Rccd1 (NM_173445) Mouse Untagged Clone

CAT#: MC207944

Rccd1 (untagged) - Mouse RCC1 domain containing 1 (Rccd1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rccd1
• Synonyms: 5830436H09; E430018M08Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207944 representing NM_173445
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGAAACGGCACGGAGCCTGGTTCGGCTTTGGTTTCTGTGGCTTCGGGCAAGCGCTGGGTTCTG
  GGAACAGCCACCATTCAGTGTACAGTCCTGAACCACTGCATGCCAGTGATGATATTTGCCAAGTGAGCGC
  AGGCTGGAGCTACACTGCCCTGGTGACCCGTGGAGGCCGGGTGGAATTGTCAGGTTCTGTGAGCGGTGCA
  GCAGACGGCTGCAGGGACGTGTGGGCTTCGGAGGAACTCCTGGTGCTGCTACGCAACAAGGGCGGCTCCA
  GCACGGAAGTGCAGGCCTGGGTACCAGGTTCTGCGTTACAGGGGGAGCCCCTCTGGGTCCAGAACTTGGT
  TTCTGGGGCAAAGGGCCAAGGGGAAGATGAACCCAGCAGGGAGTCTAGGATGGGGACCCTGCCGTTGCTA
  CCCTGCGCCCGTGCCTATGTGACCCCAGAACCACCCTTCTGCCAGCCTCTGGCCCCAGAGCTGCGGGTCC
  GCCAGCTGGAGCTGGGAGCTGAGCATGTGCTGCTGTTGTGCGCAGCTGGCCAGGTGTTCTCCTGGGGTGC
  GGGCAGGCATGGACAGCTGGGCCACGGGACTCTGGAGGCAGAACTAGAGCCAAGACTGTTGGAGGCACTG
  CAGGGCCTGCGGATGGCTAAGGTGGCTGCAGGTGGCTGGCACTCTGTGTGTTTGAGTGAAACGGGGGATA
  TTTATATCTGGGGCTGGAATGAGTCAGGGCAGCTGGCCCTGCCCACAAGGAGTGGGACAGAGAACAAAGC
  AGAGAGAGAGGAAGCCACAGAATTGAATGAAGATGGTCTCAAAGAGGAATTAGCTGTGGCTGATGCAGGA
  GCTCCTGCCCACTTCATAGCCATCCAGCCCTTCCCTGCTCTTCTGGATCTTCCCCTGGGCTCAGATGCAG
  TTATGGCCAGCTGCGGATCCCGACACACAGCTGTGGTGACACGCACAGGAGAACTCTATACCTGGGGCTG
  GGGTAAATACGGACAGCTTGGCCACAAGGACAGCACCAGCTTGGATCGACCCTGCTGTGTGGAGTACTTT
  GTAGAAAGACAACTTGAAGTAAGGGCTGTGACATGTGGACCCTGGAATACCTATGTCTATGCAATGGAAA
  GAGACAAAAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_173445
• Insert Size: 1134 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173445.4, NP_775621.2
• RefSeq Size: 2340 bp
• RefSeq ORF: 1134 bp
• Locus ID: 269955
• UniProt ID: Q8BTU7
• Cytogenetics: 7 D2
• Gene Summary: Plays a role in transcriptional repression of satellite repeats, possibly by regulating H3K36 methylation levels in centromeric regions together with KDM8. Possibly together with KDM8, is involved in proper mitotic spindle organization and chromosome segregation. Plays a role in regulating alpha-tubulin deacetylation and cytoskeletal microtubule stability, thereby promoting cell migration and TGF-beta-induced epithelial to mesenchymal transition (EMT), potentially through the inhibition of KDM8.[UniProtKB/Swiss-Prot Function]