Gprk5 (BC019379) Mouse Untagged Clone

CAT#: MC206532

Gprk5 (untagged) - Mouse G protein-coupled receptor kinase 5 (cDNA clone MGC:30358 IMAGE:5116510), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gprk5
• Synonyms: GRK5
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC019379
  GTTTGGCAGCAGCGGCGGCAGCTCGAGCAGCGGCAGCGGCAGCGGCATCATCCCAGACGCTGACAGCACA GCCGGCCGGCTCCCTCGCTGACTGCCGACTGTCAATGGAGCTGGAAAACATCGTGGCCAACACGGTCTTG CTGAAAGCCCGGGAAGGGGGTGGAGGAAAGCGCAAAGGGAAAAGCAAGAAGTGGAAGGAAATCCTGAAGT TTCCTCACATCAGCCAGTGTGAAGACCTCCGAAGGACCATAGACAGAGATTACTACAGTCTCTGTGACAA GCAACCAATTGGGAGACTGCTTTTTCGACAGTTCTGTGAAACCAGGCCTGGGCTGGAGTGCTACATTCAG TTCCTGGACTTAGTGGCAGAATATGAAATTACTCCAGATGAAAACCTTGGGGCGAAGGGGAAGGAAATAA TGACCAAGTACCTCACTCCAAAGTCCCCAGTCTTCATTGCCCAAGTTGGACAGGACCTGGTCTCCCAGAC AGAGAAGAAGCTCCTGCAGAGCCCCTGCAAAGAACTCTTCTCTGCTTGTGCTCAGTCTGTCCATGACTAC TTGACGGGAGACCCCTTCCACGAGTACCTGGATAGCATGTATTTTGACCGTTTTCTGCAGTGGAAATGGT TAGAAAGACAACCAGTGACCAAAAACACTTTCCGGCAGTACCGAGTGCTGGGCAAAGGGGGCTTTGGAGA GGTCTGTGCCTGCCAGGTTCGGGCCACTGGTAAAATGTATGCTTGTAAACGCTTAGAGAAGAAGAGGATC AAAAAGAGGAAAGGCGAATCCATGGCACTCAACGAAAAGCAGATTCTTGAGAAGGTCAACAGCCAGTTTG TGGTCAACCTGGCCTATGCCTATGAAACCAAAGATGCACTATGCCTGGTTCTGACCATTATGAATGGTGG TGACCTGAAGTTTCACATCTACAATATGGGGAATCCTGGCTTTGAGGAAGAGCGAGCCTTATTTTATGCA GCTGAGATCCTCTGTGGCCTAGAAGACTTACACCGTGAGAACACTGTCTATAGAGATCTAAAACCCGAAA ACATCTTGCTGGATGATTATGGCCACATAAGGATCTCAGACCTCGGACTGGCCGTGAAGATCCCCGAGGG AGACCTTATCCGTGGCCGGGTAGGCACTGTTGGCTACATGGCCCCAGAAGTTCTGAACAACCAGCGATAT GGACTGAGCCCTGACTACTGGGGCCTGGGCTGCCTCATCTATGAGATGATTGAAGGCCAGTCACCATTTC GAGGTCGCAAGGAGAAGGTTAAGCGGGAAGAGGTGGATCGCCGGGTGCTGGAGACTGAGGAAGTATATTC CTCCAAGTTCTCTGAAGAGGCCAAGTCCATCTGCAACATGCTGCTCACCAAAGACTCGAAGCAGAGGCTG GGCTGCCAGGAGGAGGGAGCCGCCGAGGTCAAGAGGCACCCCTTCTTCAGGAACATGAACTTTAAGCGCC TGGAGGCTGGGATGTTGGACCCTCCCTTCGTTCCAGATCCCCGGGCTGTATACTGCAAGGATGTGCTGGA CATTGAGCAGTTCTCCACTGTGAAAGGTGTCAACCTGGACCATACGGACGATGATTTTTACTCAAAGTTC TCTACAGGCTCTGTGCCAATTCCATGGCAAAATGAGATGATAGAAACAGAATGTTTCAAGGAGCTGAATG TGTTCGGACCTAACGGTACCCTCTCACCAGACCTGAACAGAAGTCAGCCTCCAGAACCGCCAAAGAAAGG GCTGTTCCACAGACTCTTCAGGCGGCAGCATCAAAGCAATTCCAAGAGTTCACCTACTCCTAAGACCAGT TGTAACCACCGAATAAATTCAAACCACATCAATTCAAACTCCACTGGAAGCAGCTAGTTTCGGCTCTGGC CTTGAAGTCAAAAGTGGAACCAGCTCAGAGCCTTCTACTTGGAAGCAGAATTTATAGCCAGGGGAGCTTC CACTGTGGCTCAGTGGCCAGCAAAGCTCCAGTGGGAACTAAGATAGGAGACCGTTCCCCCAATAACAAAC CTCCAAGTTTCTCAAAGAAATTTCCACTCAGGTCTGTTTTCCAAGGTGGCCCCAAGCTGGGGTGGACTAG AATTTTCCTTGTTGAACATTGCAATAGAAACCCAATGGGATATGACAGCTTGCACGGATTTTAATAGCAT CCTAACTAGAACTGAATTTTGTCTTTATTATTTTTAAAGGAAAGTTTTGTAAATTTCAAAAAAAAAAAAA AA
• Restriction Sites: EcoRI-NotI
• ACCN: BC019379
• Insert Size: 1773 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019379, AAH19379
• RefSeq Size: 2242 bp
• RefSeq ORF: 1773 bp
• Locus ID: 14773
• Cytogenetics: 19 56.52 cM
• Gene Summary: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro).[UniProtKB/Swiss-Prot Function]