCCTGGAGTGCAAGGAGTCTGGGCGACAGTTCTGCATT
  GCTGTCACCCACTTAAAAGCCCGCACTGGCTGGGAGCGGTTCCGGTCAGCTCAGGGCTGTGACCTCCTCC
  AGAACCTGCAGAACATCACCCAGGGAGCAAAGATCCCCCTGATCGTCTGCGGGGACTTCAACGCAGAGCC
  AACCGAAGAGGTCTACAAACACTTTGCGTCCTCCAGCCTCAACCTCAACAGCGCCTACAAGCTGCTGAGT
  CCCGATGGACAGTCGGAGCCTCCCTACACAACCTGGAAGATCCGGACCTCAGGCGAGTGTCGCCACACGC
  TGGACTATATCTGGTACTCCAGACATGCTCTGAGTGTCACGTCCGCCCTGGATCTACTCACTGAAGAACA
  GATTGGGCCCAACCGGCTCCCATCCTTCCATTACCCCTCGGACCACCTGTCCCTGGTGTGTGACTTCAGC
  TTTAACGAGGAGCCCCATGAGCTCTTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009834
• Insert Size: 1290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009834.2, NP_033964.1
• RefSeq Size: 3069 bp
• RefSeq ORF: 1290 bp
• Locus ID: 12457
• UniProt ID: O35710
• Cytogenetics: 3 C
• Gene Summary: Represses translation and promotes degradation of target mRNA molecules (By similarity). Plays an important role in post-transcriptional regulation of metabolic genes under circadian control (PubMed:20685873, PubMed:20498072). May have low deadenylase activity and may degrade the poly(A) tails of specific target mRNAs, leading to their degradation and suppression of translation (PubMed:17400819). Exerts a rhythmic post-transcriptional control of genes necessary for metabolic functions including nutrient absorption, glucose/insulin sensitivity, lipid metabolism, adipogenesis, inflammation and osteogenesis (PubMed:20498072, PubMed:22082366, PubMed:21820310, PubMed:22073225, PubMed:22331129). Plays an important role in favoring adipogenesis over osteoblastogenesis and acts as a key regulator of the adipogenesis/osteogenesis balance (PubMed:20498072, PubMed:22082366). Promotes adipogenesis by activating PPARG transcriptional activity in a deadenylase-independent manner by facilitating its nuclear translocation (PubMed:20498072). Regulates circadian expression of NOS2 in the liver and negatively regulates the circadian expression of IGF1 in the bone (PubMed:22073225, PubMed:20685873). Critical for proper development of early embryos (PubMed:23449310).[UniProtKB/Swiss-Prot Function]