Selenoi (NM_027652) Mouse Untagged Clone

CAT#: MC207477

Ept1 (untagged) - Mouse ethanolaminephosphotransferase 1 (CDP-ethanolamine-specific) (Ept1), (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Symbol: Selenoi
• Synonyms: 4933402G07Rik; AI448296; AI452230; C79563; D5Wsu178; D5Wsu178e; Ept; Ept1; mKIAA1724; SELI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207477 representing NM_027652
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGCTACGAATACGTGAGCCCAGAGCAGCTGTCGGGCTTTGACAAGTACAAGTACAGCGCTTTGG
  ATACCAACCCACTCTCTCTGTATATCATGCATCCATTTTGGAACACTATAGTGAAGGTGTTTCCTACTTG
  GCTGGCTCCCAATCTTATAACCTTTTCTGGCTTTATGCTGCTTGTGTTCAATTTCCTACTCCTGACATAC
  TTCGACCCTGACTTCTATGCTTCAGCCCCTGGTCATAAGCATGTGCCTGACTGGGTTTGGATTGTCGTGG
  GCATCCTCAACTTTGCTGCCTACACTCTAGATGGAGTGGATGGAAAGCAAGCACGGAGAACCAATTCCAG
  CACCCCGTTAGGGGAGCTGTTTGACCATGGCCTGGACAGTTGGTCGTGTGTTTACTTTGTTGTGACTGTG
  TACTCCATCTTTGGACGAGGACCGACTGGCGTCAGTGTTTTTGTTCTTTATCTCCTGCTATGGGTAGTTT
  TGTTTTCTTTTATCCTGTCTCACTGGGAGAAGTATAACACAGGCGTTCTTTTCCTGCCATGGGGATATGA
  CATTAGCCAAGTGACTATTTCTTTTGTCTACATAGTGACTGCGGTTGTGGGAGTTGAGGCCTGGTATGAA
  CCTTTCCTGTTTAATTTCTTATATAGAGACCTATTCACTGCAATGATTATTGGGTGTGCATTATGTGTGA
  CTCTTCCAATGAGTTTATTAAACTTTTTTAGAAGCTATAAAAGCAACACGCTGAAGCACAAGTCCGTCTA
  TGAAGCCATGGTCCCCTTCTTCTCTCCGTGTTTGCTCTTCACTTTGTGTACAGTGTGGATCCTCTGGTCA
  CCTTCAGATATCTTAGAAATACACCCTAGAATATTCTACTTCATGGTTGGAACAGCTTTTGCCAATATCA
  CATGTCAGCTAATTGTTTGCCAAATGAGCAGCACGCGGTGCCCGACTTTGAACTGGTTACTGCTTCCTCT
  CCTCTTGGTTGTGGCAGCGGTGATCGTAGGTGCAGCCACCTCCCGCCTTGAGAGCGCCCTCCTTTACACA
  CTCACGGCTGCCTTCACTCTGGCTCACATCCATTATGGCGTACAAGTGGTGAAGCAGCTGAGCCGACATT
  TTCAGATTTATCCTTTTTCATTGAGGAAACCAAACTCAGATTGACTAGGAATGGAAGAACAGAATATCGG
  CCTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027652
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027652.3, NP_081928.2
• RefSeq Size: 6806 bp
• RefSeq ORF: 1197 bp
• Locus ID: 28042
• UniProt ID: Q80TA1
• Cytogenetics: 5 16.22 cM
• Gene Summary: The multi-pass transmembrane protein encoded by this gene belongs to the CDP-alcohol phosphatidyltransferase class-I family. It catalyzes the transfer of phosphoethanolamine from CDP-ethanolamine to diacylglycerol to produce phosphatidylethanolamine, which is involved in the formation and maintenance of vesicular membranes, regulation of lipid metabolism, and protein folding. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016]
  Transcript Variant: This variant (1) represents the selenoprotein encoding transcript.