Chkb (BC068195) Mouse Untagged Clone

CAT#: MC206825

Chkb (untagged) - Mouse choline kinase beta (cDNA clone MGC:70277 IMAGE:4164116), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chkb
• Synonyms: CK/EK, CK/EK-beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC206825 representing BC068195.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCCGTTTCCATGGTATGGAGATGCCCTTCACTAAGGAGCCCCGATGGTTGTTTGGGACCATGGAG
  CGGTACCTGAAGCAGATCCAGGACTTGCCTTCCACCAGCCTTCCCCAGATGAACCTGGTAGAGATGTAC
  AGCCTCAAGGACGAGATGAACAGCCTCAGGAAGTTACTAGACGATACCCCGTCACCAGTGGTCTTCTGC
  CACAATGACATCCAGGAAGGAAACATCTTGTTGCTTTCAGAGCCAGACAGTGATGATAACCTCATGTTG
  GTTGATTTTGAGTACAGTAGTTATAACTACAGGGGCTTTGACATTGGGAATCATTTTTGTGAGTGGGTT
  TATGATTATACTTATGAAGAATGGCCTTTCTACAAAGCAAGACCCACGGACTACCCCACTAGAGAACAG
  CAGCTCCATTTTATTCGCCATTATCTGGCAGAGGTTCAGAAGGGTGAGATCCTCTCCGAAGAGGAACAG
  AAGAAACGGGAAGAAGAATTGCTGCTAGAGATCAGTCGGTACTCCCTGGCATCTCACTTTTTCTGGGGT
  CTGTGGTCCACCCTGCAGGCTTCGATGTCCACTATAGAGTTCGGCTACTTGGAGTATGCCCAATCTCGG
  TTCCAGTTCTACTTCCAGCAGAAGGGCCAGCTGACGAGTTCCCCATCATCCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: BC068195
• Insert Size: 675 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC068195
• RefSeq Size: 1278 bp
• RefSeq ORF: 674 bp
• Locus ID: 12651
• Cytogenetics: 15 E3
• MW: 26.8 kDa
• Gene Summary: Has a key role in phospholipid metabolism, and catalyzes the first step of phosphatidylethanolamine and phosphatidylcholine biosynthesis.[UniProtKB/Swiss-Prot Function]