Dhx33 (BC052172) Mouse Untagged Clone

CAT#: MC206713

Dhx33 (untagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 33 (cDNA clone MGC:59423 IMAGE:6515901), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dhx33
• Synonyms: 3110057P17Rik; 9430096J02Rik; Ddx33
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052172
  CCGAGCGGAACTGTCTGCGTCCCTGCCTCCGGCCGCGGTCCTCCCGACATGCCGGAGGAGGCGAGCCTCC CGCCCGCTAAGAGATTCCGGCCGGGCTCCTGCCCTCCCGGGAGACGAGTAGTGATGCTGTTGACGGCTGG CGGCGGCGGCGGAGCTGGAGGAGGCCGGAGGCAGACGCCGCCGCTGGCCCAGCCTTCGGCCAGTCCCTAC CGCGAGGCCCTGGAGCTGCAGCGCCGGAGTCTGCCCATCTTCCGGGCGCGGGGCCAGCTATTGGCTCAGC TCCGAAACCTGGACAACGCGGTCCTCATCGGGGAAACTGGCTCTGGGAAAACAACTCAGATCCCACAGTA CCTCTATGAAGGGGGAATTAGCCGCCAGGGCATCATCGCTGTGACCCAGCCCCGTCGAGTGGCTGCCATC TCTCTTGCCACCAGAGTCTCAGATGAGAAGAGGACAGAACTCGGGAAGCTGGTTGGCTATACAGTGCGCT TTGAGGATGTCACCTCAGAAGACACCAGGATCAAGTTCCTGACAGATGGCATGCTTCTGCGTGAAGCAAT TTCAGACTCCTTGCTTCGGAAGTACAGCTGTGTCATTTTGGACGAGGCCCATGAGCGGACTATTCACACA GATGTGCTCTTTGGAGTGGTGAAGACTGCACAGAAGAGGCGAAAGGAGCTAGGGAAACTGCCTCTCAAAG TGATTGTGATGTCAGCTACCATGGATGTCGACCTGTTTTCTCAGTACTTCAATAGAGCCCCTGTCCTTTA CCTTGAGGGTCGGCAGCATCCAATCCAGATCTTCTATACCAAACAGCCTCAGCAGGATTACCTGCATGCA GCCCTTGTCTCAGTCTTCCAGATCCACCAGGAAGCTCCTGCTTCTCAAGACATCCTGGTGTTCCTCACTG GTCAGGAGGAGATCGAAGCAATGAGCAAGACCTGCAGAGACATCGCAAGGCACCTCCCAGATGGCTGCCC TTCAATGCTCGTCCTTCCTCTGTACGCCTCCCTGCCCTATTCGCAGCAGCTTCGAGTCTTTCAAGGGGCC CCAAAGGGCTATCGCAAAGTGATCATTTCAACCAACATCGCTGAAACCTCCATAACCATTACAGGAATAA AATATGTAGTTGACACGGGCATGGTTAAAGCAAAGAAGTATAACCCTGACAGTGGTCTTGAGGTACTAGC TGTACAGAGAGTATCAAAGACCCAGGCCTGGCAGCGCACAGGCAGGGCTGGGCGAGAGGACAGTGGCATC TGCTACCGACTCTACACTGAGGACGAGTTTGAGAAGTTCGAGAAGATGACGGTGCCAGAGATCCAGAGGT GTAACCTGGCCAGTGTAATACTTCAGCTCCTAGCAATGAAAGTCCCAAATGTGCTCACCTTTGACTTCAT GTCCAAACCTTCTCCAGATCACATTGAGGCGGCCATTGCCCAGCTGGACCTGTTAGGTGCTCTTGAACAT AAGGATGACCAGCTTACCCTGACTCCAATTGGAAGAAAGATGGCAGCATTCCCTTTAGAGCCCAGATTTG CCAAAACTATCCTCCTGTCCTCCAAATTCCACTGTACCGAAGAGATTCTGACCATAGTCTCCCTGCTATC TGTGGACAGTGTCCTTTACAACCCTCCTGCCCGGAGAGATGAGGTGCAGAGTGTCCGGAAGAAATTCATA TCCAGTGAGGGGGATCACATCACCCTGCTCAATATCTATCGGACCTTCAAAAACATCGGTGGGAATAAGG ACTGGTGCAAAGAGAATTTTGTCAACAGCAAGAATATGCTGCTAGTAGCTGAAGTCAGAGCACAGCTGAG