Got1 (NM_010324) Mouse Untagged Clone

CAT#: MC207293

Got1 (untagged) - Mouse glutamate oxaloacetate transaminase 1, soluble (Got1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Got1
• Synonyms: AI789014; cAspAT; cCAT; Got-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207293 representing NM_010324
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCTCCATCAGTCTTTGCCCAGGTTCCGCAAGCTCCTCCGGTTCTGGTCTTTAAGCTCACTGCGG
  ACTTCCGGGATGATCCAGATCCCCGCAAGGTTAACCTCGGCGTGGGAGCGTACCGCACAGATGAATCTCA
  GCCCTGGGTTTTGCCAGTAGTGAGGAAGGTCGAACAGAAGATTGCTAACGACAACAGCCTCAACCACGAG
  TACCTGCCCATCCTGGGCCTGGCAGAGTTCCGGAGCTGTGCTTCTCGCCTAGTTCTTGGGGACAACAGCC
  TGGCTATCAGGGAGAATCGGGTTGGAGGGGTGCAGTCTTTGGGAGGGACAGGCGCTCTTCGGATTGGAGC
  TGACTTCTTAGGGCGATGGTACAATGGTACAGATAACAAGAACACACCAATCTACGTATCATCACCAACC
  TGGGAGAACCATAATGCTGTGTTTTCTGCCGCCGGTTTTAAGGACATTCGGCCCTATTGCTACTGGGATG
  CGGAGAAGAGAGGACTGGACCTCCAGGGTTTCCTGAATGATCTGGAGAATGCCCCCGAGTTCTCCATCTT
  TGTCCTCCATGCCTGTGCGCACAACCCAACAGGGACCGACCCGACTCCAGAGCAGTGGAAGCAGATCGCT
  GCTGTCATGCAGCGCCGTTTTCTGTTCCCCTTCTTTGACTCAGCCTATCAGGGCTTTGCATCTGGAGACC
  TAGAGAAAGATGCGTGGGCTATTCGCTATTTTGTGTCTGAAGGCTTCGAGCTCTTCTGTGCCCAGTCCTT
  CTCCAAGAACTTCGGGCTCTACAATGAGAGAGTGGGGAATCTGACCGTGGTCGGAAAAGAGTCTGACAGC
  GTCCTGCGGGTCCTTTCCCAGATGGAGAAGATTGTACGAATCACCTGGTCCAATCCCCCAGCCCAAGGAG
  CTCGGATTGTGGCCGCCACCCTCTCTGACCCGGAGCTCTTTAAGGAGTGGAAAGGTAACGTGAAGACAAT
  GGCTGACCGGATTCTGACCATGAGATCCGAACTCAGGGCAAGACTAGAAGCTCTCAAGACCCCCGGGACT
  TGGTCTCACATCACTGAGCAGATTGGAATGTTCAGTTTCACCGGCTTGAACCCCAAGCAGGTCGAGTATT
  TGGTCAACGAGAAGCATATCTATCTCCTGCCGAGTGGTCGGATCAACATGTGCGGCTTGACCACCAAGAA
  CCTAGATTACGTCGCTACCTCCATCCATGAAGCCGTCACCAAAATCCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010324
• Insert Size: 1242 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010324.2, NP_034454.2
• RefSeq Size: 2065 bp
• RefSeq ORF: 1242 bp
• Locus ID: 14718
• UniProt ID: P05201
• Cytogenetics: 19 36.67 cM
• Gene Summary: Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain (By similarity).[UniProtKB/Swiss-Prot Function]