Polr2e (BC026842) Mouse Untagged Clone

CAT#: MC207091

Polr2e (untagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide E (cDNA clone MGC:28579 IMAGE:4210827), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Polr2e
• Synonyms: RPB5, XAP4, 25kDa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC026842
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACGAGGAGGAGACCTACCGGCTGTGGAAGATCCGCAAGACGATCATGCAGCTGTGTCATGACC
  GTGGCTACCTGGTGACCCAGGATGAACTGGACCAGACACTTGAGGAATTCAAGGCGCAGTTTGGGGACAA
  GCCCAGCGAAGGACGGCCAAGGCGCACAGACCTCACGGTGCTGGTGGCCCACAACGATGACCCCACAGAC
  CAGATGTTTGTGTTCTTCCCAGAGGAGCCCAAGGTGGGCATCAAGACCATCAAGGTATACTGCCAGCGCA
  TGCAGGAGGAAAACATCACACGGGCGCTGATTGTGGTGCAGCAGGGCATGACGCCCTCCGCCAAGCAGTC
  CCTGGTGGACATGGCCCCGAAGTATGTGCTGGAACAGTTTCTACAGCAGGAGCTGCTCATCAATATCACA
  GAGCACGAGCTAGTCCCTGAGCACGTGGTCATGACAAAGGAGGAGGTGACTGAACTGCTGGCTCGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC026842
• Insert Size: 489 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026842, AAH26842
• RefSeq Size: 1289 bp
• RefSeq ORF: 488 bp
• Locus ID: 66420
• Cytogenetics: 10 C1
• Gene Summary: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity).[UniProtKB/Swiss-Prot Function]