H2afy2 (BC046794) Mouse Untagged Clone

CAT#: MC206113

H2afy2 (untagged) - Mouse H2A histone family, member Y2 (cDNA clone MGC:61273 IMAGE:5717897), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: H2afy2
• Synonyms: macroH2A2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC046794
  AGAACTGCCGCTTGCCGCCATTGACACGCACAGATACAACCCCAAGAAAGGCAGGGTCCTGCCCAGCACC GGCGGCACGCGGGCCGAACCTGCGGCCGGGATTGGCGCGCACTAGGCCACCGGGCGCCCGGAGCCCGCGG TGCAGCACCAGTATTGTGTAGGTGCCAAGAGACCACTCTGTCAGCAAGCTGAGAGGAAAATCGAGGCAAG ATGTCAGGCCGGAGCGGGAAGAAGAAAATGTCCAAGCTGTCCCGGTCAGCCAGGGCCGGCGTCATCTTTC CAGTAGGCCGGTTGATGCGCTACTTGAAAAAGGGAACCTTCAAGTACAGGATCAGCGTAGGTGCTCCCGT CTACATGGCTGCGGTCATTGAGTACCTAGCAGCGGAAATCCTGGAGTTGGCTGGGAACGCTGCGAGGGAC AACAAGAAGGCACGGATAGCCCCCAGACACATCCTGCTGGCCGTTGCCAACGACGAGGAACTCAACCAGC TACTGAAAGGAGTGACTATCGCCAGTGGGGGTGTCCTGCCGAGAATCCACCCCGAACTGCTGGCCAAGAA GCGAGGGACCAAAGGCAAGTCAGAGACGATCCTCTCCCCACCCCCAGAGAAAAGAGGAAGGAAGGCCGCA TCAGGCAAGAAGGGGGGTAAGAAATCCAAGGCCACCAAGCCACGGACGTCCAAGAAGTCTAAAGCAAAGG ACAGCGATAAAGAAGGAACATCAAATTCCACCTCGGAGGATGGGCCGGGAGATGGCTTCACCATCTTGTC TTCGAAGAGCCTCGTTCTGGGGCAGAAGCTATCCCTGACCCAGAGTGACATCAGCCATATTGGCTCCATG AGGGTGGAGGGCATCGTCCACCCAACCACAGCCGAGATCGACCTCAAGGAAGAGATAGGGAAAGCCTTGG AAAAGGCTGGGGGTAAAGAGTTCTTGGAAACAGTGAAGGAGCTTCGCAAGTCCCAAGGCCCTTTGGAAGT CGCTGAAGCTGCCGTCAGCCAATCCAGTGGACTCGCAGCCAAATTTGTCATCCACTGTCACATCCCCCAG TGGGGCTCCGACAAATGTGAAGAACAGCTGGAAGAGACCATCAAAAACTGCCTGTCTGCAGCAGAGGACA AGAAGCTTAAATCCGTCGCCTTCCCACCGTTCCCCAGTGGCAGAAACTGCTTCCCCAAACAGACGGCCGC CCAGGTGACCCTCAAGGCCATCTCGGCTCACTTCGACGACTCGAGCTCGTCCTCGCTGAAGAATGTGTAC TTCCTGCTCTTCGACAGCGAGAGCATCGGCATCTACGTGCAGGAGATGGCCAAACTGGACACCAAGTAGC TCTCTCCAGTGGCGGCGAAGGAGGAGGATCGGCGTGACGTCACAAGAGCGGGGGTTTTATTTTTTACAAG GATTGCAGAAGGGTGACGGGGCATGGGAGCGGAGGGTGGCTGAGAGGGTGAACGGAGGCAGCGGGAACAG GTCCTGCCTGCCTGGTGAAGGGGTCCGGTGCATTTTATATTCTCTTTACAAAGGGCCTCTCTTCCACAGT TAACCAGGTCTCCACCAGGGCTTTCTTTCCATGTGTTTTTCTTCTTGTTGTTTTAGAACTTTTTCAAAAA CAAACAAGACAAACAAACATAAAAATAGGCCTTCTTTAGATTTATAGCAGTGACTTTTACACACTCTCAC ACACACACACACACACAAAATCTGTTCACAGTTTCTGTGTCTTTGGGTTCCTCCTTTTTCCAAGAGAAAA GGATGTGACTGCCCATCCTAGGTATACTGGCTGTCTCCCTCAGTGGAGAAGATGAGAAGAACATCTTGGG GTGCTTTTCTGTTATAGGGTCCCCTCCCTGACGCCTAAAATATTTGTAACTACACCTAAAAGGGTTTTGC TTCGGGATTTTTGTTTGTTCGGTTTTTTTGTTTTTTTAAAGAAAAAGAAAAATGAGAAGAAAAAAAAAAA AAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC046794
• Insert Size: 1119 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC046794, AAH46794
• RefSeq Size: 1967 bp
• RefSeq ORF: 1119 bp
• Locus ID: 404634
• Cytogenetics: 10 B4
• Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and may participate in stable X chromosome inactivation. [provided by RefSeq, Nov 2015]