Ube2j1 (BC024623) Mouse Untagged Clone

CAT#: MC206377

Ube2j1 (untagged) - Mouse ubiquitin-conjugating enzyme E2, J1 (cDNA clone MGC:25635 IMAGE:4217620), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ube2j1
• Synonyms: Ncube1, Ncube, Ubc6p
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC024623
  GGACTGGGCGGTGTCAGCGGAGGCCCTCGCTAGGCGCGTCCGCCGCGCGAGACCCGGGCAGCTGCGGCGC CGACGAGAGCGGCAGCGGGGACGTCCGCCATGGAGACCCGCTACAACCTGAAGAGCCCGGCCGTTAAGCG ATTGATGAAAGAAGCTGCAGAATTGAAAGATCCAACAGATCATTACCACGCTCAGCCTCTGGAGGATAAC CTTTTTGAATGGCACTTCACAGTTAGAGGGCCCCCAGATTCCGATTTTGATGGAGGAGTTTACCATGGAC GCATAGTACTGCCACCAGAGTACCCCATGAAACCACCAAGCATTATCCTGCTAACAGCTAATGGACGATT TGAAGTAGGCAAGAAGATCTGTTTAAGCATCTCAGGACATCATCCTGAAACCTGGCAGCCTTCATGGAGC ATAAGGACAGCCTTGCTGGCCATCATCGGGTTTATGCCGACTAAAGGAGAGGGGGCCATAGGTTCTCTAG ATTACACACCAGAGGAGAGAAGAGCGCTTGCCAAAAAATCGCAAGATTTCTGTTGTGAAGGCTGTGGCTC TGCCATGAAGGATGTCCTGTTGCCATTAAAATCTGGAAGTGGTTCAAGCCAGGCTGACCAAGAAGCCAAA GAGCTGGCCAGACAGATTAGTTTTAAGGCCGAAGTCAATTCATCTGGAAAGACTATCGCTGAATCAGACT TAAACCAGTGTTTCTCACTAAATGACTCACAAGACGATTTACCTACAACATTCCAGGGTGCTACAGCAAG CACATCGTATGGCGCCCAGAATCCATCAGGAGCACCCTTGCCACAACCTACCCAGCCAGCTCCTAAGAAT ACCTCCATGAGCCCTCGGCAGCGCAGAGCCCAGCAACAGAGTCAGAGAAGGCCATCCACTTCCCCCGATG TGCTCCAAGGCCAGCCACCAAGAGCCCACCACACAGAGCACGGCGGGTCCGCCATGCTGATCATCATCCT GACTTTAGCATTGGCGGCTCTTATATTCCGACGAATATATCTGGCCAATGAGTACATATTTGACTTTGAG TTATAATGCTGGTTTGTGACTTAAGAGCTGTGACTCAGTTGCTTCATTAAACATTCTGCATTGGGTATAA CCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC024623
• Insert Size: 957 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC024623, AAH24623
• RefSeq Size: 1152 bp
• RefSeq ORF: 957 bp
• Locus ID: 56228
• Cytogenetics: 4 A5
• Gene Summary: Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD).[UniProtKB/Swiss-Prot Function]