AGAAATCTGCTTAAAGATGTCAATGCCAATCATGTCATCTCGAGGGGACATGGAGAGTGTCCGCCGTTGC ATGGCTCACAGCCTCTTTATGAATACTGCTGAACTCCAGACAGATGGCACCTATGCCACCACGGACACAC ATCAGCCAGTGGCCATCCACCCATCATCTGTCCTCTTCCACTGCAAGCCTGCCTGCGTGGTCTACACTTC ACTGCTCTACACCAACAAATGCTACATGCGTGACCTCTGTGTGGTGGATGCCGAGTGGCTCTACGAGGCT GCCCCTGACTACTTCAGGAGGAAGCTGAGAACGGCCAGAAACTGAACCGTTCCAGGATGCTGCCAGCATG GCTGGGCCTGGAGCTTAGATCACAGCTGTCTTCCAAACATAAGTTTGCAGCCAGACTTCCAGACTCCAAG AGCTTAAAAGAATTGCTGAAGCTTCTCAGACTCACCCGTGCAGAATGATCGTACGTAGAGCATGAGAAGC TGAGCTTGTGCCTCGGGTCTCACCAGGCAGTTTTGGAGACTGAATTAACTTCTGAGCCTCAGATTCCTCA TATGCCGGAGTCGGATAAGACTTACACGTTCAGAGTAATGAAGTTTAGAGAAAATGAACAGAATGTGACA GAGACTTGCCTGGTGCAAGATAAGACAATCTGTGACTTGTGAGCCATTGTGCTTTGTGATACTAATTAGG TACAGCACAGCCCCTTGAGCTCTCATGACTGGGGGAGGAGCCATAGGAGACTACAGATCCAATTTGTTGA AATGTTTCTCCCCCCTTCTTACTCCCTCTCCCGTGGATCCCAGAGCCTCATGCATGCTATGCAAAACACT TTGCCACTGAGCCAATCCCCAGCCCTCACTTCTACTTTAATGTCTACCTGTGTGTATTTTAGATAGAAAA TTAAAAAATTGACTCCCACAGACAGTTCCAGAGTAAGGTATATTCTGACCTGTTGACCCTTATGGCTCAG GGTGTAGCACATTGTCTTGTCTGTCTCCAAGCAGAGGCCAGCAAGTAGTTCCTTGGTTGGTCCCCAGTGC CTCTGTGGCTGCATGTCCCCAACAGTGCTTTCAGGAAACCAGCAGGTTTCCTGGAACTAGGAAGCCAACT TTTCAGTGTGGTGGCCTTCTGAAGCCCCGTGGTAGTTTTCTCTTTAACTGAGCACCTACAAATGTGTGCC TGTGAGGGAAGAGAGTTCAAGTCAGAGCTGCTACATGGGGAAGAGGAAGGAGGTACCTGACGGCTCGGAA GCCAGAGGATCAGTTTGTCCTTTCTCCTACACCTAGTCTTAGTTTGGGTTGTTTGTTAGTTTGTTCATTT CTTAAGGGGTAAACCACTTGAGACAGGGTTTCTCTGTGTAGCCCTGCCTGTCCTGAAACTCGCTCTATAG ACCACACTGGCCTCAAACCCAGAGATCAGCCTGCCTTTCCCTCCCAAGTGCTGGGATTGAAGGTGTGCAC CACCACCTGGCCAGTCTTTGTATTTTTGATGCTATTAATTTTCAGTGGTCTGCCTTTGCGGAAACCTGGG GACAATTAGCTTTGCTGCTTCCTAACCAGTCGCCATCTGAAACCTTCGTCGTCTGCACTTGTTAGAGGGA ACTAGAGAAGCCTCGGCATCCTTTTCCCAGTTGGAAAAGAGTCATCTTTGCTGATGACTAGGAGCAAACA TTCCCCAGTTTGGTACTACTTACCTATAATCACTCCCCGCTGAGAAGGGAGGGCTGCGAGTCCTAGGCCA GCCTGGGCTAGGCCTTGTATCTAAGTGAAGGAAGGGGAAGGAACACACTTTCACAGAGAATGTTTGAAGG GGGGGGAGTTTTAGCCCTTTTATTGTGCTTAATCGGTCCAAACACATCTGTACTACTGGGATTTTCTCAT TTATACAGTGAATTGTATTTATAAGCAACAAATGGGCCAAACTGCCTTCAAGGCACTTCGAAGACAGTGG GCAGTTTCTTTGAGTCCAGCAAGCTCTTTCCAGAGAGCCTAGCCTGGCTGGGCACTTCCATTGCCAGGAT CTCATTGGTTCACAGCTGTGTCTGTTGAGTCTGGTCTAGGCTTTATCTAACCCTAGCCGTGGGCAAAGCT TTTAGTCAGTGCTTAGGGCTGTCTGAGTACTCGTGACTACTTTGATAGAAATGGTCACGTTATCAACCTT CTGAAAGGCTCTCCGGAATGTGCTTTCTTTTTCAGTGTATTTTTTGTATTTATGCAAGGTTTATTTAGCC TTGCTTGGTAAGAGGAACTTTTGTATTTTGAAAAGAACATTCATTGTGCTAGTTAAAGAAAACAAGGAGG CAATATATTCCCCAGCAATTAACAGTTTCTGTTCCACTGTAAACTAACAGTAAACTATCGAGGTCTTTTC TGGGTCATGGGAACAGTAGCTTGCAGTGACTGGCTCCAGGGTGAGGCCTAGGACCTTACCCTGGAGGGAC TGCTGCAGAGGACTGGGACCTCCCAGAAAAGGGAGTGATGGATACAGTTTCTAAGGACCCAATGTTTCTG TATAGAAATATGTTTATCATTCTAACATTGTGGGGGGATTTTAATAGAATATTTAGTTTTTTATTAAGCT TTTTTAAAAATTACTATTCAACACAGCTGCTCAGGGATTACATGAGGTGGGAGCCCCATTCTGACTCCAC AGATCACTACGAAGAAATTAATGCTTATCTTCAGCCAAAGGGACAGCTCTCTTCAAGCTTTCTTAAGAAA GTGAAATGTTTTGTGGTTTTGCTGCAGTACAACCTGAGCTACTTTTCAAGTTGGCAAACTCTCCAACCTG AAGACTCCAGCCTGGACAGAATAGCCTCCAACAGACAGATGAACTTCCACAACAGCCCTGCGTGCTGCAG GGGTTTATCTTCTTGGTAACCGTGGCCTGCGACACCCGGGGCACCAGTTCCATTACCAGGATCCCTGTGA GAACATTTGCATCTTTTATAACAGGATTCCAAAACAGCAAAGTATTTACTGTGGCACAAATCCTTGTGCT CCAGCTACCAGGGTCTGTTTGGAACTGTGGCCGCGCATCTAAGATGCCATTCCTTGAGCACATAAACTAG ACTATACCGGTGGGGAAACAATTCTCTATAGACTGCTGTAGTGTAGAAAACTGACAAGCGGATTTGGACA TGTGCTCTGTTCTCTCTGGTCTCAAACATAAAAGAACAGTTCTAGTAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC052172
• Insert Size: 2097 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052172, AAH52172
• RefSeq Size: 5078 bp
• RefSeq ORF: 2097 bp
• Locus ID: 216877
• Cytogenetics: 11 B4
• Gene Summary: Implicated in nucleolar organization, ribosome biogenesis, protein synthesis and cytoplasmic dsRNA sensing (By similarity) (PubMed:21930779). Stimulates RNA polymerase I transcription of the 47S precursor rRNA. Associates with ribosomal DNA (rDNA) loci where it is involved in POLR1A recruitment (PubMed:21930779). In the cytoplasm, promotes elongation-competent 80S ribosome assembly at the late stage of mRNA translation initiation (PubMed:26100019). Senses cytosolic dsRNA mediating NLRP3 inflammasome formation in macrophages and type I interferon production in myeloid dendritic cells (By similarity). Required for NLRP3 activation induced by viral dsRNA and bacterial RNA (By similarity). In dendritic cells, required for induction of type I interferon production induced by cytoplasmic dsRNA via the activation of MAPK and NF-kappa-B signaling pathways (PubMed:24037184).[UniProtKB/Swiss-Prot Function